KS J ISO 7251-2007 Microbiology of food and animal feeding stuffs-Horizontal method for the detection and enumeration of presumptive Escherichia coli -Most probable number techniqu.pdf
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1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS J ISO 7251 KS J ISO 7251 :2007 (2012 ) 2007 10 31 http:/www.kats.go.krKS J ISO 7251:2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) : :2002 10 31 :2007 10 31 :2012 12 28 : 20120814 : ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO 7251:
2、2007 (2012 ) Microbiology of food and animal feeding stuffsHorizontal method for the detection and enumeration of presumptive Escherichia coli Most probable number technique 2005 3 ISO 7251, Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of presump
3、tive Escherichia coliMost probable number technique , . ISO 4832 ISO/TC34 (SC9) . 1 37 44 (MPN) . . 44 . (10. ). 2 . . ( ) . KS H ISO 68871, , 10 1: 10 KS H ISO 68872, , 10 2: KS H ISO 68873, , 10 3: KS H ISO 68874, , 10 4: , , KS J ISO 7251:2007 KS H ISO 7218, ISO 8261, Milk and milk productsGenera
4、l guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination ISO/TS 111331:2000, Microbiology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 1:General guidelines on quality assurance for the
5、preparation of culture media in the laboratory ISO/TS 111332:2003, Microbiology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 2:Practical guidelines on performance testing of culture media 3 . 3.1 (presumptive Escherichia coli) 44 44 3.2 , mL g 4 4.1
6、4.1.1 . 4.1.2 37 48 . 24 48 . 4.1.3 , (EC ) 2 . 4.1.4 4.1.3 44 48 . 24 48 . 4.1.5 4.1.4 , (indol-free pepton wa ter) 2 . 4.1.6 4.1.5 44 48 . . 4.1.7 (4.1.1 ) 2 EC 44 . x g x mL “ ” “ ” . 4.2 2 KS J ISO 7251:2007 4.2.1 . 4.2.2 . , 10 . 4.2.3 37 48 . 24 48 . 4.2.4 , (EC ) 2 . 4.2.5 4.2.4 44 48 . 24 48
7、 . 4.2.6 4.2.5 , 2 . 4.2.7 4.2.6 44 48 . . 4.2.8 2 EC 44 MPN ( A ) . 5 , KS H ISO 7218 . 5.1 KS H ISO 68871 , ISO 8261 . 5.2 ( )(lauryl sulfate broth) 5.2.1 a) b) (enzymatic digest of plant and animal tissues) 40.0 g 20.0 g (lactose) 10.0 g 5.0 g (K 2 HPO 4 ) 5.5 g 2.75 g (KH 2 PO 4 ) 5.5 g 2.75 g (
8、sodium chloride) 10.0 g 5.0 g CH 3 (CH 2 ) 11 OSO 3 Na 0.2 g 0.1 g 1 000 mL 1 000 mL 5.2.2 3 KS J ISO 7251:2007 . 25 pH 6.80.2 pH . (6.6 ) 16 mm160 mm (6.4 ) 9 mL , (6.6 ) 18 mm180 mm 20 mm200 mm (6.4 ) 10 mL . 121 (6.1 ) 15 . . 5.3 EC ( ) 5.3.1 (enzymatic digest of casein) 20.0 g (lactose) 5.0 g 3(
9、bile salts No. 3) a1.5 g (K 2 HPO 4 ) 4.0 g (KH 2 PO 4 ) 1.5 g (sodium chloride) 5.0 g 1 000 mL a 1 5.3.2 . 25 pH 6.80.2 pH . (6.6 ) 16 mm160 mm (6.4 ) 10 mL . 121 (6.1 ) 15 . . 5.3.3 , ISO/TS 111331 ISO/TS 111332 . 5.4 (peptone water, indole free) 5.4.1 (enzymatic digest of casein) 10.0 g (sodium c
10、hloride) 5.0 g 1 000 mL 4 KS J ISO 7251:2007 5.4.2 . 25 pH 7.30.2 pH . 16 mm160 mm (6.4 ) 5 mL10 mL . 121 (6.1 ) 15 . 5.5 (Kovacs ) 5.5.1 4(4dimethylaminobenzaldehyde) 5.0 g 21 1(2methylbutan1ol or pentan1ol) 75.0 mL (hydrochloric acid)( 201.181.19 g/mL) 25.0 mL 5.5.2 5055 4 (4 dimethylaminobenzalde
11、hyde) . . 4 (KS H ISO 7218 ). . . 6 , . . 6.1 () ( ) KS H ISO 7218 6.2 (371) (441) . 6.3 (441) . 6.4 5 KS J ISO 7251:2007 16 mm160 mm 18 mm180 mm 20 mm200 mm . 6.5 / / 3 mm 10 L 6.6 (6.4 ) . 6.7 (total-delivery pipettes) 1 mL 10 mL . 6.8 pH 0.01 pH , 25 pH 0.1 . 7 , . . , . 8 . , . 9 9.1 9.1.1 KS H
12、ISO 6887 ISO 8261 . 1 mL 9 mL 5.2.1 b) ( 0.1 g 0.1 mL) , 10 mL 10 mL 5.2.1 a) ( 1 g 1 mL) . , x mL x g 9 x mL (KS H ISO 6887 ISO 8261 ) 90x mL 5.2.1 b) . , 5 mL 5 g 45 mL , 450 mL 5.2.1 b) . 5.2.1 a) . 9.1.2 ( ) 6 KS J ISO 7251:2007 (5.2 ) 37 (6.2 ) 242 . , (482) . (482) . ( , ) , . 48 EC 9.1.3 . 9.
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