KS I ISO 6340-2008 Water quality－Detection of salmonella species《水质 沙门氏菌种检测》.pdf

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1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 6340 KS I ISO 6340:2008 2008 11 24 http:/www.kats.go.krKS I ISO 6340:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 1998 12 24 ： 2008 11 24 2008-0794 ： ： ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 6340:2008 Water quality Detection o

2、f salmonella species 1995 1 ISO 6340, Water quality Detection of salmonella species , . . 1 . . . 2 . . ( ) . KS I ISO 5667 1, ( ) KS I ISO 5667 2, 2： KS I ISO 5667 3, 3： KS J ISO 6579, KS M ISO 3696, 3 . 3.1 2 mm 4 mm (colony) (Gram-negative), (oxidase-negative), (facultative anaerobic), (non-spore

3、forming), (rod-shaped) . , . KS I ISO 6340:2008 2 3.2 . 4 4 . 4.1 . . ( ) . 4.2 . / (Rappaport Vassiliadis) . (Salmonella typhi) ( .) (362) 24 . . 4.3 . . / ( ) 4.4 . ( 1 ). 1 1 . 5 . KS M ISO 3696 3 . KS I ISO 6340:2008 3 . pH . pH 1 mol/L . 5.1 5.1.1 5.1.1.1 (NaCl) 12 (Na2HPO4 12H2O) (KH2PO4) 10 g

4、 5 g 9 g 1.5 g 1 000 mL 2 20 g 10 g 18 g 3 g 1 000 mL 5.1.1.2 . pH 7.20.1 . . (1211) (6.1.2) 15 . 3 . 5.1.2 ： / ( ) 5.1.2.1 , , . (NaCl) 3 (K2HPO4 3H2O) (KH2PO4) 4 g 1 g 8 g 0.4 g 0.6 g 1 000 mL 1a 6 (MgCl2 6H2O) 31.7 g 100 mL a (6 ) 28.6 g/L . . KS I ISO 6340:2008 4 2 0.4 g 100 mL 5.1.2.2 . ( 1) (

5、2) 10 mL . pH 5.20.1 . 10 mL . (1151) (6.1.2) 15 . 5.1.3 ： 5.1.3.1 L (Na2HPO4) (NaHSeO3) 5 g 0.01 g 4 g 10 g 4 g 1 000 mL 5.1.3.2 . . , . pH 7.00.2 . . , , . . . , . . . 5.1.4 5.1.4.1 / (Edel Kampelmacher ) 5.1.4.1.1 KS I ISO 6340:2008 5 , (Na2HPO4) (NaH2PO4) 5 g 5 g 1 g 0.6 g 15 g 900 mL 1 ( ) 10 g

6、 10 g 0.09 g 100 mL 2 0.5 g 100 mL 5.1.4.1.2 (1211) (6.1.2) 15 . 1 . 70 (water bath) 20 . (551) . 2 . . (6.7) / ( 1) ( 2) 1 mL pH 7.00.1 . . . 5.1.4.2 5.1.4.2.1 D( ) L( ) (NaCl) (Na2S2O3) 3.5 g 5 g 2.5 g 3 g 7.5 g 7.5 g 5 g 6.8 g 0.8 g 13 g 1 000 mL KS I ISO 6340:2008 6 0.4 g 100 mL 5.1.4.2.2 ( ) 20

7、 mL . pH 7.40.1 . 1 L . . 50 (water bath) (6.7) . 5.1.4.3 (Wilson Blair ) 5.1.4.3.1 , D( ) (Na2HPO4) 7 (FeSO4 7H2O) Bi2(SO3)3 5 g 10 g 5 g 4 g 0.3 g 8 g 15 g 1 000 mL 0.5 g 100 mL 5.1.4.3.2 ( ) 5 mL . . pH 7.60.1 20 mL (6.7) . ( ) . . 5.2 5.2.1 5.2.1.1 KS I ISO 6340:2008 7 , (NaCl)( ) 3 g 5 g 15 g 1

8、 000 mL 5 g 5.2.1.2 . pH 7.00.1 . (1211) (6.1.2) 15 . (6.7) . 5.2.2 /2 (Kligler ) 5.2.2.1 , D( ) (III) (NaCl) (Na2S2O3) 3 g 3 g 20 g 10 g 1 g 0.5 g 5 g 0.3 g 12 g 1 000 mL 0.4 g 100 mL 5.2.2.2 ( ) 6 mL . pH 7.40.1 . (1211) (6.1.2) 15 . 6 mL . (butt) 2.5 cm . 5.2.3 (Christensen ) KS I ISO 6340:2008 8

