DB45 T 2029-2019 甘蔗白条病菌PCR检测技术规程.pdf

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1、g44g38g54g3 g25g24g17g19g21g19g17g19g20g3 g37g3g20g25g3 g3g3g3g3g3g3 DB45 g5301g16309g3876g7173g14368g8945g2416g3430g7151g7741g2044g3 g39g37g3g23g24g18g55g3g21g19g21g28g258g21g19g20g28g3 g3g3g3g3g3g3 g11086g15181g11443g7575g11259g14850g51g38g53g7926g9089g6326g7525g16378g12353g3 g39g72g87g72g70g87g76

2、g81g74g3g87g72g70g75g81g76g84g88g72g3g85g72g74g88g79g68g87g76g82g81g86g3g73g82g85g3g83g68g87g75g82g74g72g81g3g82g73g3g59g68g81g87g75g82g80g82g81g68g86g3g68g79g69g76g79g76g81g72g68g81g86g3 g11g36g86g75g69g92g12g39g82g90g86g82g81g3g69g68g86g72g71g3g82g81g3g51g38g53g3 g3 g3 g3 g21g19g20g28g3g16g3g20g21

3、g3g16g3g19g24g2567g5177g3 g21g19g20g28g3g16g3g20g21g3g16g3g22g19g4564g7155 g5301g16309g3876g7173g14368g8945g2416g5176g3440g11527g11673g12759g10812g4726g3 g3 g3 g2567g5177 g3 g39g37g23g24g18g55g3g21g19g21g28g178g21g19g20g28g3 g44g3 g2179g3 g3 g16438g3 g7516g7735g2038g6457g10135g42g37g18g55g3g20g17g20

4、g178g21g19g19g28g13577g2090g11444g16372g2121g17319g14713g452g3 g7516g7735g2038g11105g5295g16303g3870g7167g14362g8939g2410g13062g1098g2254g1948g4564g6656g2090g5286g11521g11667g4558g7149g452g3 g7516g7735g2038g11105g5295g16303g13062g1098g7735g2038g2374g6320g7519g4100g2696g1354g5506g2579g452g3 g7516g773

5、5g2038g17319g14713g2437g1405g726g5295g16303g3870g7167g14362g8939g2410g1996g1098g12289g4502g19602g11080g15175g11844g12454g6256g451g5295g16303g11080g15175g18055g1360g7017g14495g18429g9961g4558g20668g4564g452g3 g7516g7735g2038g1131g16305g17319g14713g1258g2696g726g19990g18433g14842g451g2120g7236g17849g4

6、51g16307g6495g5482g451g5456g4671g12283g451g5456g14739g2430g451g7794g5951g1217g451g20863g7253g10245g451g4539g1566g21631g451g7550 g7576g10894g451g2712g10746g451g21748g1100g1246g451g20172g7861g7136g451g2120g10944g452 g39g37g23g24g18g55g3g21g19g21g28g178g21g19g20g28g3 g20g3 g11086g15181g11443g7575g11259

7、g14850g51g38g53g7926g9089g6326g7525g16378g12353g3 g20g3 g14649g3370g3 g7516g7735g2038g16372g4554g1206g11080g15175g11437g7569g11253g14844g708g59g68g81g87g75g82g80g82g81g68g86g3g68g79g69g76g79g76g81g72g68g81g86g3g11g36g86g75g69g92g12g3g39g82g90g86g82g81g709g11444g51g38g53g7920g9083g11444g2511g10806g45

8、1g16901 g2162g451g1131g16305g1306g3224g1086g16878g3895g451g7783g2801g11444g18423g19702g451g1549g4488g2748g17920g17859g451g7920g9083g7145g8965g2748g13571g7628g6655g17952g2554g2132g4554g452g3 g7516g7735g2038g17970g11096g1214g11080g15175g11437g7569g11253g14844g62g59g68g81g87g75g82g80g82g81g68g86g3g68g7

