ARMY MIL-D-51503-1984 DETECTOR TICKET ANTICHOLINESTERASE AGENT EEL ENZYME《机票探测器 鳗鱼酶抗胆碱酯酶剂》.pdf

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1、MIL-D-51503 bb 77777Ob OY231b2 4 U MIL-D-51503 (EA) (g) 12 October 1984 (See section 6) *-.J MILITARY SPECIFICATION DETECTOR TICKET, ANTICHOLINESTERASE AGENT, EEL ENZYME This specification is approved for use by US Army Armament, Munitions and Chemical Command, Department of the Army, and is availab

2、le for use by all Depart- ments and Agencies of the Department of Defense. II SCOPE 1,l Scope. This specification covers to detect anticholinesterase agents such one type of detector ticket designed as GB and VX. 2. APPLICABLE WCUMENTS 2.1 Government documents. 2.1.1 Specifications, standards, and h

3、andbooks. Unless otherwise specified, the following specif icatlons , standards, and handbooks of the issue listed in that issue of the Department of Defense Index of Specifications and Standards (DoDISS) specified in the solicitation form a part of this specification to the extent specified herein.

4、 STANDARDS MILITARY MIL-STD-105 - Sampling Procedures and Tables for Inspection by Attributes 2.1.2 Other Government documents, drawings, and publications. The following other Government documents, drawings, and publications form a part of this spec- JficatLon to the extent specified herein. : Benef

5、icial comments (recommendations, additions, deletions) and any perti- : : nent data which may be of use in improving this document should be addressed: : to: Commander, Armament Research and Development Center, US Amy Armament, : : Munikions and Chemical Command, ATTN: : Ground, MD 21010-5423 by usi

6、ng the self-addressed Standardization Document : : Improvement Proposal (DD Form 1426) appearing at the end of this document : : or by letter. FSC 6665 DRSMC-TSC-S( A) , Aberdeen Proving Provided by IHSNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-MIL-D-51503 bb

7、I 777990b 0423163 O I MIL-D-51503 ( EA) DRAW1 NGS US AR!Y ARMAMENT, MUNITIONS AND CHEMICAL COMMAND CHEMICAL RESEARCH ANI) DEVELOPMENT CENTER 5-77-2510 - Ticket Packet Assembly 5-77-2528 - Clip (Copies of specifications, standards, handbooks, drawings and publications required by manufacturers in con

8、nection with specific acquisition functions should be obtained from the contracting activity or as directed by the contrac- ting officer.) 2*2 Other publications. The following documents form a part o% this speci- ficntion to the extent specified herein. indicated as Do? adopted shall be the issue l

9、isted in the current DoDISS and the supplement thereto, if applicable. The issues of the documents which are ASP4 STAHDARDS D 1193 - Reagent Water (Application for copies should be addressed to ASTM, 1916 Race Street, Philadelphia, PA 19103 . ) ( Industry association specifications and standards are

10、 generally available for reference from libraries. and using Federal agencies.) They are also distributed among technical groups Munsell Book of Color (The Munsell Book of Color may be obtained from the Munsell Color Company, Inc., 2441 No Calvert Street, Baltimore, MD 21218.) 2.3 Order of precedenc

11、e. this specification and the references cited herein, the text of this specifica- tion shall take precedence. In the event of a conflict between the text of 3. REQUIREMENTS 3.1 Materials and components. 3.1.1 Materials. Al1 materials cited on Drawing 5-77-2510 and subsidiary drawings shall-conform

12、to the specifications listed thereon or to the specific characteristics set forth on the drawings. 2 Provided by IHSNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-I MIL-D-51503 bb W 777770b 0423Lb4 2 MIL-D-51503 ( W) 3.1.2 Components. All components of the detecto

13、r ticket packet assembly shall conform to Drawing 5-77-2510 and subsidiary drawings . 3.2 Manufacture and assembly. The detector ticket packet shall be manu- factured and assembled in accordance with Drawing 5-77-2510 and subsidiary drawings . 3.3 Perforaance requirements for ambient stored detector

14、 tickets. 3.3.1 Cholinesterase recovery before aging. No less than 0.20 and no more than 0.80 cholinesterase units Ber disc shall be recovered before aging when tested as specified in 4.4.4.1, 3.3.2 VX sensitivity before aging. Detector tickets shall have no color change before aging when tested as

15、specified in 4.4.4.2, 3.3.3 Blank before aging. After 3 minutes, upon challenging with pure water hue 7.5BG, lOBG, 2.5B, 5B, before aging, the enzyme disc (clipped end) shall develop one of the following Munsell colors when tested as specified in 4.4.4.3: 7.5B, 103; value 7 to 9; chroma 2 or darker.

