BS EN ISO 16649-3-2015 Microbiology of the food chain Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli Detection and most probable number technu.pdf
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1、BS EN ISO 16649-3:2015Microbiology of the foodchain Horizontal methodfor the enumeration ofbeta-glucuronidase-positiveEscherichia coliPart 3: Detection and most probablenumber technique using 5-bromo-4-chloro-3-indolyl-D-glucuronideIncorporating corrigendum February 2017(ISO 16649-3:2015)BSI Standar
2、ds PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS EN ISO 16649-3:2015 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN ISO 16649-3:2015. It supersedes DD ISO/TS 16649-3:2005 which is withdrawn.The UK participation in its preparation was ent
3、rusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British S
4、tandards Institution 2017.Published by BSI Standards Limited 2017ISBN 978 0 580 97525 7 ICS 07.100.30 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 201
5、5.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dImplementation of ISO corrected text 15 December28 February 20172016: See ISO foreword for detailsEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 16649-3 May 2015 ICS 07.100.30 English Version Microbiology of the fo
6、od chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-D-glucuronide (ISO 16649-3:2015, Corrected version 2016-12-15) Microbiologie de la chane alimentaire - Mthode horiz
7、ontale pour le dnombrement des Escherichia coli bta-glucuronidase positive - Partie 3: Recherche et technique du nombre le plus probable utilisant le bromo-5-chloro-4-indolyl-3 -D-glucuronate (ISO 16649-3:2015, Version corrige 2016-12-15) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren
8、zur Zhlung von -Glucuronidase-positiven Escherichia coli - Teil 3: Nachweis und Bestimmung der wahrscheinlichsten Keimzahl unter Verwendung von 5-Brom-4-Chlor-3-Indol-D-Glucuronid (ISO 16649-3:2015, korrigierte Fassung 2016-12-15) This European Standard was approved by CEN on 16 April 2015. CEN memb
9、ers are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on applicati
10、on to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Managem
11、ent Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
12、Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 1
13、7, B-1000 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16649-3:2015 EEN ISO 16649-3:2015 (E) European foreword This document (EN ISO 16649-3:2015) has been prepared by Technical Committee ISO/TC 34 “Food produc
14、ts” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2015
15、, and conflicting national standards shall be withdrawn at the latest by November 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights
16、. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, G
17、ermany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 16649-3:2015, Corrected version 2016-12-15 has bee
18、n approved by CEN as EN ISO 16649-3:2015 without any modification. BS EN ISO 16649-3:2015iiISO 16649-3:2015(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 24 Principle 24.1 Detection method . 24.2 Enumeration method 34.2.1 Inoculation of three or five tubes o
19、f double strength liquid selective enrichment medium 5.3.1.1 a) with an equal volume of the test sample if the initial product is liquid, or with an equal volume of the initial suspension in the case of other products. 35 Dilution fluids and culture media . 35.1 General . 35.2 Dilution fluids 35.3 C
20、ulture media . 35.3.1 Minerals modified glutamate medium (selective enrichment medium) . 45.3.2 Tryptone bile glucoronide agar (second selective enrichment medium) . 55.3.3 Performance testing for the quality assurance of the culture media . 56 Apparatus and glassware 67 Sampling 68 Preparation of t
21、est sample . 79 Procedure. 79.1 Detection method . 79.1.1 Test portion, initial suspension, and dilutions . 79.1.2 Incubation of selective enrichment medium . 79.1.3 Subculturing 79.1.4 Secondary incubation 79.1.5 Examination of the plates . 79.1.6 Interpretation . 79.2 Enumeration method 79.2.1 Tes
22、t portion, initial suspension, and dilutions . 79.2.2 Inoculation of the selective enrichment medium 89.2.3 Incubation 89.2.4 Subculturing 89.2.5 Second incubation 89.2.6 Examination of the plates . 89.2.7 Interpretation . 810 Expression of results 910.1 Detection method . 910.2 Enumeration method 9
23、11 Precision . 912 Test report . 9Bibliography .10 ISO 2015 All rights reserved iiiContents PageBS EN ISO 16649-3:2015ISO 16649-3:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing
24、International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaiso
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