DIN EN 12821-2009 Foodstuffs - Determination of vitamin D by high performance liquid chromatography - Measurement of cholecalciferol (D) or ergocalciferol (D) German version EN 128.pdf
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1、August 2009DEUTSCHE NORM English price group 13No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 67.050!$Y(j“1540571www.
2、din.deDDIN EN 12821Foodstuffs Determination of vitamin D by high performance liquidchromatography Measurement of cholecalciferol (D3) or ergocalciferol(D2)English version of DIN EN 12821:2009-08Lebensmittel Bestimmung von Vitamin D mit Hochleistungs-Flssigchromatographie Bestimmung von Cholecalcifer
3、ol (D3) oder Ergocalciferol (D2)Englische Fassung DIN EN 12821:2009-08SupersedesDIN EN 12821:2000-07www.beuth.deDocument comprises pages26DIN EN 12821:2009-08 National foreword This standard has been prepared by Technical Committee CEN/TC 275 “Food analysis Horizontal methods” (Secretariat: DIN, Ger
4、many). Preliminary work was done by Working Group WG 9 “Vitamins and Carotenoids”. The responsible German body involved in its preparation was the Normenausschuss Lebensmittel und landwirtschaftliche Produkte (Food and Agricultural Products Standards Committee), Technical Committee NA 057-01-13 AA V
5、itamine und Carotinoide. The DIN Standard corresponding to the International Standard referred to in this document is as follows: EN ISO 3696 DIN IS0 3696 Amendments This standard differs from DIN EN 12821:2000-07 as follows: a) The title of the standard has been modified: “Measurement of cholecalci
6、ferol (D3) or ergocalciferol (D2)” instead of “Measurement of cholecalciferol (D3) and ergocalciferol (D2)”. b) The normative reference to EN ISO 5555 has been deleted. c) The validation data for the method based on external standard have been deleted. d) Specifications relating to the validated foo
7、dstuffs have been rendered more precise. e) Specifications relating to the reagents have been given in more detail. f) The figures have been updated. g) The text of this standard has been editorially revised. h) The new rules for drawing up European Standards have been taken into consideration. Prev
8、ious editions DIN EN 12821: 2000-07 National Annex NA (informative) Bibliography DIN ISO 3696, Water for analytical laboratory use Specification and test methods 2 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 12821 April 2009 ICS 67.050 Supersedes EN 12821:2000 English Version Foodstuffs - D
9、etermination of vitamin D by high performance liquid chromatography - Measurement of cholecalciferol (D3) or ergocalciferol (D2) Produits alimentaires - Dosage de la vitamine D par chromatographie liquide haute performance - Dosage du cholcalcifrol (D3) de l ergocalcifrol (D2) ouLebensmittel - Besti
10、mmung von Vitamin D mit Hochleistungs-Flssigchromatographie - Bestimmung von Cholecalciferol (D3) oder Ergocalciferol (D2) This European Standard was approved by CEN on 21 February 2009. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for givi
11、ng this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official ver
12、sions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgiu
13、m, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR S
14、TANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 12821:2009: EEN 12821:2009 (E) 2 Contents Page F
15、oreword 3 1 Scope 4 2 Normative references 4 3 Principle 4 4 Reagents .4 5 Apparatus .8 6 Procedure .9 7 Calculation . 11 8 Precision 12 9 Test report . 13 Annex A (informative) Examples of suitable HPLC systems . 14 Annex B (informative) Examples of suitable extraction and saponification conditions
16、 . 15 Annex C (normative) Examples of suitable semi-preparative and analytical HPLC chromatograms . 16 Annex D (informative) Precision data . 18 Annex E (informative) Additional cleanup step for the determination of vitamin D with use of preparative TLC, column chromatography and or SPE 20 Bibliogra
17、phy . 24 DIN EN 12821:2009-08 EN 12821:2009 (E) 3 Foreword This document (EN 12821:2009) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either
18、by publication of an identical text or by endorsement, at the latest by October 2009, and conflicting national standards shall be withdrawn at the latest by October 2009. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or
19、CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 12821:2000. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belg
20、ium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. DIN EN 12821:2009-
21、08 EN 12821:2009 (E) 4 1 Scope This European Standard specifies a method for the determination of vitamin D3(cholecalciferol) or vitamin D2(ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC). Vitamin D3is primary in foodstuffs of animal origin, while vitamin D2is primary
22、in wild mushrooms. Both vitamin D3 and vitamin D2can be present in fortified foodstuffs. This European Standard is not applicable for samples with a content of vitamin D3and vitamin D2. Apart from the vitamin D activity from the parent forms, vitamin D3and vitamin D2, the corresponding metabolites 2
23、5-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. This European Standard does only include measurement of vitamin D3or vitamin D2. This European Standard provides the base for the analytical methods. It is intended to serve as a frame in which the analyst ca
24、n define his own analytical work in accordance to the standard procedure. This method has been validated in inter-laboratory tests on fortified and non-fortified samples such as margarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4 g/100 g
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