16、 3.4 Performance requirements for accelerated aging conditions . The detector ticket shall meet the following requirements after accelerated aging for 9 con- tinuous days at 90 - + 2OC. 3.4.1 Cholinesterase recovery after aging. After accelerated aging, no less than 0.20 and no more than 0.80 cholin

17、esterase units per disc shall be recov- - ered when tested as specified in 4.4.4.4. 3.4.2 VX sensitivity after aging. Detector tickets shall have no color change after accelerated aging when tested as specified in 4.4.4.5. 3.4.3 Blank after aging. After 3 minutes, upon challenging with pure water af

18、ter accelerated aging, the enzyme disc (clipped end) shall develop one of the following Munsell colors when tested as specified in 4.4.4.6: hue 7.5BG, lOBG, 2.5B or 5B; value 7 to 9; chroma 2 or darker. 3.5 pH of water extract. Detector tickets shall have a pH between 8.5 and that of the water befor

19、e extraction when tested as specified in 4.4.4.7. 3.6 Tear opening. Detector tickets shall be removable from the foil pack without distorting the ticket or using a tool or finger to enlarge the opening when tested as specified in 4.4.4.8. 3.7 Leakage. There shall be no visible bubble leakage any pla

20、ce on the tickets foil pack whentested as specified in 48484.90 3.8 Overlay heat seal. The overlay shall strip off so that no more than 1/16 inch of overlay material extends inside the inner edge of the heat seal when tested as specified in b.4.4.10. 3 Provided by IHSNot for ResaleNo reproduction or

21、 networking permitted without license from IHS-,-,-3.9 Workmanship. The detector tickets and packages shall be free from tears, rips, punctures, cracks, dirt, grease, oil, and poor heat seals. 3.10 Preproduction. Prior to the start of regular production, a preproduc- Regular production techniques, m

22、aterials, and equipment shall be used tion lot of detector tickets shall be produced in accordance with this specifi- cation. to produce the preproduction lot. reagent, or inaterial shall be used for the preprodirction lot. No more than one lot of each component, 4 . QUALITY ASSURANCE PROVISIONS 4.1

23、 Responsibility for inspection. Unless otherwise specified in the con- tract or purchase order, the contractor is responsible for the performance of all inspection requirements as specified herein. in the contract or purchase order, the contractor ;a4y use his own or any other facilities suitable fo

24、r the performance of the inspection requirements speci- fied herein, unless disapproved by the Government. The Government reserves the right to perform any of the inspections set forth in the specification where such inspections are deemed necessary to assure supplies and services conform to prescri

25、bed requirements. Except as otherwise specified 4.2 Classification of inspections. The inspection requirenents specified herein are classified as follows: (a) Preproduction inspection (see 4.3) (b) Quality conformance inspection (see 4.4) 4.3 Preproduction inspection. 4.3.1 Lotting. A preproduction

26、lot of no less than 525 detector tickets shall be manufactured using the same methods, materials, equipment, and pro- cesses of manufacture as will be used during regular production (see 6.2). 4 3 .2 Sampling and inspection procedure . 4.3.2.1 For examination. Sampling and inspection procedure for e

27、xamination shall be as specified in 4.4.2.1 and 4.4.3.1. 4.3.2.2 For test. Sampling shall be conducted in accordance with table I. Tests shall be conducted as specified in 4.4.4. 4 . 4 Quality conformance inspect ion . 4.4.1 Lotting. A lot of tickets shall consist of the detector tickets impregnated

28、 with one batch of eel enzyme (see 6.3). 4 Provided by IHSNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-i MIL-D-51503 66 m 7797706 0423Lhh h MIL-D-51503 ( EA) TABLE I, Preproduction and quality conformance. inspection . : Accept/reject : Test I Requirements Sampl

29、e size criteria : paragraph : : Cholinesterase recovery : MIL-STD-105, : AQL 2.5 percent : : before aging : inspection level S-3 : defect ive . . : YX sensitivity before t MIL-STD-105, : AQ,L 2-5 percent : : aging : inspection level S-3 : defective : Blank before aging MIL-STD-105, : AQL 2.5 percent

30、 : I I . : inspection level 8-3 : defective I : cholinesterase recovery : MIL-STD-105, : AQL 2.5 percent : : after aging : inspection level S-3 : defect ive : VX sensitivity after aging : MIL-STD-105, : AQL 2.5 percent : . : inspection level S-3 : defective . . : Blank after aging . MIL-STD-105, : A

31、QL 2.5 percent : : inspection level S-3 : defective * . . * e. : pH of water extract : 3.6 tickets per lot : acc O, rej 1 : : Tear test MIL-STD-105, : AQL 2.5 percent : * : inspection level S-3 : defect ive : Leakage MIL-STD-105, : AQL 2.5 percent : . : inspection level S-3 : defect ive : Overlay he

32、at seal MIL-STD-105, : AQL 2.5 percent : : inspection level S-3 : defect ive . . I . . 4.4.4.i : 4.4.4.2 : 4.4.4.3 : 4.4.4.4 : 4.4.4-5 : 404,406 : . 4.4.4,7 : 4.4.4.8 : 4.4.4.9 : 4.4.4.10 : 4 . 4 2 Sampling . 4,4.2.1 For examination. Sampling for examination shall be conducted in accordance with MIL

33、-STD-105. 4.4,2.2 For test. Sampling for test shall be conducted in accordance with MIL-STD-105 and table I, 4 , 4 , 3 4.4,3,1 For examination. Inspect ion procedure . The sample unit shall be one detector ticket, Sample detector tickets shall be examined in accordance with MIL-STD-105 and the follo

