DB45 T 2031-2019 甘蔗白叶病植原体巢式PCR检测技术规程.pdf

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1、g44g38g54g3 g25g24g17g19g21g19g17g19g20g3 g37g3g20g25g3 g3g3g3g3g3g3 DB45 g5301g16309g3876g7173g14368g8945g2416g3430g7151g7741g2044g3 g39g37g3g23g24g18g55g3g21g19g22g20g258g21g19g20g28g3 g3g3g3g3g3g3 g11086g15181g11443g2604g11259g8003g2517g1417g5144g5445g51g38g53g7926g9089g6326g7525g16378g12353g3 g3

2、9g72g87g72g70g87g76g81g74g3g87g72g70g75g81g76g84g88g72g3g85g72g74g88g79g68g87g76g82g81g86g3g73g82g85g3g83g75g92g87g82g83g79g68g86g80g68g3g82g73g3g54g88g74g68g85g70g68g81g72g3g58g75g76g87g72g3g47g72g68g73g3g69g68g86g72g71g3g82g81g3 g49g72g86g87g72g71g3g51g38g53g3 g3 g3 g3 g21g19g20g28g3g16g3g20g21g3g

3、16g3g19g24g2567g5177g3 g21g19g20g28g3g16g3g20g21g3g16g3g22g19g4564g7155 g5301g16309g3876g7173g14368g8945g2416g5176g3440g11527g11673g12759g10812g4726g3 g3 g3 g2567g5177 g3 g39g37g23g24g18g55g3g21g19g22g20g178g21g19g20g28g3 g44g3 g2179g3 g3 g16438g3 g7516g7735g2038g6457g10135g42g37g18g55g3g20g17g20g17

4、8g21g19g19g28g13577g2090g11444g16372g2121g17319g14713g452g3 g7516g7735g2038g11105g5295g16303g3870g7167g14362g8939g2410g13062g1098g2254g1948g4564g6656g2090g5286g11521g11667g4558g7149g452g3 g7516g7735g2038g11105g5295g16303g13062g1098g7735g2038g2374g6320g7519g4100g2696g1354g5506g2579g452g3 g7516g7735g2

5、038g17319g14713g2437g1405g726g5295g16303g3870g7167g14362g8939g2410g1996g1098g12289g4502g19602g11080g15175g11844g12454g6256g451g5295g16303g11080g15175g18055g1360g7017g14495g18429g9961g4558g20668g4564g452g3 g7516g7735g2038g1131g16305g17319g14713g1258g2696g726g19990g18433g14842g451g4539g1566g21631g451g

6、2120g7236g17849g451g16307g6495g5482g451g5456g4671g12283g451g7550g7576g10894g451g5456g14739g2430g451g7794g5951g1217g451g20863 g7253g10245g451g2120g1133g7039g451g2660g5418g7230g451g20172g7861g7136g451g2120g10944g452g3 g39g37g23g24g18g55g3g21g19g22g20g178g21g19g20g28g3 g20g3 g11086g15181g11443g2604g112

7、59g8003g2517g1417g5144g5445g51g38g53g7926g9089g6326g7525g16378g12353g3 g20g3 g14649g3370g3 g7516g7735g2038g16372g4554g1206g11080g15175g11437g2598g11253g7997g2511g1411g708g54g88g74g68g85g70g68g81g72g3g58g75g76g87g72g3g47g72g68g73g3g51g75g92g87g82g83g79g68g86g80g68g709g5138g5439g51g38g53g7920g9083g114

8、44g2511g10806g451g16901 g2162g451g1131g16305g1306g3224g1086g16878g3895g451g7783g2801g11444g18423g19702g1549g4488g1086g17920g17859g451g7920g9083g7145g8965g2748g13571g7628g6655g17952g2554g2132g4554g452g3 g7516g7735g2038g17970g11096g1214g11080g15175g11437g2598g11253g11253g2511g14844g7997g2511g1411g5445

9、g17319g11444g11253g4579g2554g11253g2511g10393g11444g5659g17999g7920g9083g452g3 g21g3 g16378g14649g5725g5451g11102g7101g1324g3 g1083g2119g7095g1318g4649g1214g7516g7095g1318g11444g5316g11096g7263g5621g1085g2591g4673g11444g452g2065g7263g8984g7189g7503g11444g5445g11096g7095g1318g712g1269g6256g8984g7189g

