ASTM E934-1994(2015) 1897 Standard Specification for Serological Pipet Disposable Plastic《一次性塑料血清移液管的标准规格》.pdf

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1、Designation: E934 94 (Reapproved 2015)Standard Specification forSerological Pipet, Disposable Plastic1This standard is issued under the fixed designation E934; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revisi

2、on. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.This standard has been approved for use by agencies of the U.S. Department of Defense.1. Scope1.1 This specification covers disposable plasti

3、c serologicalpipets, calibrated “to deliver” when measuring volumes ofliquids.1.1.1 Any institution or individual who reuses a disposablepipet must bear full responsibility for its safety and effective-ness.2. Referenced Documents2.1 ASTM Standards:2D703 Specification for Polystyrene Molding and Ext

4、rusionMaterials (Withdrawn 0)3E542 Practice for Calibration of Laboratory VolumetricApparatusE920 Specification for Commercially Packaged LaboratoryApparatusE921 Specification for Export Packaged Laboratory Appa-ratusE1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United

5、 States Government Procure-ments3. Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 accuracythe expected distribution of mean volumesaround the stated volume.3.1.2 coeffcient of variationthe expected distribution ofindividual volumes around the mean volume.3.1.3 disposable pipetsu

6、ch pipets will only be expected toprovide their specified performance during their original use oroperation.4. Material and Manufacturer4.1 The pipets made to this specification shall be fabricatedfrom crystal grade, uncolored polystyrene, or regrind of same,in accordance with Specification D703.5.

7、Design5.1 Shape0.5, 1.0, and 2.0-cm3pipets shall be straight andof one-piece construction.Any cross section of a pipet taken ina plane perpendicular to the longitudinal axis shall be circular.5.1.1 Pipets of 5.0, 10.0, 25.0, and 50.0 cm3shall be straightand may consist of one, two, or three componen

8、ts, the extrudedpipet barrel, the pulled or injection molded tip, and the plain,pulled, or injection molded top end. Any cross section of apipet taken in a plane perpendicular to the longitudinal axisshall be circular.5.2 Delivery TipsDelivery tips shall be made with agradual taper of 10 to 40 mm. T

9、he tip end shall be reasonablyperpendicular to the longitudinal axis of the pipet, and shall befree of internal flash.5.3 Top EndThe 5, 10, 25.0, and 50.0-cm3sizes shall havea top end with an inside diameter of 2 to 6.5 mm for aminimum distance of 20 mm from the open end, and shall havean overall le

10、ngth of 24 to 28 mm. On all sizes, the top end shallbe suitable for plugging with filtering material. The top may beflat or chamfered and must be without sharp outer edges. Alltop ends shall be reasonably perpendicular to the longitudinalaxis of the pipet. The O.D. of top end should range from 7 to9

11、 mm.5.4 Dimensions and Outflow TimesThe limiting dimen-sions and outflow times shall be as shown in Table 1. Outflowtimes shall be determined by means of a stopwatch onunplugged pipets when using distilled water at 25 6 5C.6. Markings6.1 Graduation MarkingsGraduation lines shall be be-tween 0.2 mm a

12、nd 0.5 mm in width, and in a plane perpen-dicular to the longitudinal axis of the pipet parallel to eachother.Amain graduation line shall extend at least35 of the wayaround the pipet. The values of all main graduation lines shallbe in Arabic numbers directly above the lines referenced.Intermediate g

13、raduation lines shall extend at least15 of the way1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onLaboratory Ware and Supplies.Current edition approved June 1, 2015. Published July 2015. Originally a

14、pprovedin 1983. Last previous edition approved in 2009 as E934 94 (2009). DOI:10.1520/E0934-94R15.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Docu

15、ment Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States1around the pipet, and least graduation lines shall extend at leas