9、 5.2.3.1 , D( ) (NaCl) (KH2PO4) 1 g 1 g 5 g 2 g 15 g 1 000 mL 1 0.4 g 100 mL 40 g 100 mL 5.2.3.2 ( 1) 3 mL . (1211) (6.1.2) 15 . 50 . ( 2) 50 mL ( 1) 950 mL . 6 mL . pH 6.80.1 . . 5.2.4 L (Falkow ) 5.2.4.1 L D( ) 5 g 3 g 5 g 1 g 1 000 mL 0.5 g 100 mL KS I ISO 6340:2008 9 5.2.4.2 ( ) 3 mL . pH 6.80.1

10、 . 5 mL . (1211) (6.1.2) 10 . 5.3 5.3.1 5.3.1.1 (NaCl) 0.85 g 100 mL 5.3.1.2 . pH 7.00.1 . (1211) (6.1.2) 15 . . 5.3.2 . O Vi H (KS J ISO 6579 ). . 6 . 6.1 6.1.1 ( ) 170 175 1 . 6.1.2 ( ) , (1211) (1151) . KS I ISO 6340:2008 10 6.2 (701) . 6.3 (362) . 6.4 (420.5) . 6.5 pH 25 pH 0.1 . 6.6 3 mm . 6.7

11、90 mm 100 mm . 6.8 47 mm 50 mm 0.45 m . 7 KS I ISO 5667 1, KS I ISO 5667 2, KS I ISO 5667 3 . 2 5 . 24 . 24 . , . 8 8.1 10 mL (2 )(5.1.1.1)1) (6.8) ( )(5.1.1.1) 50 mL . . 10 mL ( ) 50 mL 10 . 1) . KS I ISO 6340:2008 11 (362) 16 20 8.2 . 8.2 (8.1) 0.1 mL / (5.1.2) 10 mL 1 mL 100 mL . (420.5) 18 24 .

12、. . / (5.1.3) (4.2 ). 8.3 8.4 . (8.3) 24 . 8.3 (6.6 ) (8.2 ) . / (5.1.4.1) (5.1.4.2) ( ), (5.1.4.3) (362) 24 ( 48 ). 8.4 (8.3 ) ( 5 ) . / (5.1.4.1) (5.1.4.2) ( ) (5.1.4.3) (5.2.1) . (362) (6.3) 18 24 . . . 8.4.1 ( ) 1 . 90 % . KS I ISO 6340:2008 12 1 /2 /2 /2 (8.4 ) . . 8.4.1.1 / /2 (5.2.2) . (362)

13、(6.3) 24 . (red slants) , . 8.4.1.2 (5.2.3) . (362) (6.3) 24 . ( ). 8.4.1.3 L L (5.2.4) . . (362) (6.3) 24 . . 8.4.2 (KS J ISO 6579) . 8.4.2.1 (5.3.1) . . . KS I ISO 6340:2008 13 30 60 . . . . , . 8.4.2.2 O O (5.3.2) 8.4.2.1 . (polyvalent) (monovalent) . . 8.4.2.3 . 9 , 1 , 8.4.2 . 10 . a) b) c) x m

14、L d) (8.4 ) , (8.4.1) (Species)/ (Serotypes) (8.4.2) 11 . (8.2) ( B 2 ). (5.1.1) 8.18.4 . (Escherichia coli) 108 10 . KS I ISO 6340:2008 14 A ( ) . . , , , . L , , . . KS I ISO 6340:2008 15 B () 1 KS I ISO 8199： 2008, 2 PETERZ. M., WIBERG. C. and NORBERG, P., The effect of incubation temperature and

15、 magnesium chloride concentration on growth of Salmonella in home-made and in commercially available dehydrated Rappaport Vassiliadis broths, J. Appl. Bact. 66(1989), pp. 523 528 135513 7017 (02)60094114 (02)600948878 http:/ KS I ISO 6340:2008 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water quality Detection of salmonella species ICS 07.100.20 Korean Agency for Technology and Standards http:/www.kats.go.kr