DB45 T 2172-2020 甘蔗黑穗病的鉴定方法 PCR法.pdf

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1、g3 g3 g44g38g54g3g25g24g17g19g21g19g17g21g19g3 g37g3g20g25g3 g3g3g3g3g3g3 DB45 g5301g16309g3876g7173g14368g8945g2416g3430g7151g7741g2044g3 g39g37g3g23g24g18g55g3g21g20g26g21g258g21g19g21g19g3 g3g3g3g3g3g3 g11086g15181g21767g12429g11259g11450g18602g4560g7151g8971g3g51g38g53g8971g3 g48g72g87g75g82g71g

2、3g82g73g3g51g38g53g3g80g72g87g75g82g71g3g73g82g85g3g71g72g87g72g70g87g76g82g81g3g82g73g3g86g88g74g68g85g70g68g81g72g3g86g80g88g87g3 g3 g3 g3 g3 g21g19g21g19g3g16g3g20g19g3g16g3g21g28g2567g5177g3 g21g19g21g19g3g16g3g20g20g3g16g3g22g19g4564g7155 g5301g16309g3876g7173g14368g8945g2416g5176g3440g11527g11

3、673g12759g10812g4726g3g3g3 g2567g5177 g3 g39g37g23g24g18g55g3g21g20g26g21g178g21g19g21g19g3 g44g3 g11556g3 g3 g8535g3 g2173g3g16432g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g1

4、7g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g44g44g3 g20g3g14643g3364g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17

5、g17g17g17g17g17g17g17g17g17g17g17g3g20g3 g21g3g16372g14643g5719g5445g11096g7095g1318g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g20g3 g2

6、2g3g7519g16925g2748g4554g1145g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g20g3 g23g3g13657g11157g16925g3g17g17g17g17g17g17g1

7、7g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g20g3 g24g3g2511g10806g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17

8、g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g21g3 g25g3g7552g7113g2748g16901g2162g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g

9、17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g21g3 g26g3g1306g3224g2748g16878g3895g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g1

10、7g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g22g3 g27g3g5445g10393g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17

11、g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g22g3 g28g3g6909g1420g8597g20692g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17

12、g3g22g3 g20g19g3g13571g7628g2132g4554g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g23g3 g19572g5509g36g708g17268g7113g5719

13、g709g3g7783g2801g7920g9083g11109g8995g3374g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g24g3 g3 g39g37g23g24g18g55g3g21g20g26g21g178g21g19g21g19g3 g44g44g44g3 g2179g3g3g16438g3 g7

14、516g7095g1318g6457g10135g42g37g18g55g3g20g17g20g252g21g19g21g19g457g7735g2038g2374g5141g1420g4652g2121g3g3g12636g20g18200g2102g726g7735g2038g2374g7095g1318g11444g13571g7604g2748g17319g14713g16372g2121g458g11444g16372g4554 g17319g14713g452g3 g7516g7095g1318g11105g5295g16303g3870g7167g14362g8939g2410g

15、2102g7616g9083g16901g11844g12454g1117g5619g6656g2090g5286g4563g17247g452g3 g7516g7095g1318g11105g5295g16303g13062g1098g7735g2038g2374g6320g7519g4100g2696g1354g5506g2579g452g3 g7516g7095g1318g17319g14713g2437g1405g726g5295g16303g11514g16993g7920g9083g6320g7519g7481g19584g1948g2600g451g5295g16303g2442

16、g9083g7920g9083g6320g7519g7485g2257g7481g19584g1948g2600g451g5295g16303g2617g19264g4537g1944g6320 g7519g7481g19584g1948g2600g451g5295g16303g3870g7167g14362g8939g2410g2102g7616g9083g16901g11844g12454g1117g5619g452g3 g7516g7095g1318g1131g16305g17319g14713g1258g726g7857g1530g451g14591g1133g7861g451g212