9、9g69g76g79g76g81g72g68g81g86g3g11g36g86g75g69g92g12g3g39g82g90g86g82g81g64g11444g5659g17999g7920g9083g452g3 g21g3 g16378g14649g5725g5451g11102g7101g1324g3 g1083g2119g7095g1318g4649g1214g7516g7095g1318g11444g5316g11096g7263g5621g1085g2591g4673g11444g452g2065g7263g8984g7189g7503g11444g5445g11096g7095g

10、1318g712g1269g6256g8984g7189g7503g11444g10360g7516g17970g11096g1214g7516g7095 g1318g452g2065g7263g1085g8984g7189g7503g11444g5445g11096g7095g1318g712g1958g7472g7136g10360g7516g708g2357g6428g6256g7481g11444g1566g7017g2437g709g17970g11096g1214g7516g7095g1318g452g3 g42g37g18g55g3g25g25g27g21g178g21g19g1

11、9g27g3g3g2102g7616g4558g20668g4564g11096g8804g16372g7788g2748g16901g20668g7145g8965g3 g22g3 g2517g10812g3 g2137g11096g11080g15175g11437g7569g11253g11253g2511g11437g7569g21748g2437g14094g14844g20g25g16g21g22g85g53g49g36g3g11444g44g55g54g3626g3344g16878g16849g11444g10409g5426g5719g5482g11444g5445g1039

12、3g59g68g36g79g69g21g16g73g22g2748 g59g68g36g79g69g21g16g85g22g712g1301g11253g2511g14844g11444g39g49g36g1130g8273g7599g712g51g38g53g6297g3790g2616g6208g11550g11444g10359g8677g712g1278g13884g5659g17999g451g9893g7039g451g20744g7032g3424g4649g11080g15175g11437g7569g11253g14844 g708g59g68g81g87g75g82g80g

13、82g81g68g86g3g68g79g69g76g79g76g81g72g68g81g86g3g11g36g86g75g69g92g12g3g39g82g90g86g82g81g709g17931g15996g7920g9083g452g3 g23g3 g16907g2168g3 g23g17g20g3 g6256g11096g16901g2162g3447g1130g2102g7616g13535g16901g2162g712g4558g20668g4564g11096g8804g12630g2616g42g37g18g55g3 g3 g25g25g27g21g252g21g19g19g2

14、7g1117g1212g13527g8804g6455g7735g712g1958g1117g9145g2554g51g38g53g11444g11096 g8804g5316g12630g2616g1072g13527g8804g6455g7735g452g3 g23g17g21g3 g9186g8798g452g3 g23g17g22g3 g7997g10393g3626g3344g13556g39g49g36g6656g2566g16901g2162g11522g452g3 g23g17g23g3 g42g82g79g71g72g81g3g89g76g72g90g7784g18344g7

15、683g7113g452g3 g23g17g24g3 g21g238g40g68g86g92g55g68g84g3g51g38g53g3g2557g5316g20148g9255g9186g726g5170g2910g1239g2801g452g9091g5334g1130g20g19 g3 g80g80g82g79g18g47g712g1958g1117g2651g7481g19g17g19g24 g3 g56g18g541g47g3g55g68g84g3g39g49g36g3 g51g82g79g92g80g72g85g68g86g72g451g19g17g23 g3 g48g80g3g7

16、1g49g55g51g3g48g76g91g87g88g85g72g451g23 g3 g80g48g48g74 g21g14 g451g21g238g51g38g53g3g37g88g73g73g72g85g452g3 g23g17g25g3 g20g238g55g40g13635g2018g9186g452g3 g23g17g26g3 g20 g3 g24g19g19 g3 g69g83g3g39g49g36g2102g4480g17240g18431g7735g2038g10393g452g3 g23g17g27g3 g10860g14130g13062g712g11109g8995g1

17、3527g452g3 g23g17g28g3 g7920g9083g5445g10393g5311g2119g726g3 g252g252g1082g9320g5445g10393g59g68g36g79g69g21g16g73g22g726g24g182g16g3g38g36g38g36g38g36g38g36g38g36g36g55g36g38g36g42g38g36g55g55g42g38g42g42g3g16g22g182g727g3 g252g252g1083g9320g5445g10393g59g68g36g79g69g21g16g85g22g726g24g182g16g3g38g