10、7503g11444g10360g7516g17970g11096g1214g7516g7095 g1318g452g2065g7263g1085g8984g7189g7503g11444g5445g11096g7095g1318g712g1958g7472g7136g10360g7516g708g2357g6428g6256g7481g11444g1566g7017g2437g709g17970g11096g1214g7516g7095g1318g452g3 g42g37g18g55g3g25g25g27g21g178g21g19g19g27g3g3g2102g7616g4558g20668

11、g4564g11096g8804g16372g7788g2748g16901g20668g7145g8965g3 g42g37g18g55g3g21g27g19g25g26g3g3g11080g15175g21748g2598g11253g11253g8706g4558g7206g14743g1913g53g55g16g51g38g53g7920g9083g7145g8965g3 g49g60g18g55g3g21g26g23g22g3g3g11080g15175g11437g14498g7569g13545g11253g14844g7920g20668g7920g11227g6320g751

12、9g16372g12347g3g3g4558g7206g14743g1913g4554g18431g51g38g53g8965g3 g22g3 g2517g10812g3 g5138g5439g51g38g53g11g3g49g72g86g87g72g71g3g51g82g79g92g80g72g85g68g86g72g3g38g75g68g76g81g3g53g72g68g70g87g76g82g81g12g3g7263g1072g12285g2568g5426g11444g13962g2616g18342g19246g2557g5316g708g51g38g53g709g712g1455g

13、11096g1108g4649g708g13884 g19854g1072g4649g709g51g38g53g5445g10393g6297g3790g4540g7076g11444g10359g8677g452g2137g11096g7997g2511g1411g20g25g54g85g53g49g36g3626g3344g5311g2119g17994g11096g5445g10393g51g20g18g51g26g2748g53g20g25g41g21g81g18g53g20g25g53g21g712g1420g1130 g5138g5439g51g38g53g11444g3910g1

14、495g2748g1973g1495g10409g5426g5445g10393g4649g712g2591g11096g1214g11080g15175g11437g2598g11253g7997g2511g1411g11444g5659g17999g7920g9083g452g5138g5439g51g38g53g2591g3790g2256g51g38g53g7920g9083g11444g9893 g7039g5334g2748g2557g5316g10409g5426g5719g452g3 g23g3 g16907g2168g3 g23g17g20g3 g6256g1455g1109

15、6g16901g2162g3447g1130g2102g7616g13535g712g4558g20668g4564g11096g8804g12630g2616g42g37g18g55 g3 g25g25g27g21g178g21g19g19g27g3g11444g1212g13527g8804g6455g7735g712g1958g1117g9145g2554g51g38g53g11444g11096g8804 g5316g12630g2616g1072g13527g8804g6455g7735g452g3 g23g17g21g3 g9186g8798g452g3 g23g17g22g3 g

16、7997g10393g3626g3344g13556g39g49g36g6656g2566g16901g2162g11522g452g3 g23g17g23g3 g42g72g79g3g53g72g71g7784g18344g7683g7113g452g3 g23g17g24g3 g21g238g40g68g86g92g55g68g84g3g51g38g53g3g2557g5316g20148g9255g9186g452g9091g5334g1130g20g19 g3 g80g80g82g79g18g47g712g1958g1117g2651g7481g19g17g19g24 g3 g56g1

17、8g173g47g3g55g68g84g3g39g49g36g3g51g82g79g92g80g72g85g68g86g72g451g19g17g23 g3 g48g80g3g71g49g55g51g3g48g76g91g87g88g85g72g451g23 g3 g80g48g48g74 g21g14 g451g21g238g51g38g53g3g37g88g73g73g72g85g452g3 g23g17g25g3 g20g238g55g40g13635g2018g9186g726g5170g2910g1239g2801g452g3 g23g17g26g3 g20 g3 g24g19g19

18、 g3 g69g83g3g39g49g36g2102g4480g17240g18431g7735g2038g10393g452g3 g23g17g27g3 g10860g14130g13062g712g11109g8995g13527g452g3 g23g17g28g3 g1973g3910g7920g9083g5445g10393g5311g2119g726g3 g68g12g3 g3910g5445g10393g4649g726g3 g20g12g3 g51g20g726g24g254g16g3g36g36g42g36g42g55g55g55g42g36g55g38g38g55g42g42

