EN 15850-2010 en Foodstuffs - Determination of zearalenone in maize based baby food barley flour maize flour polenta wheat flour and cereal based foods for infants and young childr.pdf
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1、BS EN 15850:2010ICS 67.060NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDFoodstuffs Determination ofzearalenone in maizebased baby food,barley flour, maizeflour, polenta, wheatflour and cereal basedfoods for infantsand young children HPLC methodwith immunoaffin
2、itycolumn cleanup andfluorescence detectionThis British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May2010 BSI 2010ISBN 978 0 580 64288 3Amendments/corrigenda issued since publicationDate CommentsBS EN 15850:2010National forewordThis British Stand
3、ard is the UK implementation of EN 15850:2010. The UK participation in its preparation was entrusted to TechnicalCommittee AW/-/3, Food analysis - Horizontal methods.A list of organizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to
4、include all the necessary provisionsof a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunityfrom legal obligations.BS EN 15850:2010EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 15850 April 2010 ICS 67.060 English Version Foods
5、tuffs - Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection Produits alimentaires - Dosage de la zaralnone dans la fari
6、ne dorge, de mas et de bl, la polenta et les produits pour nourrissons et jeunes enfants base de crales - Mthode par chromatographie liquide haute performance avec purification sur colonne dimmunoaffinit et dtection par fluorescence Lebensmittel - Bestimmung von Zearalenon in Suglingsnahrung auf Mai
7、sbasis, Gerstenmehl, Maismehl, Maisgrie, Weizenmehl und Lebensmittel auf Getreidebasis fr Suglinge und Kleinkinder - HPLC-Verfahren mit Reinigung an einer Immunoaffinittssule und Fluoreszenzdetektion This European Standard was approved by CEN on 27 February 2010. CEN members are bound to comply with
8、 the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Cen
9、tre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the offici
10、al versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slova
11、kia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2010 CEN All rights of exploitation in any form and by any means reserved worldwide
12、for CEN national Members. Ref. No. EN 15850:2010: EBS EN 15850:2010EN 15850:2010 (E) 2 Contents Page Foreword 31 Scope 42 Normative references 43 Principle 44 Reagents .45 Apparatus .76 Procedure .97 HPLC analysis . 108 Calculation . 129 Precision 1210 Test report . 13Annex A (informative) Typical c
13、hromatograms 15Annex B (informative) Precision data . 16Bibliography . 18BS EN 15850:2010EN 15850:2010 (E) 3 Foreword This document (EN 15850:2010) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standar
14、d shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2010, and conflicting national standards shall be withdrawn at the latest by October 2010. Attention is drawn to the possibility that some of the elements of thi
15、s document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document has been prepared under a mandate given to CEN by the European Commission and the European Free Trade Association. WARNING The use of this sta
16、ndard can involve hazardous materials, operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of re
17、gulatory limitations prior to use. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany
18、, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. BS EN 15850:2010EN 15850:2010 (E) 4 1 Scope This European Standard specifies a method for the deter
19、mination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in two inter
20、laboratory studies. The first study was for the analysis of samples of maize based baby food, barley flour, maize flour, polenta and wheat flour ranging from 10 g/kg to 335 g/kg, and the second study was for samples of cereal based foods for infants and young children ranging from 9 g/kg to 44 g/kg.
21、 Further information on validation, see Clause 9 and Annex B. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document
22、(including any amendments) applies. EN ISO 3696:1995, Water for analytical laboratory use Specification and test methods (ISO 3696:1987). 3 Principle A test portion is extracted with aqueous acetonitrile or methanol according to the products analyzed. The extract is then diluted with phosphate buffe
23、red saline (PBS) to give an aqueous extract that is applied to an immunoaffinity column containing antibodies specific for zearalenone. Zearalenone is purified and concentrated on the column and removed from the antibodies using acetonitrile or methanol as eluent. Zearalenone is quantified by revers
24、e-phase high performance liquid chromatography (RP-HPLC) with fluorescence detection. 4 Reagents 4.1 General Use only reagents of recognised analytical grade and water complying with grade 1 of EN ISO 3696:1995, unless otherwise specified. Solvents shall be of quality for HPLC analysis, unless other
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