BS ISO 18337-2015 Surface chemical analysis Surface characterization Measurement of the lateral resolution of a confocal fluorescence microscope《表面化学分析 表面特征 共焦荧光显微镜横向分辨率的测量》.pdf
《BS ISO 18337-2015 Surface chemical analysis Surface characterization Measurement of the lateral resolution of a confocal fluorescence microscope《表面化学分析 表面特征 共焦荧光显微镜横向分辨率的测量》.pdf》由会员分享,可在线阅读,更多相关《BS ISO 18337-2015 Surface chemical analysis Surface characterization Measurement of the lateral resolution of a confocal fluorescence microscope《表面化学分析 表面特征 共焦荧光显微镜横向分辨率的测量》.pdf(22页珍藏版)》请在麦多课文档分享上搜索。
1、BSI Standards PublicationBS ISO 18337:2015Surface chemical analysis Surface characterization Measurement of the lateralresolution of a confocalfluorescence microscopeBS ISO 18337:2015 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 18337:2015.The UK participati
2、on in its preparation was entrusted to TechnicalCommittee CII/60, Surface chemical analysis.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible f
3、or its correctapplication. The British Standards Institution 2015. Published by BSI StandardsLimited 2015ISBN 978 0 580 81132 6ICS 71.040.40Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy a
4、nd Strategy Committee on 30 June 2015.Amendments issued since publicationDate Text affectedBS ISO 18337:2015 ISO 2015Surface chemical analysis Surface characterization Measurement of the lateral resolution of a confocal fluorescence microscopeAnalyse chimique des surfaces Caractrisation des surfaces
5、 Mesurage de la rsolution latrale dun microscope confocal fluorescenceINTERNATIONAL STANDARDISO18337First edition2015-06-15Reference numberISO 18337:2015(E)BS ISO 18337:2015ISO 18337:2015(E)ii ISO 2015 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2015, Published in SwitzerlandAll rights reser
6、ved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
7、 at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 18337:2015ISO 18337:2015(E)Foreword ivIntroduction v1 Scope . 12 Term
8、s and definitions . 13 Symbols and abbreviated terms . 14 General 14.1 Background information 14.2 Types of CFM operation 24.2.1 General 24.2.2 Stage scanning CFM . 24.2.3 Laser scanning CFM 24.2.4 Spinning disk CFM 24.3 Parameters that affect the lateral resolution of a CFM . 34.3.1 General 34.3.2
9、Objective lens 34.3.3 Detection pinhole size and focal length of the tube lens . 34.3.4 Collimation and purity of the laser illumination beam 34.3.5 Polarization of the laser illumination 34.3.6 Excitation and emission wavelengths 34.3.7 Image contrast . 35 Measuring the lateral resolution by imagin
10、g a small object . 35.1 Background information 35.2 Selection of the sample and sample requirements 45.3 Setting the parameters prior to operating the instrument . 45.4 Data collection and analysis 55.4.1 Selecting a proper spot . 55.4.2 Extracting a line profile using band-average process 55.5 Reco
11、rding the data . 6Annex A (informative) Sample preparation and example of data and analysis . 7Bibliography .10 ISO 2015 All rights reserved iiiContents PageBS ISO 18337:2015ISO 18337:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standa
12、rds bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organ
13、izations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its f
14、urther maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/direc
15、tives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the
16、Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related
17、to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 201 Surface chemical analysis.iv ISO 2015 All rights r
18、eservedBS ISO 18337:2015ISO 18337:2015(E)IntroductionConfocal fluorescence microscopes (CFMs) are laser scanning confocal microscopes (LSCMs) operated in a fluorescence imaging mode so as to obtain a fluorescence image of a sample. Fluorescence is the light emitted by a molecule or solid lattice dur
19、ing relaxation after undergoing photon absorption and electronic excitation. The fluorescence wavelength, intensity and spectral shape are specific to the electronic structure of the material; therefore, fluorescence spectroscopy and imaging techniques are useful for chemical characterization and an
20、alysis. Among the optical imaging and spectroscopy tools, CFM yields a high spatial resolution that is advantageous for analysing nanomaterials and thin films. The spatial resolution is one of the most important performance factors for a CFM.The spatial resolution of a technique refers to the maximu
21、m resolvability of two adjacent objects. This value is often characterized in different ways by the manufacturers. The spatial resolution of a CFM is characterized by both the lateral and axial resolution, which have different values and are not necessarily dependent on one another. In this Internat
22、ional Standard, one convenient and effective method for measuring the lateral resolution of a CFM is presented. This method is suitable for use by non-expert operators. ISO 2015 All rights reserved vBS ISO 18337:2015BS ISO 18337:2015Surface chemical analysis Surface characterization Measurement of t
23、he lateral resolution of a confocal fluorescence microscope1 ScopeThis International Standard describes a method for determining the lateral resolution of a confocal fluorescence microscope (CFM) by imaging an object with a size much smaller than the expected resolution.2 Terms and definitionsFor th
24、e purposes of this document, the following terms and definitions apply.2.1point spread functionresponse of an imaging system to a point source or point object2.2lateral resolutiondistance measured either in the plane of the sample surface or in a plane at right angles to the axis of the image formin
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