17、0g4536g5415g451g21961g7338g13828g451g21748g7235g2324g451g21748g4671g4175g451g2120g7480g1100g451g2120g5143g20161g451g7576g6495g4298g451 g21748g14563g451g7521g10681g17934g451g19990g12272g1952g452g3 g39g37g23g24g18g55g3g21g20g26g21g178g21g19g21g19g3 g20g3 g11086g15181g21767g12429g11259g11450g18602g4560

18、g7151g8971g3g51g38g53g8971g3 g20g3 g14649g3370g3 g7516g7095g1318g16372g4554g1206g11080g15175g21761g12423g11253g11444g51g38g53g7920g9083g7145g8965g452g3 g7516g7095g1318g17970g11096g1214g11080g15175g13556g3625g14599g451g12285g14599g451g12285g14654g2554g3927g11104g7997g7770g21761g12423g11253g14844g1144

19、4g2102g4480g7920g9083g2554g2132g4554g452g3 g21g3 g16378g14649g5725g5451g11102g7101g1324g3 g1083g2119g7095g1318g1117g11444g1973g4585g17994g17911g7095g1117g11444g16372g14643g5719g5445g11096g13884g7604g6208g7516g7095g1318g5621g1085g2591g4673g11444g7569g8558g452g1958g1117g712g8984g7189g7503g11444g5445g1

20、1096g7095g1318g712 g1269g16917g7189g7503g4649g5316g11444g10360g7516g17970g11096g1214g7516g7095g1318g727g1085g8984g7189g7503g11444g5445g11096g7095g1318g712g1958g7472g7136g10360g7516g708g2357g6428g6256g7481g11444g1566g7017g2437g709g17970g11096g1214g7516 g7095g1318g452g3 g42g37g18g55g3g25g25g27g21g3g3g

21、2102g7616g4558g20668g4564g11096g8804g16372g7788g2748g16901g20668g7145g8965g3 g42g37g3g20g28g23g27g28g3g3g4558g20668g4564g11087g10393g4537g1944g17994g11096g16305g8818g3 g22g3 g7525g16931g2754g4560g1151g3 g1083g2119g7519g16925g2748g4554g1145g17970g11096g1214g7516g7095g1318g452g3 g22g17g20g3 g3 g11086g

22、15181g21767g12429g11259g3 g11080g15175g21761g12423g11253g7263g1072g12285g7326g18045g4488g3416g1214g3927g3914g7072g7997g15175g3373g4582g2748g3424g2410g11444g1131g16305g11080g15175g11253g11253g4579g712g11253g2511g14844g7263g11599g14844g1117g11444g6389g4480g14844 g19480g21761g12985g14844g4750g11080g151

23、75g19933g21761g12985g14844g712g11080g15175g21761g12423g11253g1615g8772g9073g451g19736g8804g451g9852g9401g8804g451g1258g1211g9067g2264g12665g3416g11104g19492g17931g15996g1360g6877g6297g7059g712g9128g7683 g11080g15175g14573g1086g2102g15430g712g1239g11087g1072g7569g21761g14498g12423g10470g10393g712g180

24、00g6208g11080g15175g1239g18431g2748g2651g13062g18431g3927g5237g19581g1406g452g3 g22g17g21g3 g3 g13968g2622g18348g19252g5445g2563g5322g948g51g38g53g949g3 g51g38g53g2557g5316g708g51g82g79g92g80g72g85g86g72g3g38g75g68g76g81g3g53g72g68g70g87g76g82g81g15g3g13962g2616g18342g19246g5439g2557g5316g709g7263g3

25、416g2568g5719g451g17968g9883g451g5414g1384g1081g1114g9305g5334g1083g712g2137 g11096g2437g19246g4625g7784g14631g18344g5445g10393g4649g10409g5426g39g49g36g10359g8677g17931g15996g1411g3910g5659g17999g6297g3790g712g3416g11805g7206g19492g1973g1455g11550g11444g10359g8677g11444g6297g3790g18431g17902g2144g7

