BS ISO 1272-2001 Essential oils - Determination of content of phenols《香精油 酚类化合物含量的测定》.pdf

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1、BRITISH STANDARD BS ISO 1272:2000 Essential Oils Determination of content of phenols ICS 71.100.60; 71.040.40 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBS ISO 1272:2000 This British Standard, having been prepared under the direction of the Consumer Products and Services S

2、ector Committee, was published under the authority of the Standards Committee and comes into effect on 15 September 2001 BSI 08-2001 ISBN 0 580 38319 9 National foreword This British Standard reproduces verbatim ISO 1272:2000 and implements it as the UK national standard. The UK participation in its

3、 preparation was entrusted to Technical Committee AW/54, Essential Oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications r

4、eferred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of

5、 a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the

6、interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages

7、 1 to 5 and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsReference number ISO 1272:2000(E) INTERNATIONAL STANDARD ISO 1272 Second edition 2000-09-01 Essential oils Determination

8、of content of phenols Huiles essentielles Dtermination de la teneur en phnolsISO 1272:2000(E) ISO 0002 All rhgits rsevre.de elnUss towrehise scepfidei, on trap of tihs cilbuptanoi mya eb cudorperde ro utzilide ni yna form ro yb yna snaem, eelctinorc ro mecinahcal, inclidugn tohpociypong dna micrfoil

9、m, wittuoh repmissino in writing form eitreh ISO at teh erddass belwo ro ISOs memreb ydob in the ctnuoyr of the rtseuqee.r ISO cirypothg fofice saCe tsopale 65 1121-HC aveneG 20 leT. + 41 22 947 10 11 xaF + 14 22 947 90 74 E-mail cirypothgiso.ch eWb www.iso.ch Printed in Switzlredna ii ISO 0002 All

10、irhgts seredevrISO 1272:2000(E) ISO 0002 All rights rsedevre iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical c

11、ommittees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with t

12、he International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the mem

13、ber bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsibl

14、e for identifying any or all such patent rights. International Standard ISO 1272 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 1272:1973), which has been technically revised.INTERNATIONAL STANDARD ISO 1272:2000(E) ISO 0

15、002 All rights rsedevre 1 Essential oils Determination of content of phenols 1 Scope This International Standard specifies a method for the determination of the percentage, by volume, of phenols in essential oils. NOTE See 8.2.2 which establishes some limits to the application of this method. 2 Norm

16、ative references The following normative documents contain provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements b

17、ased on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of cu

18、rrently valid International Standards. ISO 356:1996, Essential oils Preparation of test sample. ISO 648, Laboratory glassware One-mark pipettes. ISO 1773, Laboratory glassware Narrow-necked boiling flasks. 3P r i n c i p l e The phenolic compounds contained in a known volume of an essential oil are

19、transformed into their alkaline phenol esters, which are soluble in the aqueous phase. The volume of the unabsorbed portion of the essential oil is measured. 4 Reagents Unless indicated otherwise, use only reagents of recognized analytical grade, and distilled or demineralized water or water of equi

20、valent purity. 4.1 Tartaric acid, pulverized. 4.2 Potassium hydroxide, free from silica and alumina, 1 mol/l aqueous solution. 4.3 Xylene, free from impurities soluble in the potassium hydroxide solution (4.2). 5 Apparatus Usual laboratory apparatus and, in particular, the following. 5.1 One-mark pi

21、pettes, of 2 ml and 10 ml capacities, conforming to class A of ISO 648.ISO 1272:2000(E) 2 ISO 0002 All rights rsedevre 5.2 Conical flasks, of 100 ml capacity, conforming to ISO 1773. 5.3 Cassia flask, of 150 ml capacity, with a neck graduated in 0,1 ml, the graduated portion being of 10 ml capacity

22、and at least 150 mm in length. The zero graduation mark shall be a little above the base of the cylindrical portion of the neck. The angle made by the conical wall with the vertical shall be about 30 (see Figure 1). 5.4 Glass rod, of very small diameter. Dimensions in millimetres Graduations in mill

23、ilitres Figure 1 Cassia flask with a graduated neckISO 1272:2000(E) ISO 0002 All rights rsedevre 3 6 Sampling Sampling is not part of the method specified in this International Standard. A recommended sampling method is given in ISO 212. It is important that the laboratory receive a sample which is

24、truly representative and has not been damaged or changed during transport or storage. 7 Preparation of test sample Prepare the test sample according to ISO 356. In order to avoid interphase emulsions at the moment of determination, pretreat the essential oil with tartaric acid as follows: take a vol

25、ume of essential oil greater than 10 ml, then add 0,02 g of tartaric acid (4.1) per millilitre of oil; shake vigorously and without filtering, then dry with magnesium sulfate according to 5.3 of ISO 356:1996. 8 Procedure NOTE If verification of the repeatability is desired, carry out two separate de

26、terminations. 8.1 Test sample Take with the pipette (5.1) 10 ml of the prepared sample (see clause 7) and transfer it to the Cassia flask (5.3) after having introduced approximately 75 ml of the potassium hydroxide solution (4.2). Shake the mixture six times at 5-min intervals. Operate at a constant

27、 temperature of 20 C except in particular cases (see 8.2.2). NOTE The shaking time will be specified in each monograph. 8.2 Determination 8.2.1 Add to the mixture a supplementary quantity of potassium hydroxide (4.2) so that the portion of the oil not transformed into alkaline phenates soluble in th

28、e aqueous phase is raised into the graduated neck of the Cassia flask. Facilitate the separation of the oil drops attached to the walls by rotating the flask between the hands and gently tapping. After allowing the flask to stand for a few hours, read off the volume of unabsorbed oil if all of it is

29、 gathered into the neck. If any emulsion is observed, add 2 ml of xylene (4.3) measured with the pipette (5.1). Agitate the emulsified layer by means of the glass rod (5.4) and allow to stand. If the emulsion disappears, read the volume of unabsorbed oil. If the emulsion persists, repeat the test wi

30、th the addition of 2 ml of xylene to the test portion before the initial shaking. 8.2.2 In some cases heating is required for the determination. In the case of essential oils containing phenol esters, there is sometimes a partial or total saponification of such esters (for example in the case of ess

31、ential oils of clove-containing eugenol acetate, the ester is completely saponified during the measurement).ISO 1272:2000(E) 4 ISO 0002 All rights rsedevre Indications related to the modifications to be introduced in the procedure described in this International Standard, such as shaking time, inter

32、vals between shakes, and temperature, will be stated in the standard specific for the corresponding essential oil in the particular cases indicated above. 9 Expression of results The phenol content, w, of the sample is given, as percentage by volume, by the formula: w=1 0(1 0 V) where V is the volum

33、e, in millilitres, of the unabsorbed portion of the oil read in 8.2.1. If 2 ml of xylene were added, subtract 2 ml from the volume V. Express the result to the nearest whole number. 10 Repeatability The absolute difference between two independent single test results, obtained using the same method o

34、n identical test material in the same laboratory by the same operator using the same equipment within a short interval of time, will in not more than 5 % of cases be greater than 1 %. 11 Test report The test report shall specify: all information necessary for the complete identification of the sampl

35、e; the sampling method used, if known; the test method used, with reference to this International Standard; all operating details not specified in this International Standard, or regarded as optional, together with details or any incidents which may have influenced the test result(s); the test resul

36、t(s) obtained; if the repeatability has been checked, the final quoted result obtained.ISO 1272:2000(E) ISO 0002 All rights rsedevre 5 Bibliography 1 ISO 212, Essential oils Sampling.BS ISO 1272:2000 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent na

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