ASTM D3598-1989(2009) Standard Test Method for Citrate in Synthetic Detergents《合成清洁剂中的柠檬酸盐的标准试验方法》.pdf
《ASTM D3598-1989(2009) Standard Test Method for Citrate in Synthetic Detergents《合成清洁剂中的柠檬酸盐的标准试验方法》.pdf》由会员分享,可在线阅读,更多相关《ASTM D3598-1989(2009) Standard Test Method for Citrate in Synthetic Detergents《合成清洁剂中的柠檬酸盐的标准试验方法》.pdf(3页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: D3598 89 (Reapproved 2009)Standard Test Method forCitrate in Synthetic Detergents1This standard is issued under the fixed designation D3598; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revision. A
2、number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers the enzymatic determination ofcitrate in both liquid and solid synthetic detergents. The testmethod is applicab
3、le to most detergents containing citrate at aminimum concentration of approximately 5 % (1-8).21.2 The values stated in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.1.3 This standard does not purport to address all of thesafety concerns, if any,
4、associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use. Material SafetyData Sheets are available for reagents and materials. Reviewthem for hazards pri
5、or to usage.2. Referenced Documents2.1 ASTM Standards:3D501 Test Methods of Sampling and Chemical Analysis ofAlkaline DetergentsD1193 Specification for Reagent Water3. Summary of Test Method3.1 This test method employs an enzyme system that isbased upon the selective cleavage of citrate by citrate l
6、yase(citrate oxaloacetate-lyase; EC 4.1.3.6) (1). One of the prod-ucts, oxaloacetate, is reduced to malate by malic dehydroge-nase (L-malate: NAD oxidoreductase; EC 1.1.1.37) with thesimultaneous oxidation of reduced b-nicotinamide adeninedinucleotide to b-nicotinamide adenine dinucleotide, oxidized
7、form. The course of the reaction is measured spectrophoto-metrically. The decrease in absorbance at 340 nm caused by theformation of b-nicotinamide adenine dinucleotide, oxidizedform, is directly proportional to the concentration of citrate.4. Interferences4.1 The test method is highly specific for
8、citrate. Otherorganic acids, for example, cisand trans-aconitic, d,l-isocitric,a-ketoglutaric, oxalic, succinic, or tartaric acids, do not inter-fere.4.2 Although low levels of zinc or magnesium, or both, arerequired as an activator for the enzyme citrate lyase, exces-sively high levels of divalent
9、metallic ions including zinc andmagnesium will cause inactivation of the enzyme and poten-tially interfere with the test method (7).4.3 The test method is not applicable to those detergentscontaining components with excessive absorptivity at 340 nmsuch that ultraviolet measurements are inappropriate
10、 at 340 nmunder test conditions.5. Apparatus5.1 Interval Timer.5.2 Micropipet, suitable Eppendorf pipets for dispensing 10and 100-L volumes and with disposable tips.5.3 Spectrophotometer, suitable for measuring ultravioletabsorbance at 340 nm and equipped with 1-cm matched quartzcells with tapered T
11、FE-fluorocarbon stoppers and a minimumvolume of 4 mL.6. Reagents6.1 Purity of ReagentsReagent grade chemicals shall beused in all tests. Unless otherwise indicated, it is intended thatall reagents shall conform to the specifications of the Commit-tee on Analytical Reagents of the American Chemical S
12、ociety,where such specifications are available.4Other grades may beused, provided it is first ascertained that the reagent is of1This test method is under the jurisdiction of ASTM Committee D12 on Soapsand Other Detergents and is the direct responsibility of Subcommittee D12.12 onAnalysis and Specif
13、ications of Soaps, Synthetics, Detergents and their Components.Current edition approved Oct. 1, 2009. Published December 2009. Originallyapproved in 1977 as D3598 77 T. Last previous edition approved in 2003 asD3598 89 (2003). DOI: 10.1520/D3598-89R09.2The boldface numbers in parentheses refer to th
14、e references at the end of thistest method.3For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.4Reagent Chemicals
15、, American Chemical Society Specifications , AmericanChemical Society, Washington, DC. For suggestions on the testing of reagents notlisted by the American Chemical Society, see Analar Standards for LaboratoryChemicals, BDH Ltd., Poole, Dorset, U.K., and the United States Pharmacopeiaand National Fo
16、rmulary, U.S. Pharmacopeial Convention, Inc. (USPC), Rockville,MD.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.sufficiently high purity to permit its use without lessening theaccuracy of the determination.6.2 Purity of WaterUnless
17、 otherwise indicated, referencesto water shall be understood to mean Type II reagent waterconforming to Specification D1193.6.3 Citrate Lyase Solution (40 units/mL)Add sufficientcold water to a vial of citrate lyase containing a premeasuredweight of enzyme protein such that the resulting solution wi
18、llcontain 40 units/mL; for example, 2.0 mL of water is added toa vial containing 5 mg of enzyme protein with an activity of 16units/mg of enzyme protein. One unit of activity will convert1.0 mol of citrate to oxaloacetate per minute at pH 7.6 at25C. The citrate lyase solution should be maintained in
19、 an icebath for the duration of the analyses and can be used for 5 daysif refrigerated. Citrate lyase (EC 4.1.3.6) from Aerobacteraerogenes is commercially available as a lyophilized powdercontaining approximately 24 % citrate lyase, 24 % albumin,48 % saccharose and 4 % magnesium sulfate (MgSO47H2O)
20、.The citrate lyase powder should be stored as specified by thesupplier.6.4 Disodium b-Nicotinamide Adenine Dinucleotide, Re-duced Form Solution (0.0032 M)Dissolve 10 mg of disodi-umb -nicotinamide adenine dinucleotide, reduced form, (b-NADH) in 4.0 mL of water. The b-NADH should beapproximately 98 %
21、 pure and essentially free of the alphaisomer. The b-NADH solution should be protected from lightand maintained in an ice bath for the duration of the analyses.The solution should be prepared fresh daily.6.5 Hydrochloric Acid (sp gr 1.19)Concentrated hydro-chloric acid (HCl).6.6 Hydrochloric Acid (1
22、 N)Slowly add 85 mL of HCl (spgr 1.19) to 700 mL of water and with mixing dilute to 1 L withwater.6.7 Malic Dehydrogenase Solution (2500 units/mL) Addsufficient cold water to a vial of malic dehydrogenase (MDH)suspension containing a premeasured volume such that theresulting solution will contain 25
- 1.请仔细阅读文档,确保文档完整性,对于不预览、不比对内容而直接下载带来的问题本站不予受理。
- 2.下载的文档,不会出现我们的网址水印。
- 3、该文档所得收入(下载+内容+预览)归上传者、原创作者;如果您是本文档原作者,请点此认领!既往收益都归您。
下载文档到电脑,查找使用更方便
10000 积分 0人已下载
下载 | 加入VIP,交流精品资源 |
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- ASTMD359819892009STANDARDTESTMETHODFORCITRATEINSYNTHETICDETERGENTS 合成 清洁剂 中的 柠檬酸 标准 试验 方法 PDF

链接地址:http://www.mydoc123.com/p-515075.html