BS ISO 17410-2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of psychrotrophic micro-organisms《食品和动物饲料的微生物学 适冷微生物计数的水平方法》.pdf
《BS ISO 17410-2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of psychrotrophic micro-organisms《食品和动物饲料的微生物学 适冷微生物计数的水平方法》.pdf》由会员分享,可在线阅读,更多相关《BS ISO 17410-2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of psychrotrophic micro-organisms《食品和动物饲料的微生物学 适冷微生物计数的水平方法》.pdf(14页珍藏版)》请在麦多课文档分享上搜索。
1、BRITISH STANDARD BS ISO 17410:2001 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of psychrotrophic micro-organisms ICS 07.100.30 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBS ISO 17410:2001 This British Standard, having been prepared
2、under the direction of the Consumer Products and Services Sector Committee, was published under the authority of the Standards Committee and comes into effect on 15 July 2001 BSI 07-2001 ISBN 0 580 37544 7 National foreword This British Standard reproduces verbatim ISO 17410:2001 and implements it a
3、s the UK national standard. It supersedes BS 4285-3:Section 3.1:1986 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology, which has the responsibility to: A list of organizations represented on this committee can be obtained on request
4、to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards
5、 Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to u
6、nderstand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comp
7、rises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 7 and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsReference number ISO
8、 17410:2001(E) INTERNATIONAL STANDARD ISO 17410 First edition 2001-05-15 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of psychrotrophic microorganisms Microbiologie des aliments Mthode horizontale pour le dnombrement des micro-organismes psychrotrophesISO 1741
9、0:2001(E) ii ISO 17410:2001(E)iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body int
10、erested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnic
11、al Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publicat
12、ion as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all su
13、ch patent rights. International Standard ISO 17410 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology.INTERNATIONAL STANDARD ISO 17410:2001(E)1 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of psychrotrophic microorgan
14、isms 1 Scope This International Standard specifies a method for the enumeration of psychrotrophic microorganisms by means of the colony-count technique at 6,5 C. The method is applicable to products intended for human consumption or for animal feeding stuffs. 2 Normative references The following nor
15、mative documents contain provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standar
16、d are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standa
17、rds. ISO 6887-1, Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 1: General rules for the preparation of the initial suspension and decimal dilutions. 1) ISO 7218, Microbiology of food and anima
18、l feeding stuffs General rules for microbiological examinations. ISO 8261, Milk and milk products Preparation of samples and dilutions for microbiological examination. 3 Term and definition For the purposes of this International Standard, the following term and definition applies. 3.1 psychrotrophic
19、 microorganism bacteria, yeasts and moulds forming countable colonies under the conditions specified in this International Standard 4P r i n c i p l e 4.1 Two agar plates are prepared using a solid non-selective culture medium, and using a specified quantity of the test sample if the initial product
20、 is liquid, or using a specified quantity of an initial suspension in the case of other products. In order to prevent heat stress, surface inoculation is used. Other pairs of agar plates are prepared, under the same conditions, using decimal dilutions of the test sample or the initial suspension. 1)
21、 Parts 2 to 5 are in preparation, see biliography.ISO 17410:2001(E) 2 4.2 The plates are subjected to aerobic incubation at 6,5 C for 10 days. 4.3 The number of microorganisms per millilitre or per gram of sample is calculated from the number of colonies obtained on the plates chosen. 5 Culture medi
22、a and dilution fluid For current laboratory practice, see ISO 7218. Use only reagents of recognized analytical grade, unless otherwise specified, and distilled or demineralized water or water of equivalent purity. If the prepared culture media and reagents are not used immediately, store them, unles
23、s otherwise stated, in the dark at a temperature of 3 C 2 C for no longer than 1 month, under conditions which do not produce any change in their composition. 5.1 Dilution fluid See ISO 6887-1. 5.2 Plate count agar (PCA) 5.2.1 Composition Enzymatic digest of casein 5,0 g Yeast extract 2,5 g Glucose
24、1,0 g Agar 9gto1 8g a Water 1 000 ml a Depending on the gel strength of the agar. When dairy products are examined, it is recommended to add 1,0 g of skimmed milk powder per litre of the culture medium. The skimmed milk powder shall be free from inhibitory substances. 5.2.2 Preparation Dissolve the
- 1.请仔细阅读文档,确保文档完整性,对于不预览、不比对内容而直接下载带来的问题本站不予受理。
- 2.下载的文档,不会出现我们的网址水印。
- 3、该文档所得收入(下载+内容+预览)归上传者、原创作者;如果您是本文档原作者,请点此认领!既往收益都归您。
下载文档到电脑,查找使用更方便
10000 积分 0人已下载
下载 | 加入VIP,交流精品资源 |
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- BSISO174102001MICROBIOLOGYOFFOODANDANIMALFEEDINGSTUFFSHORIZONTALMETHODFORTHEENUMERATIONOFPSYCHROTROPHICMICROORGANISMS

链接地址:http://www.mydoc123.com/p-585354.html