CEN TR 16193-2013 Sludge treated biowaste and soil - Detection and enumeration of Escherichia coli《污泥 经处理的生物废物和土壤 大肠杆菌的探测和计数》.pdf
《CEN TR 16193-2013 Sludge treated biowaste and soil - Detection and enumeration of Escherichia coli《污泥 经处理的生物废物和土壤 大肠杆菌的探测和计数》.pdf》由会员分享,可在线阅读,更多相关《CEN TR 16193-2013 Sludge treated biowaste and soil - Detection and enumeration of Escherichia coli《污泥 经处理的生物废物和土壤 大肠杆菌的探测和计数》.pdf(56页珍藏版)》请在麦多课文档分享上搜索。
1、BSI Standards PublicationSludge, treated biowaste and soil Detection and enumeration of Escherichia coliPD CEN/TR 16193:2013National forewordThis Published Document is the UK implementation of CEN/TR 16193:2013.This technical report contains details of three methods evaluated for theirsuitability fo
2、r horizontal application for sludge, treated biowaste and soil.The UK committee believes that these methods are not fit for their intendedpurpose and are not appropriate to samples of all such materials, as the sample matrices are not sufficiently similar.It should be noted that in merging three sep
3、arate drafts to achieve the finaltext, incompatibilities and conflicting interpretation have been introduced: the document does not adhere to a recognised definition of E. coli as target organism (see SCA methods referred to below); the inter laboratory validation undertaken (as found in Tables 4, 9
4、 and 14)is considered by UK technical experts as demonstrating poor reproducibility; soil matrices were not included in the validation.The recommended methods for UK laboratories intending to analyze waste-water sludge can be found in The Microbiology of Sewage Sludge: Part 3(2003) Methods for the i
5、solation and enumeration of Escherichia coli,including verocytotoxigenic Escherichia coli published by the EnvironmentAgency Standing Committee of Analysts (SCA) in the series Methods for theexamination of water and associated materials. SCA methods are also considered suitable for determining E. co
6、li in sometreated biowaste applications such as compost, under PAS 100:2005, anddigestates, under PAS 110:2010. BS ISO 16649-2:2001 provides a methodsuitable for enumeration of E. coli in food and animal feeding stuffs. Thesemethods might also be applicable to soil but this would need to be verified
7、using the approach described in DD ENV ISO/TR 13843:2001, Water Quality Guidance on Validation of Microbiological Methods.The UK participation in its preparation was entrusted to Technical CommitteeH/-/4, Environmental testing programmes.A list of organizations represented on this committee can be o
8、btained onrequest to its secretary.This publication does not purport to include all the necessary provisions of acontract. Users are responsible for its correct application. The British Standards Institution 2013Published by BSI Standards Limited 2013ISBN 978 0 580 70860 2ICS 13.030.01Compliance wit
9、h a British Standard cannot confer immunity fromlegal obligations.This Published Document was published under the authority of the Standards Policy and Strategy Committee on 31 July 2013.Amendments issued since publicationAmd. No. Date Text affectedPUBLISHED DOCUMENTPD CEN/TR 16193:2013TECHNICAL REP
10、ORT RAPPORT TECHNIQUE TECHNISCHER BERICHT CEN/TR 16193 May 2013 ICS 13.030.01 English Version Sludge, treated biowaste and soil - Detection and enumeration of Escherichia coli Boue, biodchet trait et sol - Recherche et dnombrement des Escherichia coli Schlamm, behandelter Bioabfall und Boden - Nachw
11、eis und Zhlung von Escherichia coli This Technical Report was approved by CEN on 1 March 2011. It has been drawn up by the Technical Committee CEN/TC 400. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Y
12、ugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EURO
13、PEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. CEN/TR 16193:2013: EPD CEN/TR 16193:2013CEN/TR 16193:2013 (E) 2 Contents Pag
14、e Foreword . 4 Introduction 5 1 Scope 6 2 Normative references . 6 3 Terms and definitions 6 4 Abbreviations . 7 5 Quality assurance 7 6 Method A Membrane filtration method for quantification 7 6.1 Scope 7 6.2 Principle 8 6.3 Reagents, diluents and culture media . 8 6.4 Apparatus . 9 6.5 Sampling .
15、10 6.5.1 General . 10 6.5.2 Storage . 11 6.5.3 Handling . 11 6.6 Procedure . 11 6.6.1 Sample preparation 11 6.6.2 Sample dilution 12 6.6.3 Membrane filtration 12 6.6.4 Resuscitation and enumeration of colonies on chromogenic agar . 13 6.6.5 Confirmation of colony identity . 13 6.6.6 Determination of
16、 the dry residue content . 13 6.7 Calculation and expression of results 13 6.8 Performance data of the interlaboratory comparison Method A . 14 6.8.1 Material used in the interlaboratory comparison study . 14 6.8.2 First assessment of the precision of the method . 15 6.8.3 Interlaboratory comparison
17、 results . 16 6.9 Pre-filtration and centrifugation Comparison tests 17 7 Method B Miniaturised method (Most Probable Number) by inoculation in liquid medium . 19 7.1 Scope 19 7.2 Principle 19 7.3 Reagents, diluents and culture media . 19 7.4 Apparatus . 21 7.5 Sampling . 22 7.5.1 General . 22 7.5.2
18、 Storage . 22 7.5.3 Handling . 22 7.6 Procedure . 22 7.6.1 Sample preparation 22 7.6.2 Analysis 23 7.6.3 Determination of the dry residue content . 24 7.7 Expression of results . 24 7.7.1 Determination of the characteristic number . 24 7.7.2 Calculation of the MPN and its confidence interval 25 7.8
19、Performance data . 27 7.8.1 MPN Statistical table 27 PD CEN/TR 16193:2013CEN/TR 16193:2013 (E) 3 7.8.2 Performance data of the interlaboratory comparison . 35 7.8.3 First assessment of the precision of the method . 36 7.8.4 Interlaboratory comparison results . 37 7.9 Preparation of synthetic sea sal
20、t . 39 7.9.1 Major ion composition of a convenient ocean synthetic sea salt 39 7.9.2 Example for preparation from defined substances 39 7.10 Quality criteria for the manufacturing of the medium in microtitre plates (E. coli) . 40 8 Method C Macromethod (Most Probable Number) in liquid medium . 41 8.
21、1 Scope 41 8.2 Principle 41 8.3 Reagents, diluents and culture media . 41 8.4 Apparatus . 42 8.5 Sampling . 43 8.5.1 General . 43 8.5.2 Sample storage. 43 8.5.3 Sample handling . 43 8.6 Procedure . 43 8.6.1 Sample preparation 43 8.6.2 Analysis 44 8.6.3 Determination of the dry residue content . 44 8
22、.7 Expression of the results . 44 8.8 Performance data . 46 8.8.1 MPN Statistical table for 3-tubes MPN procedure . 46 8.8.2 Repeatability and reproducibility . 47 8.8.3 First assessment of the precision of the method . 47 8.8.4 Interlaboratory comparison results . 48 9 Test report 50 Bibliography 5
23、1 PD CEN/TR 16193:2013CEN/TR 16193:2013 (E) 4 Foreword This document (CEN/TR 16193:2013) has been prepared by Technical Committee CEN/TC 400 “Project Committee - Horizontal standards in in the fields of sludge, biowaste and soil”, the secretariat of which is held by DIN. Attention is drawn to the po
24、ssibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document has been prepared under a mandate given to CEN by the European Commission and the European Free Trad
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