ASTM E2274-2009 Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants《评价洗衣卫生消毒剂和灭菌剂的标准试验方法》.pdf
《ASTM E2274-2009 Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants《评价洗衣卫生消毒剂和灭菌剂的标准试验方法》.pdf》由会员分享,可在线阅读,更多相关《ASTM E2274-2009 Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants《评价洗衣卫生消毒剂和灭菌剂的标准试验方法》.pdf(6页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: E2274 09Standard Test Method forEvaluation of Laundry Sanitizers and Disinfectants1This standard is issued under the fixed designation E2274; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revision. A
2、 number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in top-loadingautomatic clothes
3、washing operations. This test method isdesigned predominantly to provide testing with representativevegetative bacteria but can also be designed to accommodatethe testing of fungi and viruses.NOTE 1This test method does not evaluate sanitizing/disinfectantlaundry detergent/additives for use in front
4、-loading, low water volumeautomatic clothes washing operations.1.2 Knowledge of microbiological techniques is requiredfor these procedures.1.3 The values stated in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.NOTE 2In this method, metric units ar
5、e used for all applications,except for distance in which case inches are used.1.4 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and deter
6、mine the applica-bility of regulatory limitations prior to use.2. Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE177 Practice for Use of the Terms Precision and Bias inASTM Test MethodsE691 Practice for Conducting an Interlaboratory Study toDetermine the Precision of a
7、 Test MethodE1054 Test Methods for Evaluation of Inactivators of An-timicrobial Agents2.2 Other Documents:AATCC Test Method 70-1997 Water Repellency; TumbleJar Dynamic Absorption Test3DIS/TSS 13 LaundryAdditivesDisinfection and Sanitiza-tion, U.S. Environmental Protection Agency, Office ofPesticide
8、Programs, April 198043. Terminology3.1 Definitions:3.1.1 active antimicrobial ingredienta substance added toa formulation intended specifically for the inhibition or inac-tivation of microorganisms.3.1.2 antimicrobial agent(s)an active ingredient designedto suppress the growth or action of microorga
9、nisms.3.1.3 carrier count controlprocedure used to determinethe initial number of microorganisms on a fabric carrierfollowing the inoculation and drying procedure.3.1.4 diluentsterile deionized water, sterile distilled wateror sterile synthetic AOAC hard water that may be used toprepare the active t
10、est formulation, vehicle control or productcontrol for use in the test procedure.3.1.5 diluted product solutiontest formulation, vehiclecontrol, or product control diluted to use concentration.3.1.6 neutralizationa process that results in quenching theantimicrobial activity of a test formulation. Th
11、is may beachieved by dilution of the test formulation(s) to reduce theconcentration of the antimicrobials, or through the use ofchemical agents, called neutralizers, to suppress antibacterialactivity.3.1.7 numbers controlin assessing sanitizer level perfor-mance, procedure used to determine the numb
12、er of microor-ganisms remaining on the fabric carriers and in the wash waterfollowing the test procedure in the presence of the diluent. Thismay also be performed using diluent or phosphate bufferdilution water with surfactant.3.1.8 product controla formulation with or without anactive ingredient(s)
13、 used for comparison to the test formula-tion.3.1.9 test formulationa formulation containing an antimi-crobial agent(s).1This test method is under the jurisdiction of ASTM Committee E35 onPesticides and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.15 on Antimicrobia
14、l Agents.Current edition approved Nov. 1, 2009. Published December 2009. Originallyapproved in 2003. Last previous edition approved in 2003 as E2247 03. DOI:10.1520/E2274-09.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For
15、Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Available from American Association of Textile Chemists and Colorists(AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http:/www.aatcc.org.4Available from United States Environm
16、ental Protection Agency (EPA), ArielRios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http:/www.epa.gov.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.3.1.10 vehicle controlthe test formulation without theactive ingredie
17、nt(s) used for comparison to the test formula-tion.3.1.11 wash waterthe liquid contained in the exposurechamber previously exposed to either uninoculated fabric orfabric inoculated with the challenge microorganism.4. Summary of Test Method4.1 Under simulated laundry conditions, sets of inoculatedfab
18、ric swatches are placed into diluted product solution andagitated. After a specified contact time, the wash water and thetest fabric are individually cultured either quantitatively (sani-tizer efficacy) or qualitatively (disinfectant efficacy).NOTE 3See appropriate regulatory guidance document for t
19、he mini-mum number of replicates required to meet a specific claim.5. Significance and Use5.1 The procedure in this test method is used to evaluate theactivity of a test reagent (antimicrobial agent/active ingredient)or formulation in the reduction or complete kill of the bacterialpopulation in fabr
20、ic and wash water following a single wash.6. Apparatus6.1 Colony Counter, any of several types may be used, forexample, Quebec.6.2 Incubator, any incubator that can maintain the optimumtemperature, 62C, for growth of the challenge microorgan-ism(s).6.3 Sterilizer, any suitable steam sterilizer produ
21、cing theconditions of sterility.6.4 Timer (Stop-clock), any device that can be read forminutes and seconds.6.5 Exposure Chamber, container with closure that canwithstand sterilization. Should be large enough to hold a singlestainless steel spindle yet allow diluted product solution tocompletely cont
22、act the entire fabric spindle during the tum-bling period.NOTE 4Standard lids may form a vacuum seal when steam sterilized.To avoid, prior to sterilization place a piece of paper between lid and jar.6.6 Stainless Steel spindles, Spindles are fabricated from asingle continuous piece of stainless stee
23、l wire, (116 in. diameterand bent to contain 3 horizontal extensions, 2 in. longconnected by 2 vertical sections approximately 2 in. long.)They are shaped so that vertical sections form 150 angles, freeends of 2 outer horizontal extensions are sharpened to a point.Use as carrier for wrapping fabric
24、ballast. See Fig. 1.6.7 Agitator, tumbling device to rotate Exposure Chamberthrough 360 vertical orbit of 4 to 8 in. diameter at 45 to 60rpm or comparable tumbling devices such as, launderometer ortumble jar described in AATCC Test Method 70-1997.6.8 Micropipettor (and Pipet Tips), suitable to deliv
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