34、wing classification of defects: 5 Provided by IHSNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-MIL-D-53503 bb 779770b 0423367 = (a) Ticket packet assembly (Drawing 5-77-2510) . Categories Defects Critical: None defined Major: AQL 1.0 percent defective 101 102 Com

35、ponents missing or incorrectly installed Foil pouch sealed area torn, punctured or shows trapped bubbles (this examination must be done prior to assembly of the label) 103 104 Lot number nissing Pouch length or width incorrect *lo5 Substrate disc, enzyme disc, or overlay heat seal incorrect 106 107

36、Workinanship (see 3.9) Tear notches too deep or missing Minor: AQL 2.5 percent defective 201 202 Label missing or incorrect 203 Position of enzyme or substrate disc incorrect 204 Score line missing Pouch seals less than 3/16 inch *May be inspected prior to assembly of the ticket into the pouch. 4.4.

37、3.2 For test. 4.4.4 Tests (see 6.7). Inspection procedure shall be as specified in table I. Water in accordance with ASm Di193 and reagent grade chemicals shall be used throughout the tests. Where applicable, blank dete.minations shall be run and corrections applied where significant. Tests shall be

38、 conducted as follows: 4.4.4.i Cholinesterase recovery before aging. (a) PH stat solution. To prepare 1 liter of pH stat solution, dissolve 8.120 grams (g) of magnesium chloride (O.O4M), 5.850 g of sodium chloride (O.lOM), and 0.730 Q of acetylcholine chloryde or 0.909 g of acetyl- choline bromide i

39、n 900 milliliters (mL) of deionized water having a pH between 5.0 and 6.0 (the pH of final solution will be approximately 4.5). Adjust the final volume to 1 liter with deionized water. (Solution is stable for 10 con- secutive calendar days if kept refrigerated at 5“ - + 5C.) 6 Provided by IHSNot for

40、 ResaleNo reproduction or networking permitted without license from IHS-,-,-1 MIL-D-53503 bb 7977706 0423368 T MIL-D-51503 ( EA) i (b) Procedure, To a 100-mL beaker add 50 mL of pH stat solution prepared as specified in (a). Equilibrate this solution in a water bath to 37“ + 1C. To a 20-mL beaker ad

41、d 2.0 mL of deionized water having a pH between 7.0 and 7.2. Peel off one eel acetylcholinesterase enzyme-impregnated paper disc from the clipped end of the ticket and add to the beaker. (Scrape off as much of disc as possible from around seal area and also add these scrapings to the beaker.) Break

42、up the paper disc with a glass rod and then stir vigorously at room temperature for 5 minutes with a magnetic stirrer. While this is mixing, adjust the pH of the pH stat solution to 8.0 using an automatic, titrator (pH stat type), using 0.004N NaOH and maintaining 37“ + 1C. After tenperature and pH

43、have equilibrated, let the solution stir an addTtional 5 minutes while main- taining a pH of 8.0, Pour approximately i5 mL of pH 8.0 pH stat solution into the 20-mL beaker with enzyme disc and then quickly transfer the mixture back into the 100-mL titra- tion beaker which is placed back into the 37C

44、 bath. Start the timer immedi- Fztely upan mixing. automatic titrator will titrate to and control the pH at 8.0. titration is running, record the buret reading at 2 minutes. stop the titration and record the final buret reading. esterase units per disc recoverd as follows: - Record the volume of NaO

45、H added as the blank. Record the buret reading and start titration. The While the After 7 minutes, Calculate %he cholin- 1000 (A - D) B c Recovered units per disc = where: A = Milliliters of NaOH used between 2 and 7 minutes, B = Normality of NaOH, C = Time in niinutes, and D = Milliliters of NaOH u

46、sed during the 5 minute blank period. 4.4.4.2 VX sensitivity before aging. (a) Preparation of VX agent stock solutions. Dilute a quantity of VX of known purity, based on an assay performed within the last 60 days (see 6.6 for a suggested method), to 100 mL in a volumetric flask with isopropyl alcoho

47、l so that the solution contains 10 microliters of pure VX (Stock Solution A). Dilute 0.1 mL of Stock Solution A to 100 mL in a volumetric flask with deionized water having a pH of between 5.0 and 6.0 (Stock Solution B). 2 mL of Stock Solution B to 10 mL with deionized water in a volumetric flask to

48、produce a working solution of 0.02 milligrams (mg) VX per liter. tions shall not be more than seven consecutive calendar days old when used. The pH of the deionized water may be adjusted with hydrochloric or sulfuric acid if necessary. Dilute Stock soh- (b) Ticket agent VX testing procedure. Wet a n

49、ew cnzyine disc (clipped end) by dipping it in 65“ to 75F VX working solution for 5 seconds. If the enzyme disc does not completely wet, replace the foil overlay and lightly press the area over the enzyme disc to saturate the entire surface. More VX working solution can be added if necessary. completely wet, fold the silver cover back over the patch and fold the ticket. After the enzyine disc is 7 Provided by IHSNot for ResaleNo reproduction or net