19、g38g55g38g36g42g42g36g55g55g16g22g255g727g3 g21g12g3 g51g26g726g24g254g16g3g38g42g55g38g38g55g55g38g36g55g38g42g42g38g55g38g55g55g16g22g255g452g3 g39g37g23g24g18g55g3g21g19g22g20g178g21g19g20g28g3 g21g3 g69g12g3 g1973g5445g10393g4649g726g3 g20g12g3 g53g20g25g41g21g81g726g24g254g16g3g42g36g36g36g38g4

20、2g36g38g55g42g38g55g36g36g42g36g38g55g42g42g16g22g255g727g3g3 g21g12g3 g53g20g25g53g21g726g24g254g16g3g55g42g36g38g42g42g42g38g42g42g55g42g55g42g55g36g38g36g36g36g38g38g38g38g42g16g22g255g452g3 g23g17g20g19g3g7735g7783g726g19555g5719g4649g10135g1130g5154g11934g4554g5967g7683g11080g15175g11437g2598g1

21、1253g7997g2511g1411g11444g7783g2801g3626g3344g13556g39g49g36g727g19556g5719g4649g10135g1130g11934g4554g7514g5967g7683g11080g15175 g11437g2598g11253g7997g2511g1411g11444g7783g2801g3626g3344g13556g39g49g36g452g3 g24g3 g1137g16311g1312g3230g1092g16884g3901g3 g24g17g20g3 g51g38g53g6297g3790g1306g452g3 g

22、24g17g21g3 g2061g14118g6208g1791g13099g13583g452g3 g24g17g22g3 g7784g18344g15611g11437g2102g7616g1306g6214g13147g3910g2102g1913g1913g5334g16849g452g3 g24g17g23g3 g2592g5439g20744g17999g2023g2027g12267g5619g7530g726g12267g5619g2251g20g21 g3 g19g19g19 g3 g74g1301g1082g452g3 g24g17g24g3 g5762g9305g8804

23、g9124g19253g452g3 g24g17g25g3 g2016g12769g726g21g3g263g794g23 g3 g263g451g16g21g19 g3 g263g451g16g27g19 g3 g263g452g3 g24g17g26g3 g20744g2491g9885g14844g19253g452g3 g24g17g27g3 g21827g20222g5282g10261g12769g452g3 g24g17g28g3 g11109g4480g3929g5283g726g5967g18431g19g17g19g19g20 g3 g74g452g3 g24g17g20g

24、19g3g5598g18431g2256g7783g3224g726g19g17g20 g3 g173g47g794g21g17g24 g3 g173g47g451g19g17g24 g3 g173g47g794g20g19 g3 g173g47g451g21 g3 g173g47g794g21g19 g3 g173g47g451g20g19 g3 g173g47g794g20g19g19 g3 g173g47g451g21g19 g3 g173g47g794 g21g19g19 g3 g173g47g451g24g19g3g173g47g794g20 g3 g19g19g19 g3 g173

25、g47g452g3 g25g3 g7789g2807g11450g18429g19708g573g1555g4494g2560g17926g17865g3 g25g17g20g3 g7789g2807g18429g19708g3 g6457g42g37g18g55g3g21g27g19g25g26g11444g16305g8818g6295g15996g452g1958g1117g712g2598g10359g7783g2801g2748g15175g14654g7783g2801g2546g10135g49g60g18g55g3g21g26g23g22g452g18423g19702g20g

26、17g19 g3 g74g794g21g17g19 g3 g74g2598g10359g6214g15175 g14654g7022g1214g7783g2801g16059g1117g712g3895g11096g452g3 g25g17g21g3 g7789g2807g1555g4494g2754g17926g17865g3 g18423g19702g11444g7783g2801g7022g13726g1214g21 g3 g263g794g27 g3 g263g7569g1318g1083g1549g4488g7206g19492g304g23g27 g3 g75g727g19375g

27、7503g1549g4488g712g19760g7022g13726g3416g16g27g19 g3 g263g7569g1318g1083g712g1085g2591g2557g3901 g2027g15805g452g19375g17988g17920g17859g7206g712g2591g18423g11096g1549g9305g12769g1117g2256g5282g2016g6214g9186g8798g4598g4657g2622g17920g17969g452g3 g26g3 g7926g9089g7151g8971g3 g26g17g20g3 g7789g2807g3

