SANS 5762-2008 Examination for the presence of viable Bacillus anthracis in foods《食品中的炭疽杆菌存在情况检验》.pdf
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1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-20606-2 SANS 5762:2008Edition 2SOUTH AFRICAN NATIONAL STANDARD Examination for the presence of viable Bacillus anthracis in foods Publishe
4、d by Standards South Africa 1 dr lategan road groenkloof private bag x191 pretoria 0001 tel: 012 428 7911 fax: 012 344 1568 international code + 27 12 www.stansa.co.za Standards South Africa SANS 5762:2008 Edition 2 Table of changes Change No. Date Scope Foreword This South African standard was appr
5、oved by National Committee StanSA TC 5140.26, Microbiological evaluation of foods, feeds and beverages, in accordance with procedures of Standards South Africa, in compliance with annex 3 of the WTO/TBT agreement. This document was published in February 2008. This document supersedes SABS SM 762:197
6、5 (first edition). SANS 5762:2008 Edition 2 1 Contents Page Foreword 1 Scope . 3 2 Normative references 3 3 Apparatus 3 4 Media and reagents 3 5 Storage and dispersion of the sample 5 6 Procedure . 5 SANS 5762:2008 Edition 2 2 This page is intentionally left blank SABS 5762:2008 Edition 2 3 Examinat
7、ion for the presence of viable Bacillus anthracis in foods 1 Scope This standard specifies a method for the examination for the presence of viable Bacillus anthracis in foods. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated
8、references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standards can be obtained from Standards South Africa. SANS 171, Glassware and equipment for
9、 microbiological tests. SANS 5553 (SABS SM 553), Media and reagents for microbiological tests. 3 Apparatus The apparatus and equipment shall comply with the requirements of SANS 171. 4 Media and reagents 4.1 General The general requirements for the ingredients and for the preparation of media and re
10、agents shall comply with those given in SANS 5553. 4.2 Nutrient medium 4.2.1 Ingredients Peptone .10 g Beef extract . 5 g Sodium chloride. 5 g NOTE Commercially available nutrient broth of the same recipe may be used. SANS 5762:2008 Edition 2 4 4.2.2 Preparation Dissolve the ingredients in water and
11、 make up to 1 L. Adjust the pH value to 7,2. Dispense 10 mL volumes into bottles and sterilize by autoclaving at a temperature of (03120+) C for 15 min. 4.3 Nutrient agar To the nutrient medium (4.2) add 1,5 % of agar. Heat to dissolve the agar. Dispense 15 mL volumes into bottles and sterilize by a
12、utoclaving at a temperature of (03120+) C for 15 min. NOTE Commercially available nutrient agar of the same recipe may be used. 4.4 Charcoal-gelatin pellets1)4.5 Sterile filter paper strips Cut coarse, low ash filter paper into strips of size 10 mm 100 mm and sterilize these by autoclaving them (in
13、a suitable container) at a temperature of (03120+) C for 20 min. Take a representative sample 2 d after autoclaving and test it for sterility by immersing it aseptically in nutrient medium (see 4.2). Incubate at 37 C 2 C for 72 h. At the end of this time there shall be no evidence of bacterial growt
14、h. 4.6 Sodium chloride solution Dissolve 9 g of sodium chloride in 1 L of water. Dispense 10 mL volumes into bottles and sterilize by autoclaving at a temperature of (03120+) C for 15 min. 4.7 Blood agar 4.7.1 Basal medium ingredients Agar .15 g Peptone .10 g Beef extract .10 g Sodium chloride. 5 g
15、4.7.2 Preparation Dissolve the ingredients in water by boiling, and make up to 1 L. Adjust the pH value to 7,5. Dispense 15 mL volumes into bottles and sterilize by autoclaving at a temperature of (03120+) C for 15 min. Immediately before use, melt the basal medium, cool to 45 C to 50 C, and add to
16、each bottle 1 mL of fresh sterile blood (horse blood gives excellent results). Mix the blood and the basal medium well and aseptically dispense 15 mL volumes into sterile Petri dishes. The blood agar plates, when poured and set, should show no haemolysis. NOTE If commercially available blood agar ba
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