GOST 31480-2012 Mixed fodders raw mixed fodders Determination of aminoacids (lysine methionine threonine cystine and triptophane) content by method of capillary electrophoresis《混合饲.pdf

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1、, (, , ) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)314802012, - 1.092 . 1.22009 . , . , , , - 1 - ( ), - - ( ), ( )2 3 , (- 2324 2012 . 41) : ( 3166) 00497 ( 3166) 00497 AZAMKGRU4 28 - 2012 . 465- 314802012 - 12013 .5 5234720056 ( ) - . , . , 2012 -, II 3148020121 1

2、2 13 24 25 .26 , , 37 98 .12 () , 13 () .14 15III 314802012, (, , , ) Mixed fodders, raw mixed fodders. Determination of aminoacids (lysine, methionine,threonine, cystine and triptophane) content by method of capillary electrophoresis 201307011 : , , , . () -. - .2 : 12.1.00491 . . - 12.1.00776 . .

3、12.1.01893 . - . 12.1.01979 . . - 177074 . , , , . 311877 . . 410778 . 8-. 419976 . 10-. 420477 . . 432877 . 670972 . 914780 . 1052178 . . 13496.080 , . 13586.383 . 13979.086 , . 1491983 , . - 1631787 . 1708387 . 1768182 . 1 314802012 1830087 . 2140075 -. . 241042001 . 2533682 . , 2726287 . 2766888

4、. 2916991 ( 64877) . 2922791 ( 835-181) . . 1. - , 1 , , . (), (-) . , , , , .3 () , - 1 ( = 0,95). 1 ( )c115r,% ( ) c115R,% ( ) c177c100,%, /3: 0,25 1,0 . 1,0 2,5 2,5 10 , /3: 0,3 1,0 . 1,0 3,0 , /3: 0,25 1,0 . 1,0 3,0 , /3: 0,2 2,0 ., /3: 0,1 2,0 .20151020152015201125201425202518251550402850405036

5、50304 4.1 - 12.1.007, 12.1.004, 12.1.018 12.1.019.4.2 , , - -. .4.3 - .5 5.1 13496.0, 13586.3, 13979.0, 17681, 27262, 27668.5.2 1 - , .2 3148020126 , , 6.1 , , 1 25 , , , 190200 , - ( 65 , 0,05 ), -105.- Windows, 1.5. 24104 - c177 0,001 c177 0,02 . 16317 .- ( c177 0,05 . ). 2.779.040 6000 /. , - (11

6、0 c177 2) . 14919. 17083. 25336. . 10, 20, 100, 500 1000 3. -1-25(1000)-14/23(19/26, 24/29) 25336. 1(2)-25(50, 100, 1000)-2 1770. 1(2, 3, 5)-1(1, 2, 2)-2-1(5, 10) 29227. 1 (2)-2-1(5, 10, 20) 29169. 550, 50200, 2001000 3 c177 5 % , . 2-25 25336. ( ) 1,5 3 , . () -14/8 (19/9, 24/10, 34/12) 25336. -25(

7、36)-36(80) 25336. 20 3 21400. 15 3 . - ( 0,2 , 25 ). , -. 4328, (NaOH) = 0,5 /3.L- , L-, L-, L-, L- ., ( D-, DL-). 3118, (Cl) = 1 /3, (HCI) = 0,1 /3, (HCI)=6/3.c98-, , . , -. 10- 4199, (Na2B4O7c215 10H2O) = 0,05 /3 (Na2B4O7c21510H2O) = 0,02 /3. 10521. 6709.1 , .2 . . . . .3 , , .3 3148020126.2 6.2.1

8、 6.2.1.1 (HCl)=1/38,3 3 100 3 . - . .6.2.1.2 (NaOH) = 0,5 /3 2,000 100 3, - 5060 3 - . 2 . 6.2.1.1 6.2.1.2 .6.2.1.3 (Na2B47c21510H2) = 0,05 /3 - , . 2 . - 10- -. 19,071 1 3 - . 20 9,22 c177 0,10.6.2.1.4 (HCl)=6/3100 3 . . .6.2.1.5 (HCl) = 0,1 /3 - . .6.2.2 6.2.2.1 (Na2B47c21510H2) = 0,02 /320 3 , 6.2.1.3, 50 3 . :- ;- ;- ( ) , - ;- .6.2.2.2 c98-