28、9g49g36g6662g2572g3 g2566g7783g2801g19g17g20 g3 g74g712g11096g9186g8798g2023g2027g11844g12056g14371g12985g7515g10470g712g6457g6256g17977g6425g11444g7997g10393g3626g3344g13556g39g49g36g6656g2566g16901g2162g11522g6616g14720g11444g6909g1420g7145g8965g17931 g15996g6656g2566g7783g2801g39g49g36g452g3 g26g

29、17g21g3 g7789g2807g39g49g36g17246g18437g7926g9089g3 g7783g2801g39g49g36g6656g2566g2622g712g11096g15611g11437g18g7784g18344g2102g7616g1306g18596g4554g7783g2801g39g49g36g17240g18431g712g5507g36g21g25g19g18g36g21g27g19g8708g1644g1130g20g17g27g794g21g17g19g7206g712g17970g2616g1214g51g38g53 g6297g3790g45

30、2g3 g26g17g22g3 g5144g5445g51g38g53g7926g9089g3 g26g17g22g17g20g3 g12642g1078g17828g51g38g53g6303g3796g3 g39g37g23g24g18g55g3g21g19g22g20g178g21g19g20g28g3 g22g3 g6457g1083g2119g7145g8965g18301g2150g21g19 g3 g541g47g3g51g38g53g3g6297g3790g2557g5316g1411g13099g726g21g238g40g68g86g92g3g55g68g84g3g51g3

31、8g53g3g2557g5316g20148g9255g9186g27g17g19 g3 g541g47g712g1082g9320g3910g5445g10393g51g20g708g20g19 g3 g541g80g82g79g18g47g709g19g17g22 g3 g541g47g712g1083g9320g3910g5445g10393g51g26g708g20g19 g3 g541g80g82g79g18g47g709g19g17g22g3g541g47g712g39g49g36g3g8273g7599g11g21g24 g3 g81g74g18g541g47g12g22g17g

32、19 g3 g541g47g712g51g38g53g8804g27g17g23 g3 g541g47g452g3 g12636g1072g17822g51g38g53g6297g3790g2546g7072g726g28g24 g3 g263g20148g2568g5719g24 g3 g80g76g81g712g28g23 g3 g263g2568g5719g20 g3 g80g76g81g712g24g24 g3 g263g17968g9883g20 g3 g80g76g81g712g26g21 g3 g263g5414g1384g20 g3 g80g76g81g712g21g24g11

33、14 g5594g10719g727g26g21 g3 g263g5414g1384g20g19g3g80g76g81g452g12458g11437g4649g10135g11096g7184g14844g1072g13527g8804g1299g7471g7783g2801g39g49g36g8273g7599g452g3 g26g17g22g17g21g3 g12642g1218g17828g51g38g53g6303g3796g3 g6457g1083g2119g7145g8965g18301g2150g21g19 g3 g541g47g3g51g38g53g3g6297g3790g2

34、557g5316g1411g13099g726g21g238g40g68g86g92g3g55g68g84g3g51g38g53g3g2557g5316g20148g9255g9186g27g17g19 g3 g541g47g712g1082g9320g1973g5445g10393g53g20g25g41g21g81 g708g20g19 g3 g541g80g82g79g18g47g709g19g17g22 g3 g541g47g712g1083g9320g1973g5445g10393g53g20g25g53g21g708g20g19 g3 g541g80g82g79g18g47g709

35、g19g17g22 g3 g541g47g712g12636g1072g17822g51g38g53g1239g10393g708g12336g18426g22g19g1597g709g2566g22g17g19 g3 g541g47 g1420g1130g8273g7599g39g49g36g712g51g38g53g3g11096g8804g27g17g23 g3 g541g47g452g3 g12636g1212g17822g51g38g53g6297g3790g2546g7072g726g3g28g24 g3 g263g20148g2568g5719g24 g3 g80g76g81g7

