ACADEMIA SINICAIBMS.ppt

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1、ACADEMIA SINICA IBMS,Specialty: Molecular & Cell Biology Hematopoiesis Apoptosis Regulation,嚴仲陽 美國德州貝勒醫學院 博士,Method Yeast Two-hybrid,Material,Yeast strain：L40 (-his、-trp、-leu、-ade) Plasmid：pBTM116 pACT2 Competent Cell：HB101 Yeast DNA purification kit E coli. DNA purification kit,Result,No Result ,No

2、rmal Transcription,Yeast Two-hybrid Transcription,Plasmid construction,Bait Prey,BaitpBTM116,PreypACT2,Yeast Two-Hybrid,Transformation Assay -Trp, -Leu, -His,-galactosidase assay,gs3955 library,Yeast Transformation Pick colonies Selection three times -galactosidase assay Yeast DNA purification Transform to HB101 Bgl check Retransform to yeast Sequencing,My summer vacation,Experiment Travel Camp,