BS 6068-5 25-1999 Water quality - Biological methods - Determination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms - Section 5 25 .pdf
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1、BRITISH STANDARD BS6068-5.25: 1999 ISO 15522: 1999 Water quality Part 5: Biological methods Section 5.25: Determination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms ICS 13.060.70BS6068-5.25:1999 This British Standard, having been prepared under the
2、directionof the Health andEnvironment Sector Committee, was published underthe authority of the Standards Committee and comesinto effect on 15 August 1999 BSI 03-2000 ISBN 0 580 32866 X Amendments issued since publication Amd. No. Date CommentsBS6068-5.25:1999 BSI 03-2000 i Contents Page National fo
3、reword ii Foreword iii Text of ISO 15522 1BS6068-5.25:1999 ii BSI 03-2000 National foreword This British Standard reproduces verbatim ISO 15522:1999 and implements it as the UK national standard. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Sub
4、committee EH/3/5, Biological methods, which has the responsibility to: aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and
5、 European developments and promulgate them in the UK. A list of organizations represented on this subcommittee can be obtained on request to its secretary. This is one of a series of standards on water quality, others of which have been, or will be, published as Sections of BS 6068. The various Sect
6、ions of BS 6068 are comprised within Parts 1 to 7, which, together with Part 0, are listed below. Part 0: Introduction; Part 1: Glossary; Part 2: Physical, chemical and biological methods; Part 3: Radiological methods; Part 4: Microbiological methods; Part 5: Biological methods; Part 6: Sampling; Pa
7、rt 7: Precision and accuracy. NOTEThe tests described in this British Standard should only be carried out by suitably qualified persons with an appropriate level of biological expertise. Standard biological procedures should be followed throughout. Cross-references The British Standards which implem
8、ent international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purpor
9、t to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cov
10、er, pagesi andii, theISO title page, pagesii toiv, pages 1 to 10 and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.BS6068-5.25:1999 ii BSI 03-2000 Contents Page Forew
11、ord iii Introduction 1 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Principle 2 5 Test environment 2 6 Reagents 2 7 Inoculum 3 8 Apparatus 3 9 Procedure 3 10 Expression of results 5 11 Interpretation of results 5 12 Reproducibility 5 13 Test report 6 Annex A (informative) Examples
12、of contents of test and control flasks 7 Annex B (informative) Typical growth curve using 3,5-dichlorophenol as test material 8 Annex C (informative) Determination of lowest ineffective dilution of wastewater 9 Bibliography 10 Figure B.1 Typical growth curve using 3,5-dichlorophenol as test material
13、 8 Table 1 Results of a ring test 6 Table C.1 9BS6068-5.25:1999 BSI 03-2000 iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
14、ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates
15、 closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circul
16、ated to the member bodies for voting. Publication as an International Standard requires approval by at least 75% of the member bodies casting a vote. International Standard ISO 15522 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. Annex A, Annex
17、B and Annex C of this International Standard are for information only.iv blankBS6068-5.25:1999 BSI 03-2000 1 Introduction Information generated by this method may be helpful in estimating the effect of a test material on mixed bacterial communities in aerobic biological wastewater treatment systems
18、and in choosing suitable initial concentrations for aerobic biodegradability tests The results of this test should be considered only as a guide to the likely toxicity of the test material, since activated sludge from different sources, or even from the same source taken at different times, may diff
19、er in bacterial composition and concentration. Also, laboratory tests cannot truly simulate environmental conditions. For example, no account is taken of longer term adaptation of the microorganisms to the test material or of materials which may adsorb onto biofilm or activated sludge in subsequent
20、wastewater treatment and build up to a toxic concentration over a longer period of time. WARNING Take appropriate precautions when handling sewage, as it may contain potentially pathogenic organisms. Handle with care all toxic test materials or those whose properties are unknown. 1 Scope This Intern
21、ational Standard specifies a method for assessing the potential toxicity of a test material to the growth of aerobic bacteria present in activated sludge. The inhibitory effect is restricted to those microorganisms capable of growth on the chosen organic test medium. This method gives information on
22、 inhibitory effects on the microorganisms over incubation periods up to6 h. This method is applicable to water, wastewater and chemical substances which are soluble under the conditions of the test. Special care is needed with volatile or coloured materials and materials which form turbid suspension
23、s or dispersions. NOTE 1Results with volatile test material should be interpreted with caution and are likely to underestimate any inhibitory effect because of the difficulties in maintaining the initial concentration in the test flasks. NOTE 2Coloured materials and materials of low water-solubility
24、 which form turbid suspensions or dispersions can be tested in many cases using colour/turbidity controls as blanks (see 9.4). NOTE 3Inhibitory effects on activated sludge microorganisms of test materials for which this test is not applicable can be determined by using an inhibition test based on re
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