18、38g38g36g36g38g55g55g36g38g55g55g42g36g42g42g38g55g36g55g42g42g3g16g22g182g727g3 g252g252g20148g6297g3790g10359g8677g3927g4671g1130g23g19g19 g3 g69g83g147g24 g3 g69g83g452g3 g23g17g20g19g3 g7735g7783g726g19555g5719g4649g10135g1130g5154g11934g4554g5967g7683g11080g15175g11437g7569g11253g14844g11444g77

19、83g2801g3626g3344g13556g39g49g36g727g19556g5719g4649g10135g1130g11934g4554g7514g5967g7683g11080g15175g11437g7569 g11253g14844g11444g7783g2801g3626g3344g13556g39g49g36g452g3 g3 g39g37g23g24g18g55g3g21g19g21g28g178g21g19g20g28g3 g21g3 g24g3 g1137g16311g1312g3230g1092g16884g3901g3 g24g17g20g3 g51g38g53

20、g6297g3790g1306g452g3 g24g17g21g3 g2061g14118g6208g1791g13099g13583g452g3 g24g17g22g3 g7784g18344g15611g11437g2102g7616g1306g6214g13147g3910g2102g1913g1913g5334g16849g452g3 g24g17g23g3 g2592g5439g20744g17999g2023g2027g12267g5619g7530g726g12267g5619g2251g20g21 g3 g19g19g19 g3 g74g1301g1082g452g3 g24g

21、17g24g3 g5762g9305g8804g9124g19253g452g3 g24g17g25g3 g2016g12769g726g21 g3 g263g794g23 g3 g263g451g16g21g19 g3 g263g451g16g27g19 g3 g263g452g3 g24g17g26g3 g20744g2491g9885g14844g19253g452g3 g24g17g27g3 g21827g20222g5282g10261g12769g452g3 g24g17g28g3 g11109g4480g3929g5283g726g5967g18431g19g17g19g19g2

22、0 g3 g74g452g3 g24g17g20g19g3 g5598g18431g2256g7783g3224g726g19g17g20 g3 g173g47g794g21g17g24 g3 g173g47g451g19g17g24g3g173g47g794g20g19 g3 g173g47g451g21 g3 g173g47g794g21g19 g3 g173g47g451g20g19g3g173g47g794g20g19g19 g3 g173g47g451g21g19 g3 g173g47g794 g21g19g19 g3 g173g47g451g24g19 g3 g173g47g794

23、g20 g3 g19g19g19 g3 g173g47g452g3 g25g3 g7789g2807g11450g18429g19708g573g1555g4494g2754g17926g17865g3 g25g17g20g3 g7789g2807g18429g19708g3 g2591g18423g19702g2598g10359g7783g2801g6214g15175g14654g7783g2801g452g2598g10359g2566g7472g20744g2591g16369g14037g2506g5206g2598g708g14g20g2598g709g1117g18200g18

24、200g1405g2598g10359g11444g20g17g19 g3 g74g794g21g17g19 g3 g74g712g7022 g13726g1214g7783g2801g16059g1117g3895g11096g727g15175g14654g2566g6208g10191g7503g11080g15175g11444g1117g1083g18200g14654g14514g712g2539g11486g6484g8817g712g2566g8817g9186g13726g1214g24 g3 g80g79g794g20g19 g3 g80g79g12267g5619g127

25、53g1117g3895g11096g452g3 g25g17g21g3 g7789g2807g1555g4494g2754g17926g17865g3 g7783g2801g7022g13726g1214g21 g3 g263g794g27 g3 g263g7569g1318g1083g1549g4488g712g7206g19492g304g23g27 g3 g75g727g19375g7503g1549g4488g712g19760g7022g13726g3416g16g27g19 g3 g263g7569g1318g1083g712g1085g2591g2557g3901g2027g1

