ASTM D6691-17 Standard Test Method for Determining Aerobic Biodegradation of Plastic Materials in the Marine Environment by a Defined Microbial Consortium or Natural Sea Water Inoculum.pdf
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1、Designation: D6691 17Standard Test Method forDetermining Aerobic Biodegradation of Plastic Materials inthe Marine Environment by a Defined Microbial Consortiumor Natural Sea Water Inoculum1This standard is issued under the fixed designation D6691; the number immediately following the designation ind
2、icates the year oforiginal adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope*1.1 This test method is used to determine th
3、e degree and rateof aerobic biodegradation of plastic materials (including for-mulation additives) exposed to pre-grown population of at leastten aerobic marine microorganisms of known genera or theindigenous population existing in natural seawater. The testmethod is conducted under controlled labor
4、atory conditions.1.2 This test method is designed to index polymer materialsthat are possibly biodegradable, relative to a positive referencematerial, in an aerobic environment.1.3 This test method is applicable to all polymer materialscontaining at least 20 % carbon that are not inhibitory to themi
5、croorganisms present in a marine environment.1.4 The values stated in SI units are to be regarded as thestandard.1.5 There is no known ISO equivalent to this standard.1.6 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of
6、the user of this standard to establish appro-priate safety, health, and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accor-dance with internationally recognized principles on standard-ization establis
7、hed in the Decision on Principles for theDevelopment of International Standards, Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade (TBT) Committee.2. Referenced Documents2.1 ASTM Standards:2D618 Practice for Conditioning Plastics for TestingD883 Terminolog
8、y Relating to PlasticsD1193 Specification for Reagent WaterD2593 Test Method for Butadiene Purity and HydrocarbonImpurities by Gas ChromatographyD4129 Test Method for Total and Organic Carbon in Waterby High Temperature Oxidation and by CoulometricDetection3. Terminology3.1 Definitions of Terms Spec
9、ific to This StandardDefinitions of terms applying to this test method appear inTerminology D883.4. Summary of Test Method4.1 This test method consists of the following:4.1.1 Selecting and characterizing (carbon content, molecu-lar weight) plastic materials for testing,4.1.2 Preparing a uniform inoc
10、ulum of various isolatedmarine microorganisms, or obtaining a natural sea watersample (with added inorganic nutrients) for the test relying onthe microbes present as the inoculum.4.1.3 Exposing the test materials to the inoculum,4.1.4 Using a respirometer to measure the total biogas(CO2) produced as
11、 a function of time, and4.1.5 Assessing the degree of biodegradability.4.2 Biodegradability is assessed by determining the propor-tion of polymer-C converted to biogas-C. The percent oftheoretical gas production, expressed as a fraction of themeasured or theoretical carbon content of the test materi
12、al, isreported as a function of time.5. Significance and Use5.1 The use of plastics aboard ships is on the rise and the useof the sea as a trash dumping site is no longer a possibility;consequently, the disposal of plastic materials while at searemains a major issue. It is possible that biodegradabl
13、e plasticswill help to allay public concern by allowing for the safedisposal of plastic materials at sea. This test method has beendeveloped to assess the rate and degree of aerobic biodegra-dation of plastics exposed to marine microorganisms. Aerobic1This test method is under the jurisdiction of AS
14、TM Committee D20 on Plasticsand is the direct responsibility of Subcommittee D20.96 on EnvironmentallyDegradable Plastics and Biobased Products.Current edition approved Dec. 1, 2017. Published January 2018. Originallyapproved in 2001. Last previous edition approved in 2009 as D6691 - 09. DOI:10.1520
15、/D6691-17.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.*A Summary of Changes section appears at the end of
16、 this standardCopyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelo
17、pment of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.1biodegradation is determined by measuring the amount ofbiogas (carbon dioxide) produced during such an exposure.5.2 It is acceptable to use the degree and
18、rate of aerobicbiodegradability of a plastic under the conditions of this testmethod to estimate the persistence of that plastic in biologi-cally active marine environments, for example, seashore andopen-ocean. However, it shall be recognized that predictinglong-term environmental fate and effects f
19、rom the results ofshort-term exposure to a simulated marine environment isdifficult. Thus, caution shall be exercised when extrapolatingthe results obtained from this or any other controlled-environment test to disposal in the natural environment.6. Apparatus6.1 Aerobic Digestion and Gas Measuring A
20、pparatus:6.1.1 Biogas production can be monitored through the useof any number of respirometry systems. The respirometrysystem must be able to detect low levels of carbon dioxideproduction. A carbon dioxide sensor consisting of a singlebeam, nondispersive infrared device with a maximum mea-surement
21、capability of 1 % carbon dioxide is recommended.6.1.2 Sample Bottles125-mL autoclave bottles withplastic, screw-on lids. The lids shall contain three entry portsfor biogas collection as well as a tetrafluorethylene seal ring.These flasks as well as their lids are supplied by the variousrespirometry
22、companies.6.1.3 All components of the gas-volume measuring andcollection system must be of sufficient quality to prevent gasdiffusion between the system and the surrounding atmosphere.6.2 Water Bath or Controlled-Environment Shaker/Incubator, capable of maintaining the temperature of thedigestion fl
23、asks at 30 6 2C.6.3 Analytical Balance, (60.1 mg), to weigh the test mate-rials.7. Reagents and Materials7.1 All chemicals shall be of American Chemical Society(ACS) reagent-grade quality.7.2 Type IV distilled water shall be prepared in accordancewith Specification D1193.7.3 Marine agar per litre co
24、nsists of the following:Bacto tryptone 5.0 0.1 gBacto yeast extract 2.5 0.1 gBacto dextrose (glucose) 1.0 0.1 gBacto agar 15.0 0.1 g7.4 Marine broth per litre consists of the following:Peptone 5.0 0.1 gYeast extract 1.0 0.1 gFerric citrate 0.1 0.1 gSodium chloride 19.4 0.1 gMagnesium chloride, dried
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