16、t18 of the way around the pipet. The zero graduation line shallbe at least 90 mm below the top of the pipet on all sides.6.2 Identification MarkingsEach pipet shall be markedwith the manufacturers or vendors name or trademark, itscapacity and subdivision, the symbols “TD” (“to deliver”) and“20C”, an

17、d either a wide band or two narrow bands on the topend to signify that the last drop must be blown out to achievefull delivery.7. Capacity7.1 The pipet capacity, expressed as cm3(or mL), and theexpected deviation from the stated capacity, shall be stated onthe product package or published data. The

18、expected deviationfrom stated capacity is expressed as accuracy and coefficient ofvariation. Pipets shall be calibrated for delivery at 20C.7.1.1 The accuracy shall be determined as specified in 7.2and shall be within the limits given in Table 2.7.1.2 The coefficient of variation shall be determined

19、 asspecified in 7.2 and shall be within the limits given in Table 2.7.2 Determination of Accuracy and Coeffcient ofVariationA minimum number of 30 pipets in accordancewith Practice E542, taken at random, shall be testedgravimetrically, using distilled water. Distilled water at 20 61C is drawn into t

20、he pipet and the volume adjusted as in thecase of normal use. This is discharged into a tared weighingbottle and the mass, m, is determined using a balance capableof weighing to 60.0001 g. The volume, vx, is computed fromthe relation:vx5m0.998(1)7.2.1 Accuracy (A)A =(xv1)/v1 100, in percent,x = mean

21、 of sample measurements, andv1= stated capacity or stated volume of pipet.7.2.2 Coeffcient of Variation (cv)cv = 100S/x, in percentS = standard deviation5(x 2 x!2n 2 1x = individual sample measurements,x = mean of sample measurements, andn = number of pipets measured.7.2.3 The use of volumetric stan

22、dards developed from andtraceable to gravimetric standards is permissible if said volu-metric standards were calibrated using weighings and correc-tions as recommended in Practice E542.8. Workmanship, Finish, and Appearance8.1 Pipets shall be free from defects that may impair theirserviceability, su

23、ch as foreign matter or chips that affect thebore.9. Packaging9.1 For packaging, select from Specifications E920, E921,or Practice E1157.10. Keywords10.1 disposable; pipet; plastic; serologicalTABLE 1 Dimensions and Outflow TimesCapacity, cm3Least Value Graduation cm3(mL)Outside DiameterGraduated Po

24、rtion, mmOutflow, sWall Thickness,min, mmMain No. Graduation 0 to min max0.5 0.01 0.1 0.4 4.5 to 4.8 0.5 3.0 0.81.0 0.01 0.1 0.9 4.5 to 4.8 0.5 3.0 0.81.0 0.10 0.1 0.9 4.5 to 4.8 0.5 3.0 0.82.0 0.01 0.1 1.8 5.5 to 6.7 0.5 4.0 0.92.0 0.10 0.2 1.8 5.5 to 6.7 0.5 4.0 0.95.0 0.10 1 4.0 7.6 to 8.4 0.5 7.

25、0 0.910.0 0.10 1 9.0 9.5 to 11.7 0.5 8.0 0.925.0 0.20 or 0.25 1 or 2 23.0 13.0 to 15.0 1.0 10.0 0.750.0 0.25 1 or 2 47.0 17.0 to 19.0 2.0 12.0 0.8TABLE 2 Accuracy and Coefficient of Variation of StatedCapacityCapacity cm3Accuracy + %ACoefficient of VariationEqual to or less than1.5 %0.5 3 1.51.0 3 1

26、.52.0 3 1.55.0 3 1.510.0 3 1.525.0 3 1.550.0 3 1.5AAccuracy and coefficient of variation are expressed as a percentage of the statedcapacity.E934 94 (2015)2ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard

27、. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed

28、every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible te

29、chnical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshoh

30、ocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the Copyright Clearance Center, 222Rosewood Drive, Danvers, MA 01923, Tel: (978) 646-2600; http:/ 94 (2015)3