BS EN ISO 11213-1995 Modified starch - Determination of acetyl content - Enzymatic method《淀粉和淀粉制品的检验 用酶催化法测定淀粉的乙酰含量》.pdf
《BS EN ISO 11213-1995 Modified starch - Determination of acetyl content - Enzymatic method《淀粉和淀粉制品的检验 用酶催化法测定淀粉的乙酰含量》.pdf》由会员分享,可在线阅读,更多相关《BS EN ISO 11213-1995 Modified starch - Determination of acetyl content - Enzymatic method《淀粉和淀粉制品的检验 用酶催化法测定淀粉的乙酰含量》.pdf(14页珍藏版)》请在麦多课文档分享上搜索。
1、BRITISH STANDARD BS EN ISO 11213:1995 Modified starch Determination of acetylcontent Enzymatic method The European Standard EN11213:1995 has the status of a BritishStandardBSENISO11213:1995 This BritishStandard was published under the authority ofthe Standards Board andcomesinto effect on 15June1995
2、 BSI 01-2000 The following BSI references relate to the work on this standard: Committee reference AW/100 Draft for comment 93/507580 DC ISBN 0 580 24179 3 National foreword This BritishStandard has been prepared under the direction of the BSI Standards Board and is the English language version of E
3、N11213:1995 Modified starch Determination of acetyl content Enzymatic method, published by the European Committee for Standardization (CEN). It is identical with ISO11213:1995, published by the International Organization for Standardization (ISO), which has the same title. This BritishStandard has b
4、een produced to fulfil BSIs obligation to publish all approved European Standards but, because of the absence of interest in the UK in the subject concerned, there has been no UK participation in the preparation of EN ISO11213. Any queries relating to the EN should be directed to BSI quoting the ref
5、erence AW/100. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This docu
6、ment comprises a front cover, an inside front cover, pagesi andii, theEN ISO title page, pages2 to8, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front
7、cover. Cross-references Publication referred to Corresponding BritishStandard EN ISO 1666:1973 BS EN ISO1666:1994 Starch Determination of moisture Oven-drying method ISO 3696:1987 BS3978:1987 Specification for water for laboratory use Amendments issued since publication Amd. No. Date CommentsBSENISO
8、11213:1995 BSI 01-2000 i Contents Page National foreword Inside front cover Foreword 2 1 Scope 3 2 Normative references 3 3 Principle 3 4 Reagents and materials 3 5 Apparatus 4 6 Preparation of the sample 4 7 Procedure 4 8 Expression of results 5 9 Precision 6 10 Test report 6 Annex A (informative)
9、Derivation of equation for calculation of acetyl content 7 Annex B (informative) Statistical results of the interlaboratory test 8 Annex C (informative) Bibliography 8 Annex ZA (normative) Normative references to international publicationswiththeir relevant European publications 8 Table 1 Analytical
10、 arrangement for enzymatic determination of acetic acid 6 List of references Inside back coverii blankEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11213 January 1995 ICS 67.180.20 Descriptors: Carbohydrates, starches, chemical analysis, determination of contents, acetyl group, enzymatic
11、method English version Modified starch Determination of acetyl content Enzymatic method (ISO 11213:1995) Amidons modifi Dosage de Lactyle Mthode enzymatique (ISO 11213:1995) Modifizierte Strke Bestimmung des Acetylgehaltes Enzymatisches Verfahren (ISO 11213:1995) This European Standard was approved
12、by CEN on1994-11-28. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards
13、 may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the
14、Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. CEN Europ
15、ean Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1995 Copyright reserved to CEN members Ref. No. EN ISO 11213:1995 EENISO11213:1995 BSI 01-2000 2 Foreword The text of the International Standard IS
16、O11213:1995 has been prepared by Technical Committee ISO/TC93, Starch (including derivatives and by-products), in collaboration withCEN/CS. It has been submitted to Parallel Vote and has been approved on1994-11-28 as a European Standard. This European Standard shall be given the status ofa national
17、standard, either by publication of an identical text or by endorsement, at the latest by July1995, and conflicting national standards shallbe withdrawn at the latest by July1995. According to the CEN/CENELEC Internal Regulations, the following countries are bound to implement this European Standard:
18、 Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland, United Kingdom.ENISO11213:1995 BSI 01-2000 3 1 Scope This International Standard specifies an enzymatic method for the determination of the ac
19、etyl content of modified starch, both granular and soluble in cold water. Total and free acetyl contents are determined and the bound acetyl content is calculated. The method is suitable for determining acetyl contents up to2%(m/m). 2 Normative references The following standards contain provisions w
20、hich, through reference in this text, constitute provisions of this International Standard. At the time of publication, the editions indicated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibili
21、ty of applying the most recent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid International Standards. ISO 1666:1973, Starch Determination of moisture content Oven-drying methods. ISO 3696:1987, Water for analytical laboratory use Specificatio
22、n and test methods. 3 Principle The total acetyl content is determined by heating the sample with dilute hydrochloric acid which hydrolyses the acetyl fraction and solubilizes the starch. In the presence of the enzyme acetyl-CoA synthetase (ACS), acetate is converted with adenosine-5-triphosphate (A
23、TP) and coenzyme A (CoA) to acetyl-Co-A. The latter then reacts with oxaloacetate to form citrate in the presence of citrate synthase (CS). The oxaloacetate required for the reaction is formed