EN 14123-2007 en Foodstuffs - Determination of aflatoxin B1 and the sum of aflatoxin B1 B2 G1 and G2 in hazelnuts peanuts pistachios figs and paprika powder - High performance liqu.pdf
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1、BRITISH STANDARDBS EN 14123:2007Foodstuffs Determination of aflatoxin B1and the sum of aflatoxin B1, B2, G1and G2in hazelnuts, peanuts, pistachios, figs, and paprika powder High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column cleanupThe European St
2、andard EN 14123:2007 has the status of a British StandardICS 67.050g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58BS EN 14123:2007This British Stan
3、dard was published under the authority of the Standards Policy and Strategy Committee on 31 January 2008 BSI 2008ISBN 978 0 580 58514 2National forewordThis British Standard is the UK implementation of EN 14123:2007. It supersedes BS EN 14123:2003 which is withdrawn.The UK participation in its prepa
4、ration was entrusted to Technical Committee AW/-/3, Food analysis Horizontal methods.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for it
5、s correct application.Compliance with a British Standard cannot confer immunity from legal obligations.Amendments/corrigenda issued since publicationDate CommentsEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 14123 December 2007 ICS 67.050 Supersedes EN 14123:2003 English Version Foodstuffs -
6、Determination of aflatoxin B1and the sum of aflatoxin B1, B2, G1and G2in hazelnuts, peanuts, pistachios, figs, and paprika powder - High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column cleanup Produits alimentaires - Dosage de laflatoxine B1et de l
7、a somme des aflatoxines B1, B2, G1et G2dans les noisettes, les cacahutes, les pistaches, les figues et le paprika en poudre - Mthode par purification sur colonne dimmuno-affinit suivie dune chromatographie liquide haute performance avec drivation post-colonne Lebensmittel - Bestimmung von Aflatoxin
8、B1und der Summe von Aflatoxin B1, B2, G1und G2in Haselnssen, Erdnssen, Pistazien, Feigen und Paprikapulver - Hochleistungsflssigchromatographisches Verfahren mit Immunaffinittssulen-Reinigung und Nachsulenderivatisierung This European Standard was approved by CEN on 12 November 2007. CEN members are
9、 bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to t
10、he CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same
11、 status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Ro
12、mania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means reser
13、ved worldwide for CEN national Members. Ref. No. EN 14123:2007: EEN 14123:2007 (E) 2 Contents Page Foreword3 1 Scope 3 2 Normative references 3 3 Principle3 4 Reagents.4 5 Apparatus .7 6 Procedures .9 7 Precision.13 8 Test report 16 Annex A (informative) Typical chromatograms .18 Annex B (informativ
14、e) Precision data24 Bibliography 29 EN 14123:2007 (E) 3 Foreword This document (EN 14123:2007) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, e
15、ither by publication of an identical text or by endorsement, at the latest by June 2008, and conflicting national standards shall be withdrawn at the latest by June 2008. This document supersedes EN 14123:2003 with the following changes: a) Validation data on hazelnut are included. WARNING The use o
16、f this standard can involve hazardous materials, operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicabi
17、lity of regulatory limitations prior to use. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, German
18、y, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. 1 Scope This European Standard is applicable to the determination of aflatoxins B1, B2, G1and G2in
19、 hazelnuts, figs, pistachios, peanuts and paprika powder. The limit of quantification of the method is 0,8 ng/g for each aflatoxin or better (value derived from in-house and collaborative study), depending on the equipment used. 2 Normative references The following referenced documents are indispens
20、able for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
21、 3 Principle A test portion is either extracted with a solvent solution (methanol/water) or the solvent solution plus hexane (or cyclohexane). The sample extract is filtered, diluted with phosphate buffered saline (PBS) and applied to an immunoaffinity column (IAC) containing antibodies specific to
22、aflatoxins B1, B2, G1and G2. The aflatoxins are eluted from the immunoaffinity column with methanol. Aflatoxins are quantified by reverse-phase high performance liquid chromatography (RP-HPLC) with post-column derivatization (PCD) involving bromination followed by fluorescence detection. The PCD is
23、achieved with either electrochemically generated bromine or with pyridinium hydrobromide perbromide (PBPB). EN 14123:2007 (E) 4 4 Reagents 4.1 General Use only reagents of recognized analytical grade and water complying with grade 3 of EN ISO 3696, unless otherwise specified. 4.2 Water, complying wi
24、th grade 1 of EN ISO 3696 4.3 Phosphate buffered saline (PBS), pH = 7,4 Dissolve 0,20 g of potassium chloride, 0,20 g of potassium dihydrogen phosphate, 1,16 g of disodium hydrogen orthophosphate (or 2,92 g of hydrogenphosphate12 H20) and 8,00 g of sodium chloride in 0,9 l of water. After dissolutio
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