ASTM D7463-2016 red 8874 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel Fuel Water Mixtures and Fuel Associated Water《燃料 燃料 水混合物和燃料相关水中微生物三.pdf
《ASTM D7463-2016 red 8874 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel Fuel Water Mixtures and Fuel Associated Water《燃料 燃料 水混合物和燃料相关水中微生物三.pdf》由会员分享,可在线阅读,更多相关《ASTM D7463-2016 red 8874 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel Fuel Water Mixtures and Fuel Associated Water《燃料 燃料 水混合物和燃料相关水中微生物三.pdf(10页珍藏版)》请在麦多课文档分享上搜索。
1、Designation: D7463 15D7463 16Standard Test Method forAdenosine Triphosphate (ATP) Content of Microorganismsin Fuel, Fuel/Water Mixtures, and Fuel Associated Water1This standard is issued under the fixed designation D7463; the number immediately following the designation indicates the year oforiginal
2、 adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope*1.1 This test method provides a protocol for capturing, concentrating,
3、 and testing the adenosine triphosphate (ATP) present ina fuel system sub-sample (that is, test specimen) associated with:1.1.1 Microorganisms and hydrophilic particles found in liquid fuels as described in Table X6.1, or1.1.2 Microorganisms and hydrophilic particles found in mixture of fuel and ass
4、ociated bottom water or just associated bottomwater.1.1.3 ATP detected by this bioluminescence test can be derived from cellular ATP, extra-cellular ATP, or some combination ofboth.1.1.4 Cellular and extra-cellularATPutilized to performATPbioluminescence are captured and concentrated from a fuel sys
5、temsample into an aqueous test specimen (that is, sub-sample) for testing. For example, for a fuel system sample that does not containany visible fuel associated bottom water, the aqueous test specimen is the capture solution itself described in 8.2.1.1. For fuelsystem samples that are a mixture of
6、fuel and associated bottom water (that is, free water), the test specimen is an aliquant of thecapture solution and associated bottom water.1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportionalto the concentration of ATP in the samp
7、le. The light is produced and measured quantitatively using dedicated ATP test pens2 anda dedicated luminometer2 and reported in (instrument specific) Relative Light Units.1.3 This test method is equally suitable for use in the laboratory or field.1.4 Although bioluminescence is a reliable and prove
8、n technology, this method does not differentiate ATP from bacteria orfungi.1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 1011 M to 3 108 M which is equivalent to 1 1014 moles/mLto 3 1011 moles/mLfor water samples or capture solution.Ass
9、uming testingon fuel phase is performed on a 500 mL 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 1011 M to2 1014 M.1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.6.1 There is one exceptionRela
10、tive Light Unit (RLU) as defined in 3.1.19.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibilityof the user of this standard to establish appropriate safety and health practices and determine the applicability of regulator
11、ylimitations prior to use.2. Referenced Documents2.1 ASTM Standards:3D396 Specification for Fuel Oils1 This test method is under the jurisdiction ofASTM Committee D02 on Petroleum Products, Liquid Fuels, and Lubricants and is the direct responsibility of SubcommitteeD02.14 on Stability and Cleanline
12、ss of Liquid Fuels.Current edition approved Dec. 1, 2015June 1, 2016. Published January 2016June 2016. Originally approved in 2008. Last previous edition approved in 20142015 asD7463 14a.D7463 15. DOI: 10.1520/D7463-15.10.1520/D7463-16.2 The sole source of supply, repair, recertification, and techni
13、cal support of the apparatus or test pen known to the committee at this time is Merck KGaA, 64271 Darmstadt,Germany (Worldwide) or Fuel Quality Services, Inc., 4584 Cantrell Rd., Flowery Branch, GA 30542 (USA). If you are aware of alternative suppliers, please provide thisinformation to ASTM Interna
14、tional Headquarters. Your comments will receive careful consideration at a meeting of the responsible technical committee,1 which you mayattend.3 For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at serviceastm.org. For Annual Book of ASTM Standardsvo
15、lume information, refer to the standards Document Summary page on the ASTM website.This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Becauseit may not be technically possible to ade
16、quately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current versionof the standard as published by ASTM is to be considered the official document.*A Summary of Changes section appears at the end of this standardCopyright ASTM
17、 International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States1D975 Specification for Diesel Fuel OilsD1655 Specification for Aviation Turbine FuelsD2880 Specification for Gas Turbine Fuel OilsD4012 Test Method for Adenosine Triphosphate (ATP) Content of Microorg
18、anisms in WaterD4175 Terminology Relating to Petroleum Products, Liquid Fuels, and LubricantsD6300 Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and LubricantsD7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel Syst
19、em Components for MicrobiologicalTestingD7467 Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)3. Terminology3.1 Definitions:3.1.1 For definition of terms used in this test method, refer to Terminology D4175.3.1.2 adenosine triphosphate, nmolecule comprised of a purine and three phospha
20、te groups, that serves as the primary energytransport molecule in all biological cells.3.1.3 adenosine monophosphate, nmolecule formed by the removal of two (2) molecules of phosphate (one pyrophosphatemolecule) from ATP.3.1.4 aseptic, adjsterile, free from viable microbiological contamination.3.1.5
21、 bioluminescence, nproduction and emission of light by a living organism as the result of a chemical reaction duringwhich chemical energy is converted to light energy.3.1.6 biomass, nbiological material including any material other than fossil fuels which is or was a living organism orcomponent or p
22、roduct of a living organism.3.1.7 capture solution, naqueous solution of proprietary composition used to capture and concentrate hydrophilic compoundsand particles from liquid fuels.3.1.8 cellular adenosine triphosphate (cellular-ATP), nATP present in whole cells, whether they are living or dead.3.1
23、.8.1 DiscussionCellular-ATP is released upon intentional lysis (rupturing) of microbial cells during the sample preparation process. Microbiallyinfected fluids contain both cellular (cell-associated/cell-bound) and extra-cellular ATP.3.1.9 culturable, adjmicroorganisms that proliferate as indicated
24、by the formation of colonies in or on solid growth media,or the development of turbidity in liquid growth media under specified growth conditions.3.1.10 extracellular ATP, nATP that is not contained inside a cell.3.1.10.1 DiscussionATP is released into the environment when cells die and break open (
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