BS EN ISO 21148-2009 Cosmetics - Microbiology - General instructions for microbiological examination《化妆品 微生物学 微生物检查的一般说明书》.pdf
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1、Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 21/04/2010 03:05, Uncontrolled Copy, (c) BSIg49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58f
2、or microbiological examinationICS 07.100.99; 71.100.70Cosmetics Microbiology General instructions BRITISH STANDARDBS ISO EN21148:2009BS EN ISO 21148:2009Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 21/04/2010 03:05, Uncontrolled Copy, (c) BSIThis British Standard was published under the au
3、thority of the Standards Policy and Strategy Committee on 6 January 2006 BSI 2010ISBN 978 0 580 66831 9Amendments/corrigenda issued since publicationDate Comments 31 January 2010 This corrigendum renumbers BS ISO 21148:2005 as BS EN ISO 21148:2009This publication does not purport to include all the
4、necessary provisions of a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunity from legal obligations.National forewordThis British Standard is the UK implementation of EN ISO 21148:2009. It is identical to ISO 21148:2005. It supersede
5、s BS ISO 21148:2005 which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee CW/217, Cosmetics.A list of organizations represented on this committee can be obtained on request to its secretary.EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 21148 June
6、2009 ICS 07.100.99; 71.100.70 English Version Cosmetics - Microbiology - General instructions for microbiological examination (ISO 21148:2005) Cosmtiques - Microbiologie - Instructions gnrales pour les examens microbiologiques (ISO 21148:2005, Cor 1:2006 inclus) Kosmetik - Mikrobiologie - Allgemeine
7、 Anleitungen fr mikrobiologische Untersuchungen (ISO 21148:2005) This European Standard was approved by CEN on 23 May 2009. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard wit
8、hout any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other languag
9、e made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
10、 France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMIT
11、EE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 21148:2009: ELicensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 21/04/2010 03:05, Uncontrolled Copy, (c
12、) BSIForeword The text of ISO 21148:2005 has been prepared by Technical Committee ISO/TC 217 “Cosmetics” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 21148:2009 by Technical Committee CEN/SS H99 “Products for household and leisure use - Undetermined”
13、the secretariat of which is held by CMC. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2009, and conflicting national standards shall be withdrawn at the latest by December 2009. Attent
14、ion is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of t
15、he following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovak
16、ia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 21148:2005 has been approved by CEN as a EN ISO 21148:2009 without any modification. ii BS EN ISO 21148:2009BS ISO 21148:2005Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 21/04/2010 03:05, Un
17、controlled Copy, (c) BSIContents Page2 5 7 8 9 10 11 12 Annex A (informative) Basic identification techniques 14Neutralization of the antimicrobial properties of the product . 13Expression of results 13Operating practices . 12Laboratory samples 10Annex B (informative) Basic techniques for counting a
18、nd plating . 19Annex C (informative) Preparation and calibration of inoculums . 20Preparation and sterilization of culture media and reagents 8Preparation of the apparatus and glassware 66 Personnel . 6Strains of microorganisms . 64 Equipment 33 Premises . 1Terms and definitions 11 Scope 1iii Introd
19、uction . ivBS EN ISO 21148:2009BS ISO 21148:2005Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 21/04/2010 03:05, Uncontrolled Copy, (c) BSIIntroduction The purpose of this International Standard is to help ensure that the general techniques used for conducting cosmetic microbiological examin
20、ations are the same in other laboratories that adopt these standards, to help achieve homogeneous results in different laboratories and to contribute towards the protection of the health of the laboratory personnel by preventing risk of infection. When conducting microbiological examinations for cos
21、metic products, it is especially important that: only those microorganisms which are present in the samples be isolated or enumerated; the microorganisms do not contaminate the environment. In order to achieve this, it is necessary to pay attention to personal hygiene and to use working techniques w
22、hich ensure, as far as possible, exclusion of extraneous contamination. Since, in this International Standard it is possible to give only a few examples of the precautions to be taken during microbiological examinations, a thorough knowledge of the microbiological techniques and of the microorganism
23、s involved is essential. It is important that the analyses be conducted as accurately as possible, including calculation of the number of microorganisms. A large number of manipulations can, for example, unintentionally lead to cross-contamination and the analyst should always verify the accuracy of
24、 the results given by his/her technique. It is necessary to take special precautions, not only for reasons of hygiene, but also to ensure good reproducibility of the results. It is not possible to specify all the precautions to be taken in all circumstances, but this International Standard at least
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