ASTM E1687-2010(2014) Standard Test Method for Determining Carcinogenic Potential of Virgin Base Oils in Metalworking Fluids《金工流体中原油致癌可能性测定的标准试验方法》.pdf
《ASTM E1687-2010(2014) Standard Test Method for Determining Carcinogenic Potential of Virgin Base Oils in Metalworking Fluids《金工流体中原油致癌可能性测定的标准试验方法》.pdf》由会员分享,可在线阅读,更多相关《ASTM E1687-2010(2014) Standard Test Method for Determining Carcinogenic Potential of Virgin Base Oils in Metalworking Fluids《金工流体中原油致癌可能性测定的标准试验方法》.pdf(9页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: E1687 10 (Reapproved 2014) An American National StandardStandard Test Method forDetermining Carcinogenic Potential of Virgin Base Oils inMetalworking Fluids1This standard is issued under the fixed designation E1687; the number immediately following the designation indicates the year ofo
2、riginal adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers a microbiological test procedurebase
3、d upon the Salmonella mutagenesis assay of Ames et al(1)2(see also Maron et al (2). It can be used as a screeningtechnique to detect the presence of potential dermal carcino-gens in virgin base oils used in the formulation of metalwork-ing oils. Persons who perform this test should be well-versed in
4、the conduct of the Ames test and conversant with the physicaland chemical properties of petroleum products.1.2 The test method is not recommended as the sole testingprocedure for oils which have viscosities less than 18 cSt (90SUS) at 40C, or for formulated metalworking fluids.1.3 The values stated
5、in SI units are to be regarded as thestandard. The values given in parentheses are provided forinformation only.1.4 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate saf
6、ety and health practices and determine the applica-bility of regulatory limitations prior to use. Section 7 providesgeneral guidelines for safe conduct of this test method.2. Referenced Documents2.1 ASTM Standards:3E2148 Guide for Using Documents Related to Metalworkingor Metal Removal Fluid Health
7、and SafetyE2523 Terminology for Metalworking Fluids and Opera-tions2.2 Other Standards:29 CFR 1910.1450 Occupational Exposure to HazardousChemical in Laboratories43. Terminology3.1 Definitions of Terms Specific to This Standard: (See alsoTerminology E2523.)3.1.1 base stock, nthe refined oil componen
8、t of metal-working fluid formulations.3.1.2 PAC (Polycyclic Aromatic Compounds), nFor thepurposes of this test method, PAC refers to fused-ring polycy-clic aromatic compounds with three or more rings. Forexample, the hydrocarbon series is represented by phenan-threne (3), pyrene (4), benzopyrene (5)
9、, dibenzopyrene (6),coronene (7). Heterocyclic polynuclear compounds are alsoincluded in the definition.3.1.3 promutagenic compounds, promutagens,ncompounds that are not directly mutagenic but requiremetabolism for expression of mutagenic activity.3.1.4 Reference Oil 1, nstraight-run naphthenic vacu
10、umdistillate (heavy vacuum gas oil) of known MI and PACcontent recommended for use as a reference standard for themodified Ames test.3.2 Abbreviations:3.2.1 DMSO (Dimethyl Sulfoxide), nextraction agent usedin the preparation of aromatic-enriched oil fractions for muta-genicity testing.3.2.2 G-6-P (G
11、lucose-6-Phosphate), nsubstrate requiredfor the operation of the NADPH generating system involved inthe biological oxidations described above.3.2.3 MI (Mutagenicity Index), nthe slope of the dose-response curve for mutagenicity in the modified Ames test.3.2.3.1 DiscussionMI is an index of relative m
12、utagenicpotency.3.2.4 NADP (Nicotinamide Adenine DinucleotidePhosphate)required cofactor for the biological oxidationsinvolved in activation of PAC to their mutagenic forms.1This test method is under the jurisdiction of ASTM Committee E34 onOccupational Health and Safety and is the direct responsibi
13、lity of SubcommitteeE34.50 on Health and Safety Standards for Metal Working Fluids.Current edition approved Oct. 15, 2014. Published October 2014. Originallyapproved in 1995. Last previous edition approved in 2010 as E1687 - 10. DOI:10.1520/E1687-10R14.2The boldface numbers refer to the list of refe
14、rences at the end of this standard.3For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.4Available from U.S. Gover
15、nment Printing Office Superintendent of Documents,732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http:/www.access.gpo.gov.Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States13.2.5 PAC (Polycyclic Aromatic Compounds), npolyc
16、yclicaromatic compounds.3.2.6 S-9, nfraction prepared from hamster liver whichcontains the enzymes required for metabolic activation ofPACs to their mutagenic forms.4. Summary of Test Method4.1 The Ames Salmonella mutagenicity assay is the mostwidely used short-term in vitro genotoxicity test. The a
17、ssayemploys specific strains of the bacterium Salmonella typhimu-rium that have been mutated at a genetic locus precluding thebiosynthesis of the amino acid histidine which is required forgrowth and reproduction. Additional genetic alterations, someof which are important markers of strain identity,
18、are alsopresent.4.2 The mutagenicity assay relies upon treating the bacteriawith test material over a range of doses immediately below theconcentration showing significant toxicity to the bacteria.Treated bacteria are then grown on agar plates deficient inhistidine. Bacteria possessing the original
19、mutation in thehistidine locus cannot form colonies under these growthconditions, but a certain fraction of treated bacteria which haveundergone a second mutation in the histidine locus revert tohistidine-independence and are able to grow and form visiblecolonies. The number of such revertant coloni
20、es per agar plateis an indicator of the mutagenic potency of the test material.4.3 Typically, the test is conducted using a number ofbacterial strains selectively sensitive to various chemicalclasses of mutagens. Treatment with test compound is carriedout in the presence and absence of a rodent live
21、r extractcapable of mimicking in vivo metabolic activation of promuta-genic compounds (see 3.2 for a listing of terms and abbrevia-tions used). With this combination of test conditions, theAmestest becomes a very effective screening tool for chemicalmutagens. Moreover, because many mutagens are also
22、carcinogens, the test is often used as a screen for carcinogenicpotential.4.4 Although the ability of the Ames test to assess carcino-genic potential is good for many classes of compounds, it hasbeen shown to be generally unsuited to the testing of water-insoluble complex mixtures such as mineral oi
23、ls. To circum-vent poor solubility and other difficulties, this test methodemploys an extraction of the test oil with DMSO to produceaqueous-compatible solutions which readily interact with themetabolic activation system (S-9) and with the tester bacteria.The concentration of S-9 and of NADP cofacto
24、r are increasedrelative to the unmodified assay, and hamster rather than ratliver S-9 is used. The slope of the dose response curve relatingmutagenicity (TA98 revertants per plate) to the dose of extractadded is used as an index of mutagenic potency (MI).4.5 In this test method, the MI (the slope of
- 1.请仔细阅读文档,确保文档完整性,对于不预览、不比对内容而直接下载带来的问题本站不予受理。
- 2.下载的文档,不会出现我们的网址水印。
- 3、该文档所得收入(下载+内容+预览)归上传者、原创作者;如果您是本文档原作者,请点此认领!既往收益都归您。
下载文档到电脑,查找使用更方便
5000 积分 0人已下载
下载 | 加入VIP,交流精品资源 |
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- ASTME168720102014STANDARDTESTMETHODFORDETERMININGCARCINOGENICPOTENTIALOFVIRGINBASEOILSINMETALWORKINGFLUIDS

链接地址:http://www.mydoc123.com/p-529240.html