BS EN ISO 11816-2-2016 Milk and milk products Determination of alkaline phosphatase activity Fluorimetric method for cheese《牛奶和奶制品 碱性磷酸酶的活性测定 乳酪的荧光计法》.pdf
《BS EN ISO 11816-2-2016 Milk and milk products Determination of alkaline phosphatase activity Fluorimetric method for cheese《牛奶和奶制品 碱性磷酸酶的活性测定 乳酪的荧光计法》.pdf》由会员分享,可在线阅读,更多相关《BS EN ISO 11816-2-2016 Milk and milk products Determination of alkaline phosphatase activity Fluorimetric method for cheese《牛奶和奶制品 碱性磷酸酶的活性测定 乳酪的荧光计法》.pdf(26页珍藏版)》请在麦多课文档分享上搜索。
1、BS EN ISO 11816-2:2016Milk and milk products Determination of alkalinephosphatase activityPart 2: Fluorimetric method for cheese(ISO 11816-2:2016)BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS EN ISO 11816-2:2016 BRITISH STANDARDNational forewordThis British Stan
2、dard is the UK implementation of EN ISO11816-2:2016. It supersedes BS EN ISO 11816-2:2003 which iswithdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee AW/5, Chemical analysis of milk and milk products.A list of organizations represented on this committee can beobtain
3、ed on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2016.Published by BSI Standards Limited 2016ISBN 978 0 580 76892 7ICS 67.100.30Compliance with a
4、British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 30 September 2016.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dEUROPEAN STANDARD NORME EUROPENNE EUROPIS
5、CHE NORM EN ISO 11816-2 August 2016 ICS 67.100.30 Supersedes EN ISO 11816-2:2003English Version Milk and milk products - Determination of alkaline phosphatase activity - Part 2: Fluorimetric method for cheese (ISO 11816-2:2016) Lait et produits laitiers - Dtermination de lactivit de la phosphatase a
6、lcaline - Partie 2: Mthode fluorimtrique pour le fromage (ISO 11816-2:2016) Milch und Milchprodukte - Bestimmung der Aktivitt der alkalischen Phosphatase - Teil 2: Fluorimetrisches Verfahren fr Kse (ISO 11816-2:2016) This European Standard was approved by CEN on 29 July 2016. CEN members are bound t
7、o comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-C
8、ENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has
9、the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxe
10、mbourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brusse
11、ls 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11816-2:2016 EBS EN ISO 11816-2:2016EN ISO 11816-2:2016 (E) 3 European foreword This document (EN ISO 11816-2:2016) has been prepared by Technical Committee ISO/TC 34 “Foo
12、d products” in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of sampling and analysis” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement,
13、 at the latest by February 2017, and conflicting national standards shall be withdrawn at the latest by February 2017. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifyin
14、g any or all such patent rights. This document supersedes EN ISO 11816-2:2003. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic
15、, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsem
16、ent notice The text of ISO 11816-2:2016 has been approved by CEN as EN ISO 11816-2:2016 without any modification. BS EN ISO 11816-2:2016ISO 11816-2:2016(E)IDF 155-2:2016(E)Forewords .iv1 Scope . 12 Normative reference . 13 Terms and definitions . 14 Principle 15 Reagents 26 Apparatus . 37 Sampling 4
17、8 Preparation of test sample . 49 Procedure. 49.1 Verification of instrument performance . 49.1.1 General 49.1.2 Daily instrument tests . 59.1.3 Controls 59.2 Reagent controls to test the suitability of ready to use working substrate (5.3) 59.3 Calibration 69.4 Determination . 69.5 Test sample relat
18、ed controls . 79.5.1 Recommended negative and positive control tests .79.5.2 Interfering substance test 89.5.3 Heat-stable microbial alkaline phosphatase control test . 810 Calculation and expression of results . 810.1 Calibration ratio . 810.2 Calculation 910.2.1 Supernatant 910.2.2 Cheese . 910.3
19、Expression of results . 1011 Precision 1011.1 Interlaboratory test 1011.2 Repeatability 1011.3 Reproducibility 1012 Test report 10Annex A (informative) Interlaboratory trial .11Annex B (informative) Examples of preparation of a test sample 13Bibliography .15 ISO and IDF 2016 All rights reserved iiiC
20、ontents PageBS EN ISO 11816-2:2016ISO 11816-2:2016(E)ForewordsISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. E
21、ach member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Internati
22、onal Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the differen
23、t types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall no
24、t be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is inf
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