KS P 1017-2012 The technology for gene expression analysis using DNA microarrays－General requirements and definitions《利用DNA微数列的基因发现分析技术 通用要求事项与术语》.pdf

《KS P 1017-2012 The technology for gene expression analysis using DNA microarrays－General requirements and definitions《利用DNA微数列的基因发现分析技术 通用要求事项与术语》.pdf》由会员分享，可在线阅读，更多相关《KS P 1017-2012 The technology for gene expression analysis using DNA microarrays－General requirements and definitions《利用DNA微数列的基因发现分析技术 通用要求事项与术语》.pdf（14页珍藏版）》请在麦多课文档分享上搜索。

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS P 1017DNA KS P 1017:2012 2012 9 17 http:/www.kats.go.krKS P 1017:2012 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 2012-0423 ： ： ( 02-509-7294) (http:/www.kats.go.kr). 10 5 , . KS P 1017:2012 i ii 1 1 2 1 3 .1 3.1 1 3.2 .2 3.3 3 3.4 .4 4 .4 5 DNA 5 5.1

2、： 5 5.2 6 A( ) .7 8 KS P 1017:2012 ii . DNA DNA DNA , , . DNA , , (dynamic micromirror) (photolithography) , , cDNA . DNA RNA (cDNA) . . , , . , , . KS P 1017:2012 DNA The technology for gene expression analysis using DNA microarrays General requirements and definitions 1 DNA , , DNA DNA , . 2 . . (

3、 ) . KS J 0009, (PCR) KS J ISO 24276, KS P ISO 15189, 3 . 3.1 3.1.1 DNA (DNA microarray) (cDNA ) . 3.1.2 (aminosilane), (aldehyde), (epoxy) . KS P 1017:2012 2 3.1.3 (microarray probe) (3.1.2) (： cDNA, ) KS J 0009 DNA . 3.1.4 (pin) (3.1.2) ( 10 50 m) 3.1.5 (spot) , . (pixel) . 3.2 3.2.1 (genome) (DNA

4、 RNA) 3.2.2 (specimen) , KS P ISO 15189 KS P ISO 15189 “ (primary sample)” “ (specimen)” . 3.2.3 RNA (RNA extraction) RNA KS J ISO 24276 3.2.4 (tissue homogenization) DNA , 3.2.5 (cell lysis) RNA 3.2.6 (absorbance) KS P 1017:2012 3 3.2.7 RNA (RNA purity) - RNA 3.2.8 (reference material) , , , KS J I

5、SO 24276 3.3 3.3.1 (cyanine fluorescent dye) DNA 3.3.2 (target) mRNA , DNA RNA KS J 0009 . 3.3.3 (microarray hybridization) KS J 0009 . 3.3.4 (hybridization solution) 3.3.5 DNA (carrier DNA) , 3.3.6 (repeatability) 2 , KS J ISO 24276 1 (repeatability condition) KS P 1017:2012 4 . KS J ISO 24276 2 (p

6、recision) . KS J ISO 24276 3.3.7 2 (self-to-self test) RNA 2 3.4 3.4.1 (microarray scanner) DNA 3.4.2 (scanning) DNA 3.4.3 (signal) DNA 3.4.4 (microarray scanner image) 3.4.5 (image analysis) 3.4.6 (normalization) , ( ) 3.4.7 (spot quality control) 4 DNA , . KS P 1017:2012 5 (1) RNA (2) , 5 DNA 5.1

7、： 5.1.1 KS J ISO 24276, 6.4 . RNA , a), b) . a) b) (RNase) . . . . ( ) ( ) 1 KS P 1017:2012 6 5.1.2 KS J ISO 24276, 6.6 . . . RNA , . (buffer) . . 5.1.3 KS J ISO 24276, 6.5 , RNA ( , ) RNA . 5.2 5.2.1 DNA . . , DNA . KS P 1017:2012 7 A ( ) . A.1 Cy5 Cyanine 5 5 Cy3 Cyanine 3 3 QC Quality Control UV

8、Ultraviolet DB Database cDNA Complementary DNA CV Coefficient of variance PCR Polymerase Chain Reaction RNase Ribonuclease DNase Deoxyribonuclease cRNA Complementary RNA ssDNA Salmon sperm DNA MML-V Moloney Murine Leukemia Virus KS P 1017:2012 8 1 Sambrook J, Russell DW. Molecular Cloning A laborato

9、ry manual. New York : Cold Spring Harbor Laboratory Press. 2001 2 Schneider J, Buness A, Huber W, Volz J, Kioschis P, Hafner M, Poustka A, Sultmann H. Systematic analysis of T7 RNA polymerase base in vitro linear RNA amplification for use in microarray experiments. BMC Genomics, 2004, vol. 5, p. 29

10、3 Schena M. DNA Microarrays A practical approach. Oxford University Press. 1999 4 Wang X, Ghosh S, Guo SW. Quantitative quality control in microarray image processing and data acquisition. Nucleic Acids Res., 2001, vol. 29, no. 15, p. e75 5 Call DR, Chandler DP, Brockman F. Fabrication of DNA microa

11、rrays using unmodified oligonucleotide probes. Biotechniques, 2001, vol. 30, no. 2, p. 368 372, 374, 376 6 Huang Z, Sanders JC, Dunsmor C, Ahmadzadeh H, Landers JP. A method for UV-bonding in the fabrication of glass electrophoretic microchips. Electrophoresis, 2001, vol. 22, no. 18, p. 3924 3929 7

12、Xiang CC, Brownstein MJ. Fabrication of cDNA microarrays. Methods Mol Biol., 2003, vol. 224, p. 1 7 DNA 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS P 1017:2012KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS The technology for gene expression analysis using DNA microarrays General requirements and definitions ICS 11.040.00