26、5805g452 g19375g17988g17920g17859g7206g712g2591g18423g11096g1549g9305g12769g1117g2256g5282g2016g6214g9186g8798g4598g4657g2622g17920g17969g452g3 g26g3 g7926g9089g7151g8971g3 g26g17g20g3 g7789g2807g39g49g36g6662g2572g3 g26g17g20g17g20g3 g2566g2598g10359g7783g2801g11444g1117g19492g18200g2102g19g17g20 g

27、3 g74g712g11096g9186g8798g2023g2027g11844g12056g14371g12985g7515g10470g712g6457g6256g17977g6425g11444g7997g10393g3626g3344g13556g39g49g36g6656g2566g16901g2162 g11522g6616g14720g11444g6909g1420g7145g8965g6656g2566g7783g2801g39g49g36g452g3 g26g17g20g17g21g3 g2566g15175g8817g7783g2801g20g17g24 g3 g80g4

28、7 g7022g1941g21 g3 g80g47g12267g5619g12753g1117g712g20g22 g3 g19g19g19 g3 g85g18g80g76g81g12267g5619g22 g3 g80g76g81g712g5427g1082g9269g712g2625g8889g9200g2256g1941g20 g3 g19g19g19g173g47 g9885g14844g8804g712g20g22 g3 g19g19g19 g3 g85g18g80g76g81g12267g5619g22 g3 g80g76g81g712g18429g3901g6909g1420g2

29、1g8529g712g2625g8889g9200g2256g1941g21g19 g3 g173g47g794g21g19g19 g3 g173g47 g9885g14844g8804g452g3 g26g17g21g3 g7789g2807g39g49g36g17246g18437g7926g9089g3 g7783g2801g39g49g36g6656g2566g2622g712g11096g15611g11437g18g7784g18344g2102g7616g1306g18596g4554g7783g2801g39g49g36g17240g18431g712g5507g36g21g2

30、5g19g18g36g21g27g19g8708g1644g1130g20g17g27g794g21g17g19g7206g712g17970g2616g1214g51g38g53 g6297g3790g452g3 g26g17g22g3 g51g38g53g7926g9089g3 g6457g16024g20g11444g7145g8965g18301g2150g21g24 g3 g173g47g11444g51g38g53g6297g3790g2557g5316g1411g13099g712g2557g5316g1411g13099g1117g2612g16901g2162g11444g1

31、8431g2591g7785g6558g2557g5316g1411g13099g11444g5739g1411g1411g12319g17931 g15996g17970g5507g16947g7076g452g51g38g53g6297g3790g2546g7072g726g28g23 g3 g263g20148g2568g5719g23 g3 g80g76g81g727g28g23 g3 g263g2568g5719g22g19 g3 g86g712g24g24 g3 g263g17968g9883g22g19 g3 g86g712g25g24 g3 g263g5414g1384g20

32、g3 g80g76g81g712g17931g15996 g22g20g1114g5594g10719g727g25g24g3g263g5414g1384g22 g3 g80g76g81g452g12458g11437g4649g10135g1130g7184g14844g1072g13527g8804g452g3 g3 g39g37g23g24g18g55g3g21g19g21g28g178g21g19g20g28g3 g22g3 g3 g16030g20g3g51g38g53g6303g3796g2563g5322g1417g13105g2552g7078g3 g16901g2162g3

33、g1455g11096g18431g3 g21g24 g3 g173g47g2557g5316g1411g13099g13560g9091g5334g3 g21g104g40g68g86g92g3g55g68g84g3g51g38g53g3g2557g5316g20148g9255g9186g3 g20g21g17g24 g3 g173g47g3 g20g104g3 g59g68g36g79g69g21g16g73g22g708g20g19g173g80g82g79g18g47g709g3 g20g17g19 g3 g173g47g3 g19g17g27 g3 g173g80g82g79g18

34、g47g3 g59g68g36g79g69g21g16g85g22g708g20g19g173g80g82g79g18g47g709g3 g20g17g19 g3 g173g47g3 g19g17g27 g3 g173g80g82g79g18g3g47g3 g8273g7599g39g49g36g11g21g24 g3 g81g74g18g173g47g12g3 g20g17g19 g3 g173g47g3 g21 g3 g81g74g18g173g47g3 g1072g13527g8804g3 g28g17g24 g3 g173g47g3 g18g3 g5739g1411g12319g3 g