36、12g28g23 g3 g263g2568g5719g20g3g80g76g81g712g25g21 g3 g263g17968g9883g20 g3 g80g76g81g712g26g21 g3 g263g5414g1384g20 g3 g80g76g81g712g22g19 g1114g5594g10719g727g7472g2622g26g21 g3 g263g5414g1384g20g19 g3 g80g76g81g452g12458g11437g4649g10135g1130g7184g14844g1072g13527g8804g452g3 g26g17g23g3 g6303g379

37、6g1245g10399g11450g11115g9001g7926g9089g3 g26g17g23g17g20g3 g12320g2566g17970g18431g10860g14130g13062g2256g1941g20g238g55g36g40g13635g2018g9186g1117g712g2256g10013g14371g4540g1944g9446g16403g712g18301g2150g6208g20g17g24g705g11444g10860g14130g13062g9446g9186g727g12349g17970g2023 g2468g2622g1602g7599g

38、712g4564g9305g1083g2061g3370g6208g10860g14130g13062g2061g14118g452g3 g26g17g23g17g21g3 g2102g2139g2566g21 g3 g541g47g3g25g238g1082g7783g13635g2018g9186g2748g24 g3 g541g47g11444g12636g1212g17822g51g38g53g6297g3790g1239g10393g2144g7136g11444g51g38g53g12753g712g6495g14737g9255g2352g712g21g3g19g19g19 g3

39、 g74g12267 g5619g24g3g86g452g3 g26g17g23g17g22g3 g2102g2139g4662g24g3g541g47g3g39g49g36g2102g4480g18431g7735g2038g10393g2748g12636g1212g17822g51g38g53g1239g10393g9255g2616g9186g1485g8529g2256g1941g10860g14130g13062g2061g14118g11444g9961g7783g4484g2622g17931g15996g11109 g8995g712g11109g8995g1455g1109

40、6g11444g11109g2491g1130g27g19 g3 g57g712g11109g8995g7206g19492g1130g22g19 g3 g80g76g81g452g3 g26g17g24g3 g2067g14124g6214g1797g2108g7622g3 g4662g10860g14130g13062g2061g14118g13726g1214g2061g14118g6208g1791g13099g13583g6208g1791g1306g1082g712g7785g6558g39g49g36g2102g4480g18431g7735g16864g2132g7133g62

41、97g3790g2090g11444g11550g11444g7569g5206g3927g4671g712g4662g11109 g8995g13571g7628g11096g6397g10135g13099g13583g6397g10135g5522g6208g3374g10359g7095g1318g4488g7827g452g3 g27g3 g13577g7634g6661g17958g2560g2138g4560g3 g27g17g20g3 g17246g6621g7741g2044g3 g19555g5719g4649g10135g2090g10720g1072g7569g3927

42、g4671g1130g20 g3 g21g23g19 g3 g69g83g102g24 g3 g69g83g7569g5206g712g19556g5719g4649g10135g2748g12458g11437g4649g10135g7184g7569g5206g7206g2132g4554g1130g4558g20668g7481g7032g452g3 g27g17g21g3 g13577g7634g2138g4560g3 g27g17g21g17g20g3 g19555g5719g726g16091g7920g7783g2801g8995g18051g2090g10720g1072g75

43、69g3927g4671g1130g20 g3 g21g23g19 g3 g69g83g102g24 g3 g69g83g7569g5206g7206g712g2132g1130g19555g5719g712g5621g16305g7206g17931g15996g9083g5311g452g3 g27g17g21g17g21g3 g19556g5719g726g16091g7920g7783g2801g8913g7481g2090g10720g1072g7569g3927g4671g1130g20 g3 g21g23g19 g3 g69g83g102g24 g3 g69g83g7569g52

44、06g712g13567g18429g3901g21g8529g7920g9083g2622g1277g7514g2090g10720g6297g3790g7569 g5206g7206g712g2132g1130g19556g5719g452g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g3 g726g1255g800g3486g1056g1430g1677g2296g5940g1696g2996g3221g1101g1 g4210g5628g4358g1344g4288g3313g1299g883g2232g2351g49g36g51g1g3292g3645g1 g2686g3126g5946g4653g1 g37g35g21g22g16g53g1g19g17g20g18g803g19g17g18g26g1 g2296g5940g1828g3007g5292g3572g1244g2249g1699g4386g4437g4781g4144g2124g4945g694g1278g1156g1 g10366g7545g1097g7487g3g3g1515g7545g5627g12460g3