EN 16421-2014 en Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)《人类生活用水中含有物质的影响 微生物生长的增加(EMG)》.pdf
《EN 16421-2014 en Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)《人类生活用水中含有物质的影响 微生物生长的增加(EMG)》.pdf》由会员分享,可在线阅读,更多相关《EN 16421-2014 en Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)《人类生活用水中含有物质的影响 微生物生长的增加(EMG)》.pdf(70页珍藏版)》请在麦多课文档分享上搜索。
1、BSI Standards PublicationBS EN 16421:2014Influence of materials on waterfor human consumption Enhancement of microbialgrowth (EMG)BS EN 16421:2014Incorporating corrigendum December 2015BS EN 16421:2014 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN 16421:2014.T
2、he purpose of this standard is to describe three European test methods that can be applied to determine the ability of nonmetallic materials to enhance microbial growth in drinking water.Each method uses different performance characteristics, which allows its use for specific materials or product ty
3、pes, but all three methods have limitations. For example, multi-layer pipes cannot currently be tested with the Method 1 and Method 3, and small components cannot easily be analysed using Method 2. Harmonized product standards will specify the particular method to follow, taking into account materia
4、l of construction and type of components. Pass/fail criteria is not included in this standard and will be specified by the individual Member State.At the time of publication, this standard is not currently included in the (UK) product testing to be used for materials in contact with drinking water a
5、s all the methodology in BS 6920, together with the pass/fail criteria, has not been replaced by European Standards. Studies to establish the degree of agreement of the results obtained using the three methods on a wide range of specific materials types will enable the establishment of comparative p
6、ass/fail criteria. To ensure the correct methodology is being used, refer to the National Approval schemes for the UK before undertaking any testing. Further information can be obtained from UK approvals bodies, such as the Drinking Water Inspectorate and the Water Regulations Advisory Scheme.The UK
7、 participation in its preparation was entrusted to Technical Committee EH/6, Effects of materials on water quality.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract
8、. Users are responsible for its correct application. The British Standards Institution 2015. Published by BSI Standards Limited 2015ISBN 978 0 580 92629 7ICS 07.100.20Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the author
9、ity of the Standards Policy and Strategy Committee on 31 January 2015.Amendments/corrigenda issued since publicationDate Text affected31 December 2015 Implementation of CEN Correction Notice 03 February 2015: Subclauses 5.5.2.2 and 5.6.9 modifiedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 1
10、6421 December 2014 ICS 07.100.20 English Version Influence of materials on water for human consumption - Enhancement of microbial growth (EMG) Influence des matriaux sur leau destine la consommation humaine - Stimulation de la croissance microbienne (SCM) Einfluss von Materialien auf Wasser fr den m
11、enschlichen Gebrauch - Frderung des mikrobiellen Wachstums This European Standard was approved by CEN on 25 October 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard witho
12、ut any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other l
13、anguage made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Den
14、mark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTE
15、E FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16421:2014 EEN 16421:2014 (E)
16、 2 Contents Page Foreword 6 Introduction .7 1 Scope 9 2 Normative references 9 3 Terms and definitions 10 4 Influence of materials on water for human consumption Enhancement of microbial growth (EMG) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP 11 4.1 General . 11 4.2 Pri
17、nciple . 12 4.3 Apparatus 12 4.3.1 Requirements 12 4.3.2 Vessels, containers, stoppers and connectors and general laboratory apparatus . 12 4.3.3 Cleaning . 12 4.3.4 Gloves 12 4.3.5 Plates . 12 4.3.6 Test containers . 13 4.3.7 Test tubes 13 4.3.8 Incubator (or hot room) 13 4.3.9 Sterilisation ovens
18、13 4.3.10 Membrane filters . 13 4.3.11 Autoclave . 13 4.4 Reagents 13 4.4.1 Test water 13 4.4.2 Dilution water 14 4.4.3 Potassium dihydrogen orthophosphate solution . 14 4.4.4 Potassium-nitrate solution 14 4.4.5 Sodium acetate solution 15 4.4.6 Inoculum 15 4.4.7 Specifications for controls 15 4.5 Te
19、st samples and controls 16 4.5.1 Sampling, transport and storage of test samples . 16 4.5.2 Test piece preparation . 16 4.6 Pre-treatment of test pieces 17 4.6.1 General . 17 4.6.2 Flushing . 17 4.6.3 Stagnation . 17 4.6.4 Prewashing 17 4.7 Test procedure 17 4.7.1 Overview 17 4.7.2 ATP contamination
20、 . 18 4.7.3 Preparation of the test container 18 4.7.4 Test pieces 18 4.7.5 Incubation 18 4.7.6 Positive and negative controls 18 4.7.7 Changing of test water . 19 4.7.8 Measurement of ATP concentrations . 19 4.8 Calculation of test results 19 4.8.1 Biomass Production (BP) 19 4.9 Reproducibility and
21、 repeatability 22 BS EN 16421:2014EN 16421:2014 (E) 2 Contents Page Foreword 6 Introduction .7 1 Scope 9 2 Normative references 9 3 Terms and definitions 10 4 Influence of materials on water for human consumption Enhancement of microbial growth (EMG) Method 1: Measured by Biomass Production Potentia
22、l (BPP) measured by ATP 11 4.1 General . 11 4.2 Principle . 12 4.3 Apparatus 12 4.3.1 Requirements 12 4.3.2 Vessels, containers, stoppers and connectors and general laboratory apparatus . 12 4.3.3 Cleaning . 12 4.3.4 Gloves 12 4.3.5 Plates . 12 4.3.6 Test containers . 13 4.3.7 Test tubes 13 4.3.8 In
23、cubator (or hot room) 13 4.3.9 Sterilisation ovens 13 4.3.10 Membrane filters . 13 4.3.11 Autoclave . 13 4.4 Reagents 13 4.4.1 Test water 13 4.4.2 Dilution water 14 4.4.3 Potassium dihydrogen orthophosphate solution . 14 4.4.4 Potassium-nitrate solution 14 4.4.5 Sodium acetate solution 15 4.4.6 Inoc
24、ulum 15 4.4.7 Specifications for controls 15 4.5 Test samples and controls 16 4.5.1 Sampling, transport and storage of test samples . 16 4.5.2 Test piece preparation . 16 4.6 Pre-treatment of test pieces 17 4.6.1 General . 17 4.6.2 Flushing . 17 4.6.3 Stagnation . 17 4.6.4 Prewashing 17 4.7 Test pro
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