26、072g2419g1079g1597g712 g1278g7601g5598g18431g11444g39g49g36g17319g4091g712g6297g3790g2090g996g74g13527g11444g51g38g53g1239g10393g452g3 g23g3 g13663g11163g16931g3 g1083g2119g13657g11157g16925g17970g11096g1214g7516g7095g1318g452g3 g51g38g53g726g13962g2616g18342g19246g5439g2557g5316g708g51g82g79g92g80g

27、72g85g68g86g72g3g38g75g68g76g81g3g53g72g68g70g87g76g82g81g709g727g3 g39g49g36g726g14177g8791g7784g13062g7784g18344g708g39g72g82g91g92g85g76g69g82g81g88g70g79g72g76g70g3g68g70g76g71g709g727g3 g71g49g55g51g726g14177g8791g7784g13062g7784g14631g1081g12071g18344g708g39g72g82g91g92g16g85g76g69g82g81g88g70

28、g79g72g82g86g76g71g72g3g87g85g76g83g75g82g86g83g75g68g87g72g709g452g3 g3 g39g37g23g24g18g55g3g21g20g26g21g178g21g19g21g19g3 g21g3 g24g3 g2517g10812g3 g11080g15175g21761g12423g11253g11253g2511g14844g1239g11087g4498g4480g712g2102g5171g3416g11080g15175g7997g7770g1973g3910g712g17994g17911g6656g2566g1108

29、0g15175g21761g12423g11253g4498g4480g11444g39g49g36g712g16878g16849g10409g4554g5445 g10393g712g2137g11096g51g38g53g7920g9083g6320g7519g4649g11080g15175g21761g12423g11253g39g49g36g17931g15996g1411g3910g6297g3790g7920g9083g712g2591g5575g2144g2038g11934g11444g18596g4554g13571g7628g452g3 g25g3 g7558g7119

30、g2754g16907g2168g3 g19604g2582g7481g16932g7230g3910g712g7516g7095g1318g1117g1455g11096g2102g7616g13535g708g2651g709g1301g1082g16901g2162g712g11096g8804g12630g2616g42g37g18g55g3g25g25g27g21g16372g4554g11444g1212g13527g8804g712g9145g2554g51g38g53 g11096g8804g16305g8818g17902g2144g1072g13527g8804g6455g

31、7735g452g3 g25g17g20g3 g16907g2168g3 g25g17g20g17g20g3 g1081g13775g11106g3626g8792g3626g11106g10023g708g38g23g43g20g20g49g50g22g709g726g13535g5334g730g28g28g8g452g3 g25g17g20g17g21g3 g1161g1212g14122g3339g1161g18344g1212g19152g708g40g39g55g36g103g21g49g68g709g726g13535g5334g305g28g28g17g19g8g452g3 g

32、25g17g20g17g22g3 g11948g18344g708g43g22g37g50g22g709g726g178g32g20g17g23g22 g3 g74g18g70g80g994g712g2651g18431g305g28g28g17g24g8g452g3 g25g17g20g17g23g3 g2417g1949g10023g3626g1081g11106g3626g9444g2374g19237g708g38g55g36g37g709g726g178g32g20g17g22g21 g3 g74g18g80g47g712g13535g5334g305g28g28g17g19g8g4

33、52g3 g25g17g20g17g24g3 g8799g2374g19152g708g49g68g38g79g709g726g178g32g21g17g20g25g24 g3 g74g18g70g80g994g712g2651g18431g305g28g28g17g24g8g452g3 g25g17g20g17g25g3 g10860g14130g13062g452g3 g25g17g20g17g26g3 g42g72g79g16g53g72g71g7784g18344g7683g7113g452g3 g25g17g20g17g27g3 g9186g8798g708g49g21g709g72

34、6g178g32g19g17g27g20 g3 g74g18g70g80g994g452g3 g25g17g20g17g28g3 g1081g8799g11106g10023g708g38g43g38g79g22g709g726g178g32g20g17g24g19 g3 g74g18g70g80g994g452g3 g25g17g20g17g20g19g3g7184g8804g1161g18359g708g38g43g22g38g43g21g50g43g709g726g178g32g19g17g26g28 g3 g74g18g70g80g994g712g2651g18431g305g28g2

