EN 26461-2-1993 en Water Quality - Detection and Enumeration of the Spores of Sulfite- Reducing Anaerobes (Clostridia) - Part 2 Method by Membrane Filtration《水质 还原亚硫酸盐厌氧菌芽胞探测和计数 第2.pdf
《EN 26461-2-1993 en Water Quality - Detection and Enumeration of the Spores of Sulfite- Reducing Anaerobes (Clostridia) - Part 2 Method by Membrane Filtration《水质 还原亚硫酸盐厌氧菌芽胞探测和计数 第2.pdf》由会员分享,可在线阅读,更多相关《EN 26461-2-1993 en Water Quality - Detection and Enumeration of the Spores of Sulfite- Reducing Anaerobes (Clostridia) - Part 2 Method by Membrane Filtration《水质 还原亚硫酸盐厌氧菌芽胞探测和计数 第2.pdf(5页珍藏版)》请在麦多课文档分享上搜索。
1、CEN ENL264bL-2 93 m 3404589 0042975 b9T m EUROPEAN STANDARD EN 26461-2 NORME EUROPENNE EUROPISCHE NORM January 1993 UM: 628.U.3 : 620.1 : 543.39 : 579.852.13 Descriptois: Water, quality, water tests, microbiological analysis, micro-organisms, sulfite reducing bacteria, clostridium, filtration analys
2、is English version Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2 : Method by membrane filtration (IS0 6461/2 : 1986) Qualit de leau - Recherche et dnombrement des spores de micro-organismes anarobies sulfito-rducteurs (clostridia) - Parti
3、e 2 : Mthode par filtration sur membrane (IS0 6461/2 : 1986) Wasserbeschaffenheit - Nachweis und Zhlung der Sporen sulfitreduzierender Anaerobier (Clostridien) - %il 2 : Membranfiltrationsverfahren (IS0 6461/2 : 1986) This European Standard was approved by CEN on 1993-01-20. CEN members are bound to
4、 comply with the CENKENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central
5、 Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the
6、 official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. CEN European Committee for Standardization Comit Europe
7、n de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels O 1993 Copyright reserved to CEN members Ref. No. EN 26461-2 : 1993 E Foreword This European Standard is the endorsement of IS0 6461-2. Endorsement of IS0 6461-2 was recommended by lkchnical Co
8、mmittee CEN/TC 230 Water analysis under whose competence this European Standard will henceforth fall. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by July 1993, and conflicting national standards
9、shall be withdrawn at the latest by July 1993. The standard was approved and in accordance with the CENKENELEC Internal Regulations, the folowing countries are bound to implement this European Standard: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg,
10、 Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, United Kingdom. CEN EN+2b4bL-2 93 3404589 0042977 462 Page 3 EN 26461-2 : 1992 Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2: Method by membrane filtration O Introduction The spo
11、res of sulfite-reducing anaerobes (clostridia) are wide- spread in the environment. They are present in human and animal faecal matter, in waste water and in soil. Unlike Escherichla cdi and other coliform organisms, the spores sur- vive in water for long periods as they are more resistant than vege
12、tative forms to the action of chemical and physical factors. They may thus give an indication of remote or intermittent pollution. They may even be resistant to chlorination at levels which are normally used for the treatment of water, and they are thus useful for control purposes. IS0 6461 consists
13、 of the following parts : Part 1 : Method by enrichment in a liquid medium. Part 2: Method by membrane filtration. 1 Scope This part of IS0 6461 specifies a method for the detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) by membrane filtration. 2 Field of applicatio
14、n The method can be applied to all types of water, except when a large amount of particulate material is liable to be retained by the membrane. . 3 References IS0 3696, Water for laboratory use - Specfications. IS0 5667, Water quality - Sampling - Part 2: Guidance on sampling techniques. Part3: Guid
15、ance on the preservation and handing of samples. IS0 7704, Water quality - Evaluation of membrane fiters used for microbiological anelyses. ISO8199, Water qualiiy - General guidance for microbiological examination by enumeration of micro- organisms on culture media. ) 4 Definition For the purpose of
16、 this part of IS0 6461, the following defi- nition applies. clostridia : Sulfite-reducing, spore-forming, anaerobic micro- organisms which belong to the Bacillaceae family and the genus Clostridium. 5 Principle The detection of spores of sulfite-reducing anaerobes (clostridia) in a specified volume
17、of a water sample requires the following steps. 5.1 Selection of spores Selection of spores in the sample by applying heat for a period of time sufficient to destroy vegetative bacteria. 5.2 Membrane filtration and culture Filtration of the water sample through a membrane filter having a pore size s
18、uch that bacterial spores (0.2 pm) are retained in or on the membrane filter. Placing of the filter on a specialized selective culture medium (sulfite-iron-agar), followed by incubation at 37 f 1 OC for 20 f 4 h and 44 k 4 h, and counting of any black colonies. 6 Culture media and reagents 6.1 Basic
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