KS I ISO 14238-2008 Soil quality-Biological methods-Determination of nitrogen mineralization and nitrification in soils and the influence of chemicals on these processes《土壤质量 生物检测法.pdf

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1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 14238 ： KS I ISO 14238:2008 2008 11 10 http:/www.kats.go.krKS I ISO 14238:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：1998 12 28 ：2008 11 10 2008-0751 ： ： ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 14238:2008 ： Soil quality Biol

2、ogical methods Determination of nitrogen mineralization and nitrification in soils and the influence of chemicals on these processes 1997 1 ISO 14238, Soil quality Biological methods Determination of nitrogen mineralization and nitrification in soils and the influence of chemicals on these processes

3、 , . 1 . , . , . 2 . . ( ) . KS M ISO 10381 6, ( , ) KS M ISO 10390, pH KS M ISO 10694, ( ) KS M ISO 11260, KS M ISO 11261, ( ) KS M ISO 11274, ( ) KS M ISO 11277, ( , KS I ISO 14238:2008 2 ) KS M ISO 11465, ( ) 3 . 3.1 (N ) , 3.2 3.3 3.4 (lD%) . ID25 ID50 25 % 50 % . 4 , . , . 5 5.1 5.1.1 5.1.1.1 ,

4、 1 . 5.1.1.2 KS I ISO 14238:2008 3 ( ) (0.5 %1.5 %) ( ) . . pH 5 . 5.1.2 , , KS M ISO 10381 6 . ( ) 5.1.3 . a) KS M ISO 11277 KS M ISO 11465 KS M ISO 11274 A b) KS M ISO 10390 pH KCl CaCl2 pH KS M ISO 11260 (CEC) KS M ISO 10694 KS M ISO 11261 5.2 5.2.1 0.1 mm0.5 mm . 5.2.2 c(KCl) 1 mol/L 5.2.3 1 kg

5、100 mg . C： N 16： 1 (alfalfa) ( ) , KS I ISO 14238:2008 4 . . (NH4)2SO4 . 5.3 . . . (carrier) . ( ) . (IUPAC) (CAS) / (POW) (pKa) (Koc) 6 . 6.1 6.2 ( .) 6.3 , , 7 7.1 7.1.1 1 , . KS I ISO 14238:2008 5 7.1.2 5 . 28 ( kg NO3mg ) . . , ( ) . 7.2 7.2.1 5.2.3 . . 7.2.2 5.2.3 . C： N . (5.2.3) 6 . 5 ( 3 ).

6、 ( ). . ID25 ID50 . , . a) , . b) , (5.2.1) . , . . 7.3 . a) (bulk) b) ( , 10 g 100 g) . , . . 3 cm . KS I ISO 14238:2008 6 . . . (202) , 5 % (405)(605) % 0.02 MPa . (202) , . ( , ) . . . . . . . 1 mm . 7.4 7.4.1 . 7.4.2 (7.2 ) (0 ) 28 (7.3 ) . , , , 28 . 28 ID25 ID50 . 3 . 7.5 (5.2.2)( 1 g 5 mL) 60

7、 150 rpm (7.4 ) , . . (5.2) . ( 205) 6 . . , . . KS I ISO 14238:2008 7 7.6 , . 8 8.1 , (7.6) (NH4), (NO2) (NO3) kg mg (NH4 N), (NO2 N) (NO3 N) . , “Nmin” . Nmin . Nmin (NH4 NT) (NO2, NT) (NO3, NT) (NH4 NS) (NO2NS) (NO3NS) (NH4 NT) ： ( mg/ kg) (NO2 NT) ： ( mg/ kg) (NO3 NT) ： ( mg/ kg) (NH4 NS) ： ( mg

8、/ kg) (NO2 NS) ： ( mg/ kg) (NO3 NS) ： ( mg/ kg) 8.2 28 ( , NO3 mg/ kg) . kg NO3(mg) kg NO3 N(mg) . . kg NO3 mg 100 kg NO3 mg 100 . a) 1 . 1 ID25 ID50 kg 1 mg kg 10 mg . b) . , . . . KS I ISO 14238:2008 8 9 . a) b) (5.1.2 5.1.3 ) c) ( )(5.3 ) d) , , , , , ( ), ( ), (7.17.6 ) e) , f) , , g) h) ( ) 1 N

9、O3 10080605040252000 0.1 1 ID2510 ID50100 1 000 ( mg/kg) NO3(% ) KS I ISO 14238:2008 9 A ( ) A.1 . A.2 . 105 . A.3 A.3.1 50 mm150 mm, 50 mm100 mm , . A.3.2 . A.3.3 20 mm50 mm . A.3.4 (1052) . A.3.5 0.01 g . A.4 (A.3.1) . . 2 . 1 . (A.3.3) 224 . 105 . KS I ISO 14238:2008 10 A.5 (WHC) . 100WHCddts=mmm

10、m ms ： (g) mt ： ( )(g) md ： (g) A.6 (WHC) . KS I ISO 14238:2008 11 B ( ) 1 KS M ISO 11266, - 2 ANDERSCH, I. and ANDERSON, J.P.E(1991), Influence of pesticides on nitrogen transformation in soil. Toxicological and Environmental Chemistry, 30, pp. 153 158, 1991 3 BREMMER, J.M. Nitrogen availability in

11、dexes. In C.A. BLACK： Methods of soil analysis Part 2：Amer. Soc. Agron. Madison, WI 53711, USA, pp.1324 1345, 1965 4 HENRICKSON, A. and SELMER OLSEN, A.R. Automatic methods for determining nitrate and nitrite in water and soil extracts. Analyst. 95, pp.514 518, 1970 5 SELMER OLSEN, A.R.(1971). Deter

12、mination of ammonium in soil extracts by automated indophenol method. Analyst, 96, pp. 565 568 6 STANFORD, G. and SMITH, S.J. Nitrogen mineralization potential of soils. Soil Sci. Soc. Am. Proc. 36, pp. 445 472, 1972 ： 135513 7017 (02)60094114 (02)600948878 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 14238:2008 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Soil qualityBiological methods Determination of nitrogen mineralization and nitrification in soils and the influence of chemicals on these processes ICS 13.080