BS EN ISO 16654-2001 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157《食品和动物饲料的微生物学 大肠杆菌0157检测的水平方法》.pdf
《BS EN ISO 16654-2001 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157《食品和动物饲料的微生物学 大肠杆菌0157检测的水平方法》.pdf》由会员分享,可在线阅读,更多相关《BS EN ISO 16654-2001 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157《食品和动物饲料的微生物学 大肠杆菌0157检测的水平方法》.pdf(26页珍藏版)》请在麦多课文档分享上搜索。
1、BRITISH STANDARD BS EN ISO 16654:2001 Incorporating Corrigendum No. 1 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Escherichia coli 0157 The European Standard EN ISO 16654:2001 has the status of a British Standard ICS 07.100.30 NO COPYING WITHOUT BSI PERMISSI
2、ON EXCEPT AS PERMITTED BY COPYRIGHT LAWBS EN ISO 16654:2001 This British Standard, having been prepared under the direction of the Consumer Products and Services Sector Committee, was published under the authority of the Standards Committee and comes into effect on 15 August 2001 BSI 07-2001 ISBN 0
3、580 37719 9 National foreword This British Standard is the official English language version of EN ISO 16654:2001. It is identical with ISO 16654:2001. The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology, which has the responsibility to: A list of organiza
4、tions represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by
5、using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immun
6、ity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them
7、in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN ISO title page, the EN ISO foreword page, the ISO title page, pages ii to v, a blank page, pages 1 to 13, the annex ZA page, an inside back cover and a back cover. The BSI copyright notice displayed in t
8、his document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments 13344 Corrigendum No. 1 July 2001 Incorporation of annex ZAEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 16654 May 2001 ICS 07.100.30 English version Microbiology of food a
9、nd animal feeding stuffs - Horizontal method for the detection of Escherichia coli O 157 (ISO 16654:2001) Microbiologie des aliments - Mthode horizontale pour la recherche des Escherichia coli O 157 (ISO 16654:2001) Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren fr den Na
10、chweis von Escherichia coli O 157 (ISO 16654:2001) This European Standard was approved by CEN on 1 May 2001. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any altera
11、tion. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translati
12、on under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
13、 Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2001 CEN All rights of exploitation in any form and b
14、y any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16654:2001 EForeword The text of the International Standard ISO 16654:2001 has been prepared by Technical Committee ISO/TC 34 “Agricultural food products“ in collaboration with Technical Committee CEN/TC 275 “Food analysis - Ho
15、rizontal methods“, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2001, and conflicting national standards shall be withdrawn at the latest by No
16、vember 2001. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands
17、, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of the International Standard ISO 16654:2001 was approved by CEN as a European Standard without any modification. NOTE: Normative references to International Standards are listed in annex ZA (normative
18、). EN ISO 16654:2001Reference number ISO 16654:2001(E) INTERNATIONAL STANDARD ISO 16654 First edition 2001-05-01 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Escherichia coli O157 Microbiologie des aliments Mthode horizontale pour la recherche des Escherichia
19、 coli O157 EN ISO 16654:2001ii iii Contents Page Foreword.iv Introduction.v 1 Scope 1 2 Normative references 1 3 Term and definition .1 4 Principle2 5 Culture media, reagents and antisera .2 6 Apparatus and glassware .7 7 Sampling.8 8 Preparation of test sample8 9 Procedure (see annex A)8 9.1 Test p
20、ortion and initial suspension .8 9.2 Enrichment .8 9.3 Immunomagnetic separation (IMS)8 9.4 Plating out onto selective agars and identification of E. coli O157 colonies9 9.5 Confirmation.10 9.6 Further characterization11 10 Quality assurance11 10.1 Test strains for quality assurance purposes11 10.2
21、Culture method 11 11 Expression of results 11 12 Test report 11 Annex A (normative) Diagram of procedure 12 Bibliography13 EN ISO 16654:2001 Annex ZA (normative) Normative references to international publications with their relevant European publications.14iv Foreword ISO (the International Organiza
22、tion for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established
23、has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. In
24、ternational Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the
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