NACE TM0194-2014 Field Monitoring of Bacterial Growth in Oil and Gas Systems (Item No 21224).pdf
《NACE TM0194-2014 Field Monitoring of Bacterial Growth in Oil and Gas Systems (Item No 21224).pdf》由会员分享,可在线阅读,更多相关《NACE TM0194-2014 Field Monitoring of Bacterial Growth in Oil and Gas Systems (Item No 21224).pdf(32页珍藏版)》请在麦多课文档分享上搜索。
1、Standard Test Method Field Monitoring of Bacterial Growth in Oil and Gas Systems This NACE International standard represents a consensus of those individual members who have reviewed this document, its scope, and provisions. Its acceptance does not in any respect preclude anyone, whether he or she h
2、as adopted the standard or not, from manufacturing, marketing, purchasing, or using products, processes, or procedures not in conformance with this standard. Nothing contained in this NACE standard is to be construed as granting any right, by implication or otherwise, to manufacture, sell, or use in
3、 connection with any method, apparatus, or product covered by letters patent, or as indemnifying or protecting anyone against liability for infringement of letters patent. This standard represents minimum requirements and should in no way be interpreted as a restriction on the use of better procedur
4、es or materials. Neither is this standard intended to apply in all cases relating to the subject. Unpredictable circumstances may negate the usefulness of this standard in specific instances. NACE assumes no responsibility for the interpretation or use of this standard by other parties and accepts r
5、esponsibility for only those official NACE interpretations issued by NACE in accordance with its governing procedures and policies which preclude the issuance of interpretations by individual volunteers. Users of this NACE standard are responsible for reviewing appropriate health, safety, environmen
6、tal, and regulatory documents and for determining their applicability in relation to this standard prior to its use. This NACE standard may not necessarily address all potential health and safety problems or environmental hazards associated with the use of materials, equipment, and/or operations det
7、ailed or referred to within this standard. Users of this NACE standard are also responsible for establishing appropriate health, safety, and environmental protection practices, in consultation with appropriate regulatory authorities if necessary, to achieve compliance with any existing applicable re
8、gulatory requirements prior to the use of this standard. CAUTIONARY NOTICE: NACE standards are subject to periodic review, and may be revised or withdrawn at any time in accordance with NACE technical committee procedures. NACE requires that action be taken to reaffirm, revise, or withdraw this stan
9、dard no later than five years from the date of initial publication and subsequently from the date of each reaffirmation or revision. The user is cautioned to obtain the latest edition. Purchasers of NACE standards may receive current information on all standards and other NACE publications by contac
10、ting the NACE FirstService Department, 15835 Park Ten Place, Houston, TX 77084-5145 (telephone +1 281-228-6200). Revised 2014-03-08 Revised 2004-11-15 Approved 1994 NACE International 15835 Park Ten Place Houston, Texas 77084-5145 +1 281-228-6200 ISBN 1-57590-192-7 2014 NACE International NACE TM019
11、4-2014 Item No. 21224 TM0194-2014 NACE International i _ Foreword This standard describes field test methods that are useful for estimating bacterial populations, including sessile bacterial populations, commonly found in oilfield systems. The described test methods are those that can be done on sit
12、e and that require a minimum of laboratory equipment or supplies. The described test methods are not the only methods that may be used, but are methods that have been proved to be useful in oilfield situations. This standard is intended to be used by technical field and service personnel, including
13、those who do not necessarily have extensive or specific training in microbiology. However, because microbiology is a specialized field, some pertinent and specific technical information and explanation are provided to the user. Finally, the implications of the results obtained by these test methods
14、are beyond the scope of this standard. The interpretation of the results is site and system specific and may require more expertise than can be provided by this standard. This standard is loosely based on a document produced by the former Corrosion Engineering Association (CEA). CEA operated in the
15、United Kingdom under the auspices of NACE and the Institute of Corrosion (Icorr).(1)This NACE International standard was originally prepared in 1994 by NACE Task Group T-1C-21 under the direction of Unit Committee T-1C, “Corrosion Monitoring in Petroleum Production.” It was revised in 2004 by Task G
16、roup 214, “Bacterial Growth in Oilfield SystemsField Monitoring: Review of NACE Standard TM0194,” which is administered by Specific Technology Group (STG) 31, “Oil and Gas ProductionCorrosion and Scale Inhibition,” and sponsored by STG 60, “Corrosion Mechanisms.” It is issued by NACE under the auspi
17、ces of STG 31. In NACE standards, the terms shall, must, should, and may are used in accordance with the definitions of these terms in the NACE Publications Style Manual. The terms shall and must are used to state a requirement, and are considered mandatory. The term should is used to state somethin
18、g good and is recommended, but is not considered mandatory. The term may is used to state something considered optional. _ (1) Institute of Corrosion (Icorr), P.O. Box 253, Leighton, Buzzard Beds, LU7 7WB, England. TM0194-2014 ii NACE International _ Standard Test Method Field Monitoring of Bacteria
19、l Growth in Oil and Gas Systems Content 1. General 1 2. Sampling Procedures for Planktonic Bacteria . 2 3. Assessment and Sampling of Sessile Bacteria . 4 4. Culture Techniques . 5 5. Evaluation of Chemicals for Control of Bacteria 7 6. Non-Media-Based Field Methods 8 References 9 Bibliography . 11
20、APPENDIXES Appendix A: Glossary (Mandatory) 12 Appendix B: Alternative Methods for Assessing Bacterial Populations (Nonmandatory) . 17 Appendix C: Growth Medium Formulations (Nonmandatory) 19 Appendix D: Membrane Filtration-Aided Bacterial Analyses (Nonmandatory) . 24 Appendix E: Bacterial Culturing
21、 by Serial Dilution (Mandatory) . 25 Appendix F: Example of Use Flow Chart (Nonmandatory) 28 FIGURES Figure E1: Schematic of triplicate serial dilution inoculation (MPN) . 25 Figure F1: Decision Tree for Microbial Testing 28 TABLES Table 1: Results Interpretation Table 6 Table C1: SRB Growth Media 1
22、9 Table C2: Media for Heterotrophic Bacteria . 21 Table C3: Media for Various Types of Phenol Red Broth 21 Table C4: Nitrogen Utilizing Bacteria Media . 22 Table C5: Nitrogen Reducing Bacteria Media 22 Table C6: Methanogenic Media . 22 Table C7: Iron Bacteria Media 23 Table C8: Phosphate-Buffered Sa
23、line (PBS) . 23 Table E1: Single Serial Dilution 27 Table E2: Duplicate Serial Dilution . 27 Table E3: Five Replicate Serial Dilution . 27 _ TM0194-2014 NACE International 1 _ Section 1: General 1.1 Scope 1.1.1 This standard describes field test methods for estimating bacterial populations commonly
24、found in oil and gas systems. Although these techniques have been successful in the oil field, they are not the only methods that are used. Regardless of the method chosen, all techniques should be applied in a consistent manner. It should be recognized that transportation of samples from the field
- 1.请仔细阅读文档,确保文档完整性,对于不预览、不比对内容而直接下载带来的问题本站不予受理。
- 2.下载的文档,不会出现我们的网址水印。
- 3、该文档所得收入(下载+内容+预览)归上传者、原创作者;如果您是本文档原作者,请点此认领!既往收益都归您。
下载文档到电脑,查找使用更方便
10000 积分 0人已下载
下载 | 加入VIP,交流精品资源 |
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- NACETM01942014FIELDMONITORINGOFBACTERIALGROWTHINOILANDGASSYSTEMSITEMNO21224PDF

链接地址:http://www.mydoc123.com/p-835890.html