GOST 31960-2012 Water Methods of toxicity determination by growth inhibition of marine unicellular algae Phaeodactylum tricornutum Bohlin and Sceletonema costatum (Greville) Cleve《.pdf

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1、319602012(IS 10253:2006)2014 Phaeodactylum tricornutum Bohlin Sceletonema costatum (Greville) Cleve(ISO 10253:2006, MOD) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 319602012, - 1.0 92 . 1.2 2009 . -, . , , , . 1 - - - , 4 , 2 ( 343 - )3 , ( 54 3 2012 .) : ( 3166) 0

2、0497 ( 3166) 00497 BY KZ KG MD - RU TJ UZ 4 ISO 10253:2006 Water quality Marine algal growth inhibition test with Sceletonema costatum and Phaeodactylum tricornutum ( . Sceletonema costatum Phaeodactylum tricornutum) :- . - .;- , , - , - , -;- . - . 1.52001 ( 3.6). - . (MOD). 53910 20105 12 2012 . 1

3、897- - 1 2014 .6 II 319602012III - , - . () - - . , , 2014null nullnullnullnullnullnullnullnullnullnull nullnullnullnullnullnullnullnullnull null nullnullnullnullnullnullnullnullnull nullnullnull nullnullnullnullnullnullnullnull , 1 12 .23 .34 35 46 15 () .18 . () .20 . () () 22 . () .27 . () .31 .

4、() , .32 . () .33 . () , 34 . () .35 38 319602012IV 3196020121 319602012 (IS 10253:2006) Phaeodactylum tricornutum Bohlin Sceletonema costatum (Greville) CleveWater. Methods of toxicity determination by growth inhibition of marine unicellular algae Phaeodactylum tricornutumBohlin and Sceletonema cos

5、tatum (Greville) Cleve 201401011 , 6 33 /3 - Phaeodactylum tricrnutum Bohlin Sceletonema costatum (Greville) Cleve ( ) :- ( );- ( ). nullnullnullnullnull null , -, - , . , - , . - ( ):- (50) , - () 50 %, (10), () - 10 % 72 (96 ) ( ); 72 ( );- (50) , - () 50 %, (10) - , () 10 % 72 ( ). 1 96 , , - , .

6、2 , , . , (, ; , ) .3 (50) 72 72 50, 96 96 50; (50) 72 72 50.4 .5 , . - . 3196020122 , :- , ;- (20 2) ( - );- 80 %;- 84 106 (630 800 . .);- 6000 10000 ( ). 2 - : / 170252009 - 17.1.5.0585 . . - , 24576 . 2-. 61275 . (II) 4-. 177074 (O 104283, O 478880) . -, , , . 311877 . . 414774 . (III) 6-. 416578

7、 . (II) 5-. 416676 . . 416879 . . 417477 . 7-. 420179 . . 420977 . 6-. 421777 . . 422075 . . 423377 . . 423477 . . 432877 . . 446177 . . 452577 . 6-. 452978 . . 670972 . 914780 . 965675 . . 1065273 . -N,N,N,N- 2- ( ). 1202676 . 191262007 . 241042001* . 2533682 . , 2706586 . 2922791 ( 835-181) . . 1.

8、 318612012 . * 532282008 . 1. - . . 3196020123 , 1 , - , . (), () . , , , , .3 27065, - :3.1 () (cell density): - . () - (/3).3.2 (specific growth rate): (-) .1 1 :1 (1/ )dx dayx dt= , (1) , /3;t , .2 (-1).3.3 (growth medium): , .3.4 (test specimen): (, , ), , , , - , - () 3.5 (sample analyzed): , , - . 1 (test medium).3.6 (control): .3.7 , effective concentration (EC(r)x): - , %- () ( ) . 3.8 , (effective multiplicity of dilution): - (, ) -, %-