BS EN ISO 11290-1-2017 Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp Detection method《食品和动物饲料的微生物.pdf

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1、Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.Part 1: Detection methodBS EN ISO 11290-1:2017BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE

2、 NORM EN ISO 11290-1 June 2017 ICS 07.100.30 Supersedes EN ISO 11290-1:1996English Version Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method (ISO 11290-1:2017) Microbiologie de la chane alime

3、ntaire - Mthode horizontale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. - Partie 1: Mthode de recherche (ISO 11290-1:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren fr den Nachweis und die Zhlung von Listeria monocytogenes und von Listeria spp

4、. - Teil 1: Nachweisverfahren (ISO 11290-1:2017) This European Standard was approved by CEN on 6 April 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any altera

5、tion. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made

6、by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia

7、, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR

8、 STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11290-1:2017 ENational forewor

9、dThis British Standard is the UK implementation of EN ISO 11290-1:2017. It supersedes BS EN ISO 11290-1:1996+A1:2004, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obtain

10、ed on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limited 2017ISBN 978 0 580 77715 8ICS 07.100.30Compliance with

11、a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 August 2017.Amendments/corrigenda issued since publicationDate Text affectedBRITISH STANDARDBS EN ISO 11290-1:2017EUROPEAN STA

12、NDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11290-1 June 2017 ICS 07.100.30 Supersedes EN ISO 11290-1:1996English Version Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method (ISO 11290-1:2017)

13、 Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. - Partie 1: Mthode de recherche (ISO 11290-1:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren fr den Nachweis und die Zhlung von Listeria mo

14、nocytogenes und von Listeria spp. - Teil 1: Nachweisverfahren (ISO 11290-1:2017) This European Standard was approved by CEN on 6 April 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a nati

15、onal standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A ver

16、sion in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus,

17、Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United

18、 Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN IS

19、O 11290-1:2017 EBS EN ISO 11290-1:2017EN ISO 11290-1:2017 (E) 2 European foreword This document (EN ISO 11290-1:2017) has been prepared by Technical Committee ISO/TC 34 “Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which

20、is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2017, and conflicting national standards shall be withdrawn at the latest by December 2017. Attention is drawn to the possi

21、bility that some of the elements of this document may be the subject of patent rights. CEN shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 11290-1:1996. This document has been prepared under a mandate given to CEN by the European Commissio

22、n and the European Free Trade Association. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former

23、Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 1

24、1290-1:2017 has been approved by CEN as EN ISO 11290-1:2017 without any modification. ISO 2017Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection methodMicrobiologie de la chane alimentaire Mthode horizon

25、tale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. Partie 1: Mthode de rechercheINTERNATIONAL STANDARDISO11290-1Second edition2017-05Reference numberISO 11290-1:2017(E)BS EN ISO 11290-1:2017 ISO 2017Microbiology of the food chain Horizontal method for the detectio

26、n and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection methodMicrobiologie de la chane alimentaire Mthode horizontale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. Partie 1: Mthode de rechercheINTERNATIONAL STANDARDISO11290-1Second edit

27、ion2017-05Reference numberISO 11290-1:2017(E)BS EN ISO 11290-1:2017ISO 11290-1:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in

28、any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Bla

29、ndonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS EN ISO 11290-1:2017ISO 11290-1:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specifi

30、ed, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs

31、 member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgISO 11290-1:2017(E)Foreword vIntroduction vi1 Scope . 12 Normative references 13 Terms and definitions .

32、24 Principle 24.1 General . 24.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth) . 24.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth) 34.4 Plating

33、 out and identification . 34.5 Confirmation . 35 Culture media and reagents 36 Equipment and consumables 37 Sampling 48 Preparation of test sample . 49 Procedure. 49.1 Test portion and initial suspension . 49.2 Primary enrichment . 49.3 Secondary enrichment . 59.4 Plating out and identification . 59

34、.4.1 General 59.4.2 Agar Listeria according to Ottaviani and Agosti . 59.4.3 Second selective medium . 69.5 Confirmation of Listeria monocytogenes or Listeria spp. . 69.5.1 Selection of colonies for confirmation 69.5.2 Confirmation of L. monocytogenes . 69.5.3 Confirmation of Listeria spp. . 109.6 I

35、nterpretation of morphological and physiological properties and of the biochemical reactions 119.7 Additional characterization of isolated strains (optional) .1110 Expression of results .1111 Performance characteristics of the method 1111.1 Method validation studies 1111.2 Sensitivity . 1111.3 Speci

36、ficity . 1111.4 Level of detection (LOD50) . 1112 Test report 1213 Quality assurance .12Annex A (normative) Diagram of procedure .13Annex B (normative) Composition and preparation of culture media and reagents .14Annex C (informative) Distinction of Listeria spp. from other genera .27Annex D (inform

37、ative) Reactions for the identification of Listeria species 28Annex E (informative) Listeria selective agars 30 ISO 2017 All rights reserved iiiContents PageBS EN ISO 11290-1:2017ISO 11290-1:2017(E)Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes .

38、31Bibliography .35iv ISO 2017 All rights reservedBS EN ISO 11290-1:2017ISO 11290-1:2017(E)Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes .31Bibliography .35iv ISO 2017 All rights reserved ISO 11290-1:2017(E)ForewordISO (the International Organiza

39、tion for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established

40、has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Th

41、e procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the e

42、ditorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any paten

43、t rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. Fo

44、r an explanation on the voluntary nature of standards, the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www

45、.iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CEN) Technical Committee CEN/TC 275, Food analysis Horizontal methods, in collaboration with ISO Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology, in accordance with

46、the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This second edition cancels and replaces the first edition (ISO 11290-1:1996), which has been technically revised. It also incorporates the amendment ISO 11290-1:1996/Amd.1:2004.The main changes, compared to ISO 11290-1:19

47、96, are the following. The detection of Listeria monocytogenes has been modified as listed below. Primary enrichment in half-Fraser broth: incubation for 25 h 1 h. Secondary enrichment in Fraser broth: incubation for 24 h 2 h.29 Half-Fraser and Fraser broths may be refrigerated before transfer or is

48、olation on selective agar for a maximum of 72 h. Storage of isolation plates: incubated plates can be refrigerated for a maximum of two days before reading. Microscopic aspect for confirmation is optional if the isolation agar allows distinction between pathogenic and non-pathogenic Listeria spp. CA

49、MP test and catalase test are optional. Inclusion of new performance characteristics. Moreover, detection of Listeria spp. has been included in the scope and the title changed accordingly.A list of parts in the ISO 11290 series can be found on the ISO website. ISO 2017 All rights reserved vBS EN ISO 11290-1:2017ISO 11290-1:2017(E)IntroductionThe main changes, listed in the foreword, introduced in this document compared to ISO 11290-1:1996 are considered as major (see ISO 1746828). The