DIN EN ISO 5983-2-2009 Animal feeding stuffs - Determination of nitrogen content and calculation of crude protein content - Part 2 Block digestion and steam distillation method (IS.pdf
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1、October 2009DEUTSCHE NORM English price group 12No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 65.120!$Z-v“1551083www
2、.din.deDDIN EN ISO 5983-2Animal feeding stuffs Determination of nitrogen content and calculation of crude proteincontent Part 2: Block digestion and steam distillation method (ISO 5983-2:2009)English version of DIN EN ISO 5983-2:2009-10Futtermittel Bestimmung des Stickstoffgehaltes und Berechnung de
3、s Rohproteingehaltes Teil 2: Blockaufschluss- und Dampfdestillationsverfahren (ISO 5983-2:2009)Englische Fassung DIN EN ISO 5983-2:2009-10SupersedesDIN EN ISO 5983-2:2005-10www.beuth.deDocument comprises pages222 DIN EN ISO 5983-2:2009-10 National foreword This standard has been prepared by Technica
4、l Committee ISO/TC 34 “Food products” (Secretariat: AFNOR, France), Subcommittee SC 10 “Animal feeding stuffs” (Secretariat: NEN, Netherlands) in collaboration with Technical Committee CEN/TC 327 “Animal feeding stuffs Methods of sampling and analysis” (Secretariat: NEN, Netherlands). Based on the r
5、esults of parallel voting, ISO 5983-2:2009 was adopted as a European Standard. The responsible German body involved in its preparation was the Normenausschuss Lebensmittel und landwirtschaftliche Produkte (Food and Agricultural Products Standards Committee), Technical Committee NA 057-03-03 AA Futte
6、rmittel. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 385 DIN EN ISO 385 ISO 5275-1 DIN ISO 5725-1 ISO 5275-2 DIN ISO 5725-2 ISO 5983-1 DIN EN ISO 5983-1 ISO 6497 DIN EN ISO 6497 ISO 8655-2 DIN EN ISO 8655-2 ISO 8655-3 DIN EN ISO 865
7、5-3 ISO 8655-5 DIN EN ISO 8655-5 Amendments This standard differs from DIN EN ISO 5983-2:2005-10 as follows: a) Clause 11 “Precision” has been revised. b) A second interlaboratory test has been included in Annex A. c) The results of the second interlaboratory test are given in a new Table A.2. d) Fi
8、gure A.1 “Relationship between precision values and the mean crude protein content” has been added. e) Annex B has been modified to include the “Results of a proficiency test; comparison of the colorimetric and potentiometric endpoint determination of the titration”. f) The standard has been brought
9、 in line with the current rules of presentation. Previous editions DIN EN ISO 5983-2: 2005-10 3 National Annex NA (informative) Bibliography DIN EN ISO 385, Laboratory glassware Burettes DIN EN ISO 5983-1, Animal feeding stuffs Determination of nitrogen content and calculation of crude protein conte
10、nt Part 1: Kjeldahl method DIN EN ISO 6497, Animal feeding stuffs Sampling DIN EN ISO 8655-2, Piston-operated volumetric apparatus Part 2: Piston pipettes DIN EN ISO 8655-3, Piston-operated volumetric apparatus Part 3: Piston burettes DIN EN ISO 8655-5, Piston-operated volumetric apparatus Part 5: D
11、ispenser DIN ISO 5725-1, Accuracy (trueness and precision) of measurement methods and results Part 1: General principles and definitions DIN ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibilit
12、y of a standard measurement method DIN EN ISO 5983-2:2009-10 4 This page is intentionally blank DIN EN ISO 5983-2:2009-10 EUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 5983-2June 2009ICS 65.120 Supersedes EN ISO 5983-2:2005 English VersionAnimal feeding stuffs - Determination of nitrogen con
13、tent andcalculation of crude protein content - Part 2: Block digestion andsteam distillation method (ISO 5983-2:2009)Aliments des animaux - Dosage de lazote et calcul de lateneur en protines brutes - Partie 2: Mthode de digestionen bloc et distillation la vapeur (ISO 5983-2:2009)Futtermittel - Besti
14、mmung des Stickstoffgehaltes undBerechnung des Rohproteingehaltes - Teil 2:Blockaufschluss- und Dampfdestillationsverfahren 5983-2:2009)This European Standard was approved by CEN on 30 May 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions f
15、or giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official
16、 versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgi
17、um, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STA
18、NDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: Avenue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 5983-2:2009: E(ISO2 Contents Page Foreword .3 1 Sco
19、pe4 2 Normative references4 3 Terms and definitions .5 4 Principle .5 5 Reagents 5 6 Apparatus.7 7 Sampling 7 8 Preparation of test sample .8 9 Procedure.8 9.1 General .8 9.2 Test portion8 9.3 Determination 8 9.4 Blank test .9 9.5 Recovery tests .10 10 Calculation and expression of results 10 10.1 C
20、alculation .10 10.2 Calculation of crude protein content.11 10.3 Expression of crude protein content results11 11 Precision 11 11.1 Interlaboratory tests11 11.2 Repeatability 12 11.3 Reproducibility 12 12 Test report12 Annex A (informative) Results of interlaboratory tests 13 Annex B (informative) R
21、esults of a profiency test; comparison of the colorimetric and potentiometric endpoint determination of the titration.17 Bibliography18 DIN EN ISO 5983-2:2009-10 EN ISO 5983-2:2009 (E) 3 Foreword This document (EN ISO 5983-2:2009) has been prepared by Technical Committee ISO/TC 34 “Food products“ in
22、 collaboration with Technical Committee CEN/TC 327 “Animal feeding stuffs - Methods of sampling and analysis”, the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest
23、 by December 2009, and conflicting national standards shall be withdrawn at the latest by December 2009. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all s
24、uch patent rights. This document supersedes EN ISO 5983-2:2005. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finl
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