35、8g17g26g8g452g3 g25g17g20g17g20g20g3g39g49g36g3g2102g4480g18431g7735g2038g708g39g49g36g3g80g68g85g78g72g85g709g726g2591g1301g9269g8074g2410g2102g20g19g19 g3 g69g83g794g25g19g19 g3 g69g83g11444g39g49g36g10359g8677g452g3 g25g17g20g17g20g21g3g21g104g55g68g84g3g51g79g88g86g3g51g38g53g3g20148g9255g16901g

36、2162g726g19g17g20 g3 g56g18g541g47g3g55g68g84g3g51g79g88g86g3g51g82g79g92g80g72g85g68g86g72g451g24g19g19 g3 g541g48g3g71g49g55g51g3g72g68g70g75g451g21g19 g3 g80g48g3 g55g85g76g86g16g43g38g79g11g83g43g32g27g17g22g12g451g20g19g19 g3 g80g48g3g46g38g79g451g22 g3 g80g48g3g48g74g38g79g21g451g12387g4554g21

37、62g451g3790g5482g2162g452g3 g25g17g21g3 g16907g2168g18307g2156g3 g25g17g21g17g20g3 g24g238g55g37g40g13641g2024g9192g3 g12320g2566g24g17g23g3g74g1081g13775g11106g3626g8792g3626g11106g10023g708g25g17g20g17g20g709g451g19g17g22g26g21g3g74g1161g1212g14122g3339g1161g18344g1212g19152g708g25g17g20g17g21g709

38、g2748g21g17g26g24g3g74g11948g18344g708g25g17g20g17g22g709 g9446g1214g26g19 g3 g80g47g13535g8804g1117g712g4554g4585g14371g20g19g19 g3 g80g47g712g3416g20g19g22g17g23 g3 g78g51g68g708g20g21g20g263g709g7569g1318g1083g9885g14844g21g19 g3 g80g76g81g452g3 g25g17g21g17g21g3 g19g17g24g238g55g37g40g13641g2024

39、g9192g3 g18431g2566g20g19g19 g3 g80g47g9091g5334g24g104g55g37g40g13635g2018g9186g708g25g17g21g17g20g709g1214g10007g7583g1117g712g2256g1258g28g19g19 g3 g80g47g13535g8804g9255g2352g452g3 g25g17g21g17g22g3 g38g55g36g37g6662g2572g9452g9192g3 g12320g2566g23g3g74g2417g1949g10023g3626g1081g11106g3626g9444g

40、2374g19237g708g25g17g20g17g23g709g451g20g25g17g22g27g3g74g8799g2374g19152g708g25g17g20g17g24g709g451g20g17g21g20g3g74g1081g13775g11106g3626g8792g3626g11106g10023g708g25g17g20g17g20g709g451 g20g17g23g28 g3 g74g1161g1212g14122g3339g1161g18344g1212g19152g708g25g17g20g17g21g709g9446g1214g26g19 g3 g80g47

41、g13535g8804g712g4554g4585g14371g21g19g19 g3 g80g47g712g3416g20g19g22g17g23 g3 g78g51g68g708g20g21g20g263g709g7569g1318g1083g9885g14844g21g19g3 g80g76g81g452g3 g25g17g21g17g23g3 g7190g14850g13541g8810g3 g13535g8804g3416g20g19g22g17g23 g3 g78g51g68g708g20g21g20g263g709g7569g1318g1083g9885g14844g21g19

42、g3 g80g76g81g452g3 g25g17g21g17g24g3 g20g8g10866g14136g13068g2067g14124g3 g39g37g23g24g18g55g3g21g20g26g21g178g21g19g21g19g3 g22g3 g12320g2566g20g3g74g10860g14130g13062g708g25g17g20g17g25g709g9446g1214g20g19g19g3g80g47g11444g19g17g24g104g55g37g40g13635g2018g9186g708g25g17g21g17g21g709g712g17970g5507

