Annual RefresherCentrifugation Hazards.ppt

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1、Annual Refresher Centrifugation Hazards,Centrifugation 101,Every time you use a centrifuge, you make series of choices. Which centrifuge, which rotor, which tubes and adapters, what speed, for how long, what level of containment.If you are unsure of your choice, ask an experienced colleague, consult

2、 the operating manual, or call the manufacturers representative.,Using Centrifuges,Before placing the rotor in the centrifuge, make sure the bowl is dry and the drive spindle is cleanBalance tubes and bottles carefully and avoid overfilling. If tubes used in fixed angle rotors, centrifugal force dri

3、ves the fluid up the outside tube wall.,Using Centrifuges, continue,Make sure the rotor is seated on the drive hub correctlyDont overload beyond the rotors maximum mass without reducing the rated speedWhen using a horizontal rotor, make sure all buckets are hooked correctly and move freely,Using Cen

4、trifuges, continue,Check O-rings on containers and rotors for cracks, nicks, or chemical attack. Apply vacuum grease to the seals at least weekly.Common user errors are failure to secure the rotor to the drive, failure to put the lid on the rotor, and failure to secure the lid.,Using Centrifuges, co

5、ntinue,It is good practice to stay at the centrifuge until it is running smoothly at the desired run speed.When the run is complete, do not open door until rotor has come to a halt. Always check for possible spill. If you find one, be sure to clean both the centrifuge and rotor thoroughly,Care and L

6、oading of Rotors,Integrity of rotors can be compromised by corrosion or fatigue. Keep rotors clean and dry. Wash immediately if spills occur or if salts or other corrosive materials have been usedBe gentle with aluminum rotors. Avoid harsh detergents or bottle brushes with sharp wire ends.,Care and

7、Loading of Rotors,Inspect rotors regularly. If there are rough spots, pitting, white powder deposits, or heavy discoloration, do not run the rotorMaintain rotor logs. Eventually every rotor must be retired and as ultra rotors age, their maximum speed must be de-rated. It is imperative to keep dilige

8、nt logs.,Centrifuge Explosion Chemical detonation inside an ultracentrifuge,Before,Centrifuge Explosion Chemical detonation inside an ultracentrifuge,After,Centrifuge Explosion Damaged centrifuge well,Aluminum Dust + Freon = Kablooey,All Done!,Make sure you fill out the quiz, sign it, and drop it off in my mailbox for record keeping.Remember: This is an annual requirement. SEE YOU NEXT YEAR,Questions,Contact: Kathie Moh 255-8849kcm25cornell.edu,