35、21g24 g3 g173g47g3 g18g3 g26g17g23g3 g6303g3796g1245g10399g11450g11115g9001g7926g9089g3 g26g17g23g17g20g3 g12320g2566g17970g18431g10860g14130g13062g2256g1941g20g104g55g36g40g13635g2018g9186g1117g712g2256g10013g14371g4540g1944g9446g16403g712g18301g2150g6208g20g17g24g705g11444g10860g14130g13062g9446g9

36、186g727g12349g17970g2023 g2468g2622g1602g7599g712g4564g9305g1083g2061g3370g6208g10860g14130g13062g2061g14118g452g3 g26g17g23g17g21g3 g2102g2139g2566g21 g3 g173g47g3g25g104g1082g7783g13635g2018g9186g2748g24 g3 g173g47g11444g51g38g53g6297g3790g1239g10393g2144g7136g11444g51g38g53g12753g712g6495g14737g9

37、255g2352g712g21 g3 g19g19g19g3g74g12267g5619g24 g3 g86g452g3 g26g17g23g17g22g3 g2102g2139g4662g24 g3 g173g47g3g39g49g36g2102g4480g18431g7735g2038g10393g2748g51g38g53g1239g10393g9255g2616g9186g1485g8529g2256g1941g10860g14130g13062g2061g14118g11444g9961g7783g4484g2622g17931g15996g11109g8995g712 g11109

38、g8995g1455g11096g11444g11109g2491g1130g27g19 g3 g57g712g11109g8995g7206g19492g1130g22g19 g3 g80g76g81g452g3 g26g17g24g3 g2067g14124g6214g1797g2108g7622g3 g4662g10860g14130g13062g2061g14118g13726g1214g2061g14118g6208g1791g13099g13583g6208g1791g1306g1082g712g7785g6558g39g49g36g2102g4480g18431g7735g168

39、64g2132g7133g6297g3790g2090g11444g11550g11444g7569g5206g3927g4671g712g4662g11109 g8995g13571g7628g11096g6397g10135g13099g13583g6397g10135g5522g6208g3374g10359g7095g1318g4488g7827g452g3 g27g3 g13577g7634g6661g17958g2560g2138g4560g3 g27g17g20g3 g17246g6621g7741g2044g3 g19555g5719g4649g10135g2090g10720

40、g1072g7569g3927g4671g1130g23g19g19 g3 g69g83g102g24 g3 g69g83g7569g5206g712g19556g5719g4649g10135g2748g12458g11437g4649g10135g7184g7569g5206g7206g2132g4554g1130g4558g20668g7481g7032g452g3 g27g17g21g3 g13577g7634g2138g4560g3 g27g17g21g17g20g3 g19555g5719g726g16091g7920g7783g2801g8995g18051g2090g10720

41、g1072g7569g3927g4671g1130g23g19g19 g3 g69g83g102g24 g3 g69g83g7569g5206g7206g712g2132g1130g19555g5719g712g5621g16305g7206g17931g15996g9083g5311g452g3 g27g17g21g17g21g3 g19556g5719g726g16091g7920g7783g2801g8913g7481g2090g10720g1072g7569g3927g4671g1130g23g19g19 g3 g69g83g102g24 g3 g69g83g7569g5206g712

42、g13567g18429g3901g21g8529g51g38g53g7920g9083g2622g1277g7514g2090g10720g6297g3790 g7569g5206g7206g712g2132g1130g19556g5719g452g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g726g1255g800g3486g1056g1430g1677g2296g5940g1696g2996g3221g1101g1 g4210g5628g4358g3149g4288g5527 g49g36g51g1 g3292g3645g2686g3126g5946g4653g1 g37g35g21g22g16g53g1g19g17g19g26g803g19g17g18g26g1 g2296g5940g1828g3007g5292g3572g1244g2249g1699g4386g4437g4781g4144g2124g4945g694g1278g1156g1 g10366g7545g1097g7487g3g3g1515g7545g5627g12460g3