ASTM D7855 D7855M-2013 4588 Standard Test Method for Determination of Mold Growth on Coated Building Products Designed for Interior Applications Using an Environmental Chamber and .pdf
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1、Designation: D7855/D7855M 13Standard Test Method forDetermination of Mold Growth on Coated Building ProductsDesigned for Interior Applications Using an EnvironmentalChamber and Indirect Inoculation1This standard is issued under the fixed designation D7855/D7855M; the number immediately following the
2、 designation indicates theyear of original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of lastreapproval. A superscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers a
3、n environmental chamber andthe conditions of operation to evaluate in a 4-week period therelative resistance to mold growth and microbial surfacedefacement on coated building products designed for interiorapplication using an indirect inoculation method. The appara-tus is designed so it can be easil
4、y built or obtained by anyinterested party.1.2 This test method can be used to evaluate the compara-tive resistance of coated building products to accelerated moldgrowth. Ratings do not imply a specific time period that thecoated building product will be free of fungal growth duringinstallation in a
5、n interior environment.1.3 This test method is not intended for use in the evaluationof public health claims.1.4 The test method is intended for the accelerated evalua-tion of mold growth on a coated building product designed forinterior use. This method is not intended for evaluation ofsurfaces des
6、igned for exterior applications or uncoated sur-faces. Use of this test method for evaluating exterior perfor-mance has not been validated, nor have the limitations for suchuse been determined.1.5 The values stated in either SI units or inch-pound unitsare to be regarded separately as standard. The
7、values stated ineach system may not be exact equivalents; therefore, eachsystem shall be used independently of the other. Combiningvalues from the two systems may result in non-conformancewith the standard.1.6 This standard does not purport to address all of thesafety concerns, if any, associated wi
8、th its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2D16 Terminology for Paint, Related Coatings, Materials, andApplica
9、tionsD1193 Specification for Reagent WaterD6329 Guide for Developing Methodology for Evaluatingthe Ability of Indoor Materials to Support MicrobialGrowth Using Static Environmental ChambersE177 Practice for Use of the Terms Precision and Bias inASTM Test MethodsE691 Practice for Conducting an Interl
10、aboratory Study toDetermine the Precision of a Test Method3. Terminology3.1 DefinitionsFor definitions of terms refer to Terminol-ogy D16.3.2 Definitions of Terms Specific to This Standard:3.2.1 chamber control, nopen Petri dish containing appro-priate agar to demonstrate viability of fungal organis
11、ms withinthe environmental chamber.3.2.2 coated building product, na building material hav-ing a liquid, liquefiable or mastic composition that is convertedto a solid protective, decorative, or functional adherent filmafter application as a thin layer onto a building fabric.3.2.3 interior, nany surf
12、ace not exposed to exterior envi-ronments in end use.3.2.4 interior finish, ninterior wall and ceiling finish andinterior floor finish.3.2.5 material control, nuntreated representative sub-strate.3.2.6 sample, na portion of material taken from a largerquantity for the purpose of estimating propertie
13、s or composi-tion of the larger quantity.3.2.7 sample tests, na group of samples (one or more).1This test method is under the jurisdiction of ASTM Committee D01 on Paintand Related Coatings, Materials, and Applications and is the direct responsibility ofSubcommittee D01.28 on Biodeterioration.Curren
14、t edition approved Oct. 1, 2013. Published November 2013. DOI:10.1520/D7855_D7855M13.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary
15、page onthe ASTM website.Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States13.2.8 test run, nthe evaluation of coated building productsin accordance with the procedure outlined in this test method.3.2.9 test specimen, na portion of a test
16、 unit needed toobtain a single test determination.4. Summary of Test Method4.1 This test method is an indirect inoculation to a coatedinterior building product of two fungal organisms, Aspergillusniger and Penicillium citrinum. Test specimens are placed in anenvironmental chamber maintained at 30 6
17、2C 86 6 3.6Fand at greater than 90 % relative humidity for four weeks.Humidity is maintained by adding sufficient sterile DI water tothe bottom of the covered test chamber. A continuous fungalinoculation is provided by open Petri dishes supporting sevenday cultures of the two test organisms placed o
18、n a rack belowthe test pieces. Test specimens are removed from the chamberafter four weeks exposure and examined for fungal growth.The evaluation is a macroscopic inspection of the test pieceswith indirect lighting.5. Significance and Use5.1 An accelerated test for determining the resistance ofinter
19、ior coated building products to mold growth is useful inestimating the relative performance for use in interior environ-ments under conditions favorable to fungal growth.5.2 Static or environmental chambers provide controlledlaboratory micro-environment conditions. These chambers arenot intended to
20、duplicate room conditions, and care must betaken when interpreting the results. Static chambers are not asubstitute for dynamic chambers or field studies.6. Interferences6.1 Proper lab ventilation, hygiene, and aseptic techniquemust be followed to ensure fungal cultures are pure and nocross contamin
21、ation of fungal strains or growth media occurs.6.2 The exposure of test specimens to environmental con-ditions including temperature, humidity, and light can impacttest results. To minimize variability of test results consistenthandling and storage of test specimens is important.7. Apparatus7.1 Envi
22、ronmental ChamberA non-corrosive covered boxcontaining standing water placed in an incubator at 30 6 2C86 6 3.6F will expose the test specimens to a controlledenvironment of temperature and humidity. Containers foundsuitable include glass, polycarbonate3or other plastic storagecontainers which are g
23、enerally available. For example anineteen liter container measuring approximately 460 mm longby 300 mm wide by 230 mm high 18 in. long by 12 in. wideby 9 in. high can accommodate fifteen 75 by 100 mm 3 by 4in. test specimens suspended from rods using cable ties.Opaque chambers shall have a viewing p
24、ort that permitsobservation of chamber controls. Chamber shall permit tem-perature and humidity monitoring without opening the cham-ber lid. Examples would include wireless or wired probes.7.1.1 The test chamber shall be designed so that no conden-sate forming on the top interior surface will drip o
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