43、g2256g10013g15805g2374g2622g2256g1941g20g19g3g996g47g3g42g72g79g16g53g72g71 g7784g18344g7683g7113g708g25g17g20g17g26g709g712g2023g2468g14371g25g19g263g1602g1941g6658g4013g7907g4480g11444g2150g14118g8237g1117g712g2023g2468g2061g3370g452g3 g26g3 g1312g3230g2754g16884g3901g3 g26g17g20g3 g11109g4480g392

44、9g5283g726g13038g11934g14371g19g17g20 g3 g80g74g452g3 g26g17g21g3 g5762g9305g8804g9124g19253g452g3g3 g26g17g22g3 g20744g17999g2023g2027g12267g5619g7530g726g7472g20744g20g24 g3 g19g19g19 g3 g85g83g80g452g3 g26g17g23g3 g51g38g53g6297g3790g1306g452g3 g26g17g24g3 g8804g5283g11109g8995g13099g13583g452g3

45、g26g17g25g3 g2061g14118g6208g1791g13099g13583g452g3 g27g3 g5451g10399g3 g8595g2625g5445g10393g726g24g255g16g38g42g38g55g38g55g42g42g55g55g38g36g55g38g36g36g38g42g16g22g255g712g2557g2625g5445g10393g726g24g255g16g55g42g38g55g42g55g38g42g36g55g42g42g36g36g42g42g55g42g55g16g22g255g712g20148g7503g6297g37

46、90 g10359g8677g3927g4671g1130g23g21g19g3g69g83g452g3 g28g3 g6915g1426g8603g20698g3 g28g17g20g3 g7789g2807g3 g18423g19702g6214g17969g7920g11444g11080g15175g19842g2598g10359g1674g4013g7735g16886g712g1214g16g20g27g263g2016g12769g1117g1549g4488g3895g11096g452g3 g28g17g21g3 g39g49g36g11450g6662g2572g3 g2

47、566g28g17g20g1117g17970g18431g11080g15175g2598g10359g11096g14362g7573g8804g2018g9031g5282g2032g712g1981g11096g13535g8804g2018g9031g1108g18045g712g14362g10086g7342g5282g2622g11096g1085g19256g19154g2202g2096g2202g11966g7022g1941 g11844g19173g1117g712g2256g1941g9186g8798g708g25g17g20g17g27g709g11844g12

48、056g6208g12985g7515g10470g712g2102g2566g20g19g19g3g80g74g7022g1941g20g17g24g3g80g47g12267g5619g12753g1117g712g2256g1941g26g24g19g3g996g47g3g38g55g36g37g6656g2566g9446 g9186g708g25g17g21g17g22g709g712g9169g7163g6495g14737g9255g2352g2622g1214g25g24g263g8804g9124g23g24g3g80g76g81g794g25g19g3g80g76g81g7

49、12g7503g19492g8703g19652g20g24g3g80g76g81g20176g1602g12267g5619g12753g9255g2352g727g2256g1941g24g19g19g3 g996g47g11444g1081g8799g11106g10023g708g25g17g20g17g28g709g712g20176g1602g9255g2352g712g20g21 g3 g19g19g19 g3 g85g83g80g3g12267g5619g22g3g80g76g81g712g17820g12331g1082g9269g9186g13526g19g17g24g3g

50、80g47g14371g7136g12267g5619g12753g1117g712g2256g1941 g12665g1411g12319g708g19g17g24g3g80g47g709g13526g23g263g11444g7184g8804g1161g18359g708g25g17g20g17g20g19g709g712g20176g1602g9255g2352g712g1214g16g20g27g263g2016g12769g1117g19849g13726g20 g3 g75g712g20g21 g3 g19g19g19 g3 g85g83g80g3g12267g5619g22g3