ASTM E211-1982(2005) Standard Specification for Cover Glasses and Glass Slides for Use in Microscopy《显微镜用盖玻片和载玻片》.pdf
《ASTM E211-1982(2005) Standard Specification for Cover Glasses and Glass Slides for Use in Microscopy《显微镜用盖玻片和载玻片》.pdf》由会员分享,可在线阅读,更多相关《ASTM E211-1982(2005) Standard Specification for Cover Glasses and Glass Slides for Use in Microscopy《显微镜用盖玻片和载玻片》.pdf(3页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: E 211 82 (Reapproved 2005)Standard Specification forCover Glasses and Glass Slides for Use in Microscopy1This standard is issued under the fixed designation E 211; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the ye
2、ar of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification describes glass covers and slides foruse in routine microscopy.2. Glass2.1 Cover GlassGlass
3、shall be colorless, transparentglass, sufficiently free of pits, bubbles, or cloudiness so as notto interfere with end-use performance. Such defects shall beregarded as major defects and limited accordingly. Inspectionshall be by the unaided eye in a good light viewing a singlecover glass at a time.
4、 Refractive index at sodium D line (589.3nm) shall be 1.523 6 0.005.2.2 Glass SlidesGlass slides covered by this specificationshall consist of colorless, transparent glass which may have aslight greenish tint when viewed edgewise. It shall be suffi-ciently free of pits, nicks, bubbles, striae, scrat
5、ches, andcloudiness when observed by the unaided eye so as not toimpair end-use performance. Index of refraction at the sodiumD line (589.3 nm) shall be 1.50 to 1.54.COVER GLASSES3. Dimensions3.1 General UseThe dimensions of cover glass for gen-eral use shall be as follows:3.1.1 Square22 mm on a sid
6、e.3.1.2 Rectangular22 by 30, 22 by 40, 22 by 50, or 22 by60 mm and 24 by 30, 24 by 40, 24 by 50, and 24 by 60 mm.3.1.3 ThicknessThe thickness shall be as follows:Number Thickness, mm1 0.13 to 0.17112A0.16 to 0.192 0.17 to 0.25ASpecified as optimum by most microscope manufacturers.3.2 Limited UseThe
7、following sizes while recognized,find limited use and are generally not as readily available.3.2.1 Square18, 24 and 25 mm on a side.3.2.2 Rectangular24 by 55 mm, 35 by 50 mm, 45 by 50mm, 20 by 26 mm and 20 by 40 mm.3.2.3 Circular18, 22 or 25 mm in diameter.3.2.4 ThicknessThickness Number 0, 0.08 to
8、0.13 mm.3.3 ToleranceTolerance on dimensions other than thick-ness shall be 60.5 mm.3.4 Blood Counting ChambersThe dimensions of coverglasses for blood counting chambers shall be as follows:Length, mmAwidth, mmAThickness, mmB20 21 0.4 or 0.620 26 0.4 or 0.625 28 0.4 or 0.6AThe tolerance on length an
9、d width shall be 61.0 mm.BThe tolerance on thickness shall be 6 0.06 mm.4. Planeness4.1 General UseCover glass may be expected to pass thetest as described in 14.1.4.2 Blood Counting ChambersCover glass shall be planewithin 0.002 mm on both sides and shall pass the planeness testas described in 16.2
10、.5. Corrosion Resistance5.1 While no corrosion tests for cover glasses are herebyrequired, when desired for critical use, use the test in 14.2.GLASS SLIDES6. Dimensions6.1 General Use:6.1.1 Size75.5 by 25.5 6 0.8 mm.6.1.2 Thickness A1.0 6 0.15 mm.Thickness B1.2 6 0.15 mm.6.2 Darkfield and Interferen
11、ce Microscopy:6.2.1 Size75.5 by 25.5 6 0.8 mm.6.2.2 Thickness C1.20 6 0.005 mm.Thickness D0.90 6 0.075 mm.Thickness E1.506 0.05 mm.6.3 Embryology37.5 6 0.8 or 50.56 0.8 by 75.5 6 0.8mm.6.3.1 ThicknessSame as either thickness A or thickness Bin 6.1 on general-use glass slides.6.4 Petrography:6.4.1 Si
12、ze25.5 by 45.5, 27 by 46, or 28 by 48 6 0.8 mm.1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onGlass Apparatus.Current edition approved May 1, 2005. Published October 2005. Originallyapproved in 1963
13、. Last previous edition approved in 1999 as E 211 82(1999)e1.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.6.4.2 ThicknessSame as thickness A or B under general-use slides.7. Planeness and Parallelism7.1 General UseNo requirements.
14、7.2 Darkfield, Interference, Embryology, PetrographyNorequirements.8. Corrosion Resistance8.1 General Use and Darkfield and InterferenceMicroscopyThe slides shall not be adversely affected byboilingnor shall they exhibit any pink color in the solubilitytest.8.2 Embryology, PetrographyNo requirements
15、.9. Workmanship9.1 All edges of all glass slides shall be polished or ground.They shall pass the wettability tests in Section 17. General-usemicroscope slides shall be smooth and free from nicks orblemishes on the short edge which might impair their service-ability for blood smear work.9.2 Frosted E
16、ndOne end of one or both surfaces ofmicroscope slides may be frosted to allow marking with a No.2 pencil or ballpoint pen for a distance of 19 6 3 mm from theend. Alternatively, both ends of the microscope slide may befrosted for a distance of 9 6 2 mm.GENERAL10. Sampling10.1 The cover glass and gla
17、ss slides to be tested forcompliance with these specifications shall be selected atrandom from the lot or shipment. Ten or more cover glasses orslides shall be tested for index of refraction and clarity,resistance to boiling, and solubility.11. Packaging and Package Marking11.1 Cover GlassGeneral-us
18、e cover glass shall be pack-aged in lint-free boxes generally standing on edge so that theglass is convenient to use without contamination. It should beprotected from infiltrating dust and moisture which can causesticking together of the glass.11.2 Glass SlidesSlides shall generally stand on edge in
19、unit packages to present them conveniently for use by the enduser without contamination or loss of cleanliness. Packagesshall be sufficiently lint-free to avoid contamination. Unitpackages may be wrapped in a moisture-proof barrier material,either singly or in groups to reduce field contamination,st
20、icking and to extend shelf life.TEST METHODS12. Index of Refraction12.1 Measure with a refractometer and sodium (yellow)light.13. Clarity13.1 Examine the defects by the unaided eye. This meansthe use of an emmetropic eye or an ametropic eye corrected tonormal vision without further magnification. Ex
21、amine coverglasses in good light in front of a dark surface. Examinegeneral-use slides with daylight or diffuse uniform artificiallight against a uniform dark background. Examine darkfieldand interference microscopy slides with a 103 magnifier in alight beam of at least 25 fc against a black backgro
- 1.请仔细阅读文档,确保文档完整性,对于不预览、不比对内容而直接下载带来的问题本站不予受理。
- 2.下载的文档,不会出现我们的网址水印。
- 3、该文档所得收入(下载+内容+预览)归上传者、原创作者;如果您是本文档原作者,请点此认领!既往收益都归您。
下载文档到电脑,查找使用更方便
5000 积分 0人已下载
下载 | 加入VIP,交流精品资源 |
- 配套讲稿:
如PPT文件的首页显示word图标,表示该PPT已包含配套word讲稿。双击word图标可打开word文档。
- 特殊限制:
部分文档作品中含有的国旗、国徽等图片,仅作为作品整体效果示例展示,禁止商用。设计者仅对作品中独创性部分享有著作权。
- 关 键 词:
- ASTME21119822005STANDARDSPECIFICATIONFORCOVERGLASSESANDGLASSSLIDESFORUSEINMICROSCOPY 显微镜 盖玻片 载玻片 PDF

链接地址:http://www.mydoc123.com/p-527144.html