ASTM E2362-2009 Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection《硬表面消毒用预饱和或浸渍小毛巾的评估的标准实施规程》.pdf
《ASTM E2362-2009 Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection《硬表面消毒用预饱和或浸渍小毛巾的评估的标准实施规程》.pdf》由会员分享,可在线阅读,更多相关《ASTM E2362-2009 Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection《硬表面消毒用预饱和或浸渍小毛巾的评估的标准实施规程》.pdf(5页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: E2362 09Standard Practice forEvaluation of Pre-saturated or Impregnated Towelettes forHard Surface Disinfection1This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision,
2、 the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This practice is designed to evaluate the antimicrobialactivity of pre-saturated or impregnated towelette
3、s when usedas a hard surface disinfectant.1.2 It is the responsibility of the investigator to determinewhether Good Laboratory Practices (GLPs) are required andto follow them when appropriate.1.3 This practice should be performed only by those trainedin microbiological techniques.1.4 The values stat
4、ed in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and
5、 health practices and to determine theapplicability of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE1054 Test Methods for Evaluation of Inactivators of An-timicrobial Agents2.2 Federal Standard40 CFR, Part 160 Good Laboratory Pr
6、actice Standards33. Terminology3.1 carrier, na transportable surface onto which a testorganism will be inoculated and dried. The carrier will betreated with the test substance and subcultured for survivors.3.2 CFU, ncolony forming units3.3 disinfectant, na physical or chemical agent or processthat d
7、estroys pathogenic or potentially pathogenic microorgan-isms in/on surfaces or objects.3.4 impregnated, adjsaturated with test substance.3.5 neutralizer, na component used to render an activeagent incapable of destroying organisms by chemical orphysical means.3.6 pre-saturated, adjto be filled or im
8、pregnated with testsubstance prior to the time of its intended use.3.7 towelette, nA paper, cloth or non-woven blend mate-rial used as a transporter for a cleaning and/or disinfectionagent.4. Summary of Practice44.1 A towelette impregnated or pre-saturated with a testsubstance is used to treat a car
9、rier which has been inoculatedwith a test organism after an aliquot of a test organism has beeninoculated, evenly distributed, and dried onto the carrier. Thecarrier is wiped using the pre-saturated or impregnated tow-elette simulating the application of the test substance and thenheld for a pre-det
10、ermined contact time. After the specifiedcontact time, the test substance remaining on the carrier isneutralized and the carrier is subcultured to recover survivingtest organism. The used towelette, after the contact time, is alsocultured for surviving test organism.5. Significance and UseThis test
11、method may be used to determine if a pre-saturatedor impregnated towelette demonstrates antimicrobial effective-ness as a disinfectant on hard surfaces.6. Apparatus6.1 Incubatorany calibrated incubator that maintains atemperature specific for propagation of organisms. (for ex-ample, bacteria and myc
12、obacteria at 35 6 2 C and fungi at25 6 2 C).1This practice is under the jurisdiction of ASTM Committee E35 on Pesticidesand Alternative Control Agents and is the direct responsibility of SubcommiteeE35.15 on Antimicrobial Agents.Current edition approved Dec. 1, 2009 Published December 2009. Original
13、lyapproved in 2004. Last previous edition approved in 2004 as E2362 04. DOI:10.1520/E2362-09.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document
14、Summary page onthe ASTM website.3Available from the Superintendent of Documents, U.S. Government PrintingOffice, Washington D.C. 204024United States Environmental Protection Agency, Efficacy Data Requirements,“Pre-Saturated or Impregnated Towelettes for Hard Surface Disinfection” StandardOperating P
15、rocedure for Testing of Towelette Disinfectants against Staphylococcusaureus and Pseudomonas aeruginosa, EPA/OPP Microbiology Laboratory, Ft.Meade, MD. SOP# MB09-02, Revised 12/31/06.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.6.
16、2 Sterilizerany suitable steam sterilizer that producesthe conditions of sterilization is acceptable.6.3 Test Toweletteswith instructions for use.6.4 Timer (Stop-clock)a calibrated timer that displays minand s.6.5 Spectrophotometercalibrated to 650 nm.6.6 Mixera vortex mixer is recommended.6.7 pH me
17、tera calibrated pH meter to determine the pH ofmedia.6.8 Nonporous Test Carriersborosilicate glass slides, 253 75 3 2 mm slides, pre-cleaned (or other hard surfaces andsizes as appropriate).6.9 Glass Culture Tubes20 3 150 or 25 3 150 mmwithout lip or equivalent.6.10 Culture Tube Closuresappropriate
18、size nontoxic clo-sures.6.11 Petri Dishes100 3 15 mm, glass and plastic, sterile.6.12 Balancea calibrated balance sensitive to 0.1 g.6.13 Micropipettorcalibrated for dispensing 10 L.6.14 Forcepssterilizable forceps.6.15 Sterilizer Apparatusa bunsen burner or other appro-priate heat sterilizer.6.16 B
19、acteriological Culture Loop 4 mm inside diameterloop of platinum or platinum alloy wire or sterile disposableplastic loops of appropriate size.6.17 Colony Counterany one of several types may beused, for example Quebec.6.18 Glovessterile gloves not possessing antimicrobialproperties.6.19 Pipettesteri
20、le volumetric pipettes.6.20 Glass Jars100 mL or other appropriate vessel.6.21 Filter Paper9 cm (Whatman No. 2, or equivalent)sterilized prior to use.6.22 Thermometercalibrated thermometer.6.23 Glass Beads3 5 mm sterile beads.6.24 Gauzesterile cotton gauze.6.25 Hemacytometercalibrated hemacytometer.6
21、.26 Glass Woolsterile grease free glass wool.6.27 Hot air ovenability to maintain 180C.6.28 Tissue grindersterile disposable or sterilizable glass.6.29 Orbital Shakercalibrated shaker.7. Reagents7.1 Culture MediaBacteria7.1.1 Nutrient BrothPseudomonas aeruginosa,7.1.2 Cystine Trypticase AgarPseudomo
22、nas aeruginosa,7.1.3 Synthetic BrothSalmonella enterica and Staphylo-coccus aureus.7.1.4 Nutrient Agar.7.1.5 Fluid Thioglycollate Broth.7.2 Culture MediaMycobacteria7.2.1 Middlebrook 7H11 or 7H9 Agar Slants.7.2.2 Modified Proskauer-Beck Broth.7.3 Culture MediaFungi7.3.1 Sabouraud Dextrose Agar plate
23、s/Potato DextroseAgar.7.3.2 Sabouraud Dextrose Agar slants.7.4 Neutralizing Subculture MediaA neutralizing growthmedium capable of supporting the growth of the test organismfollowing exposure to the test material in accordance withE1054.7.5 Subculture Agar7.5.1 Tryptic Soy Agar with or without sheep
24、 bloodBacteria.7.5.2 Middlebrook 7H11 AgarMycobacteria.7.5.3 Sabouraud Dextrose AgarFungi.7.6 Other subculture agars, broths and neutralizers may beused where appropriate.7.7 SoilBlood Serum, such as heat inactivated fetal bo-vine serum or other appropriate alternative soil.7.8 Dilution Fluidsterile
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