BS ISO 23893-3-2013 Water quality Biochemical and physiological measurements on fish Determination of vitellogenin《水质 鱼的生物化学和生理测量 卵黄蛋白原的测定》.pdf
《BS ISO 23893-3-2013 Water quality Biochemical and physiological measurements on fish Determination of vitellogenin《水质 鱼的生物化学和生理测量 卵黄蛋白原的测定》.pdf》由会员分享,可在线阅读,更多相关《BS ISO 23893-3-2013 Water quality Biochemical and physiological measurements on fish Determination of vitellogenin《水质 鱼的生物化学和生理测量 卵黄蛋白原的测定》.pdf(32页珍藏版)》请在麦多课文档分享上搜索。
1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 23893-3:2013Water quality Biochemicaland physiologicalmeasurements on fishPart 3: Determination of vitellogeninBS ISO 23893-3:2013 BRITISH STANDARDNational forewordThis Br
2、itish Standard is the UK implementation of ISO 23893-3:2013.The UK participation in its preparation was entrusted to TechnicalCommittee EH/3/5, Biological Methods.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to inc
3、lude all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2013. Published by BSI StandardsLimited 2013ISBN 978 0 580 67754 0ICS 13.060.70Compliance with a British Standard cannot confer immunity fromlegal obligations.This Brit
4、ish Standard was published under the authority of theStandards Policy and Strategy Committee on 30 April 2013.Amendments issued since publicationDate Text affectedBS ISO 23893-3:2013 ISO 2013Water quality Biochemical and physiological measurements on fish Part 3: Determination of vitellogeninQualit
5、de leau Mesurages biochimiques et physiologiques sur poisson Partie 3: Dosage de la vitellognineINTERNATIONAL STANDARDISO23893-3First edition2013-04-01Reference numberISO 23893-3:2013(E)BS ISO 23893-3:2013ISO 23893-3:2013(E)ii ISO 2013 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2013All righ
6、ts reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from ei
7、ther ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 23893-3:2013ISO 23893-3:2013(E) ISO 2013 All rights r
8、eserved iiiContents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 25 Minimum performance criteria 26 Test environment . 27 Reagents 38 Apparatus . 39 Sampling procedure 49.1 Sampling of fish 49.2 Sampling of blood plasma . 49.3 Storage of bloo
9、d plasma samples 510 Analytical procedure 510.1 Preparation of the samples 510.2 Determination of vitellogenin 511 Test report 10Annex A (informative) Examples of results: Fathead minnow sandwich ELISA .12Bibliography .19BS ISO 23893-3:2013ISO 23893-3:2013(E)ForewordISO (the International Organizati
10、on for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established ha
11、s the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.Inter
12、national Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publicati
13、on as an International Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights
14、.ISO 23893-3 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods.ISO 23893 consists of the following parts, under the general title Water quality Biochemical and physiological measurements on fish: Part 1: Sampling of fish, handling and preservation o
15、f samples Part 2: Determination of ethoxyresorufin-O-deethylase (EROD) Technical Specification Part 3: Determination of vitellogeniniv ISO 2013 All rights reservedBS ISO 23893-3:2013ISO 23893-3:2013(E)IntroductionVitellogenin (Vtg) is a large phospholipoglycoprotein produced as the yolk protein prec
16、ursor in the liver of oviparous vertebrates, such as fish. Vtg is secreted from hepatocytes through the secretory pathway, enters the circulation and is taken up by the growing oocyte. Plasma Vtg concentrations are normally an indication of the maturational status of the female fish, for reviews see
17、 References 218. More than a decade ago, several studies demonstrated that male fish caught in rivers and streams had high concentrations of plasma Vtg (e.g. References 1423), caused by chemicals acting like oestrogens present in the environment. Since then, numerous studies have shown the fish Vtg
18、to be a highly responsive biomarker for oestrogenic compounds in both in vitro hepatocyte cell cultures, in vivo aquaria studies, and field studies, for reviews see References 121013162026. Hence, Vtg in fish has become an accepted biomarker of xenooestrogenic and antioestrogenic exposure of chemica
19、ls, effluents, and discharges, and has been proposed in chemical testing, as well as environmental monitoring programmes, e.g. Reference 13.However, recent genetic and immunological analyses have demonstrated a general multiplicity of Vtg forms in fish, References 910. The concentrations of circulat
20、ing Vtg proteins (or Vtg gene transcripts) during oogenesis and their degree of induction by oestrogens appear to vary among species and among different types of Vtg within a species. The kinetics of induction of distinct types of Vtg by oestrogens in fish appears to depend on environmental factors
21、(e.g. water temperature and photoperiod), life history stage, sex, and the concentration and type of oestrogenic compound. Based on these findings, it is important that the Vtg targets in a bioassay for oestrogens in a specific species be demonstrated to be an oestrogen-responsive form, and that the
22、 assay be validated with the species in question before embarking on a monitoring programme, Reference 10.The scientific literature contains a multitude of publications on procedures for determining Vtg in fish samples, using immunoassays. Whereas radioimmunoassays (RIA) were a predominant method in
23、 the 1980s and early 1990s, e.g. References 429, enzyme-linked immunosorbent assays (ELISAs) are the dominating principle today. Both the sandwich and competitive ELISA principles provide sensitive results without the use of radioactive isotopes, and have been successfully applied to determine Vtg l
24、evels in several fish species, e.g. References 368121517192122242527283132.This part of ISO 23893 presents a generalized protocol for both the sandwich and competitive ELISA for use in quantification of Vtg in fish blood plasma samples. The application of standardized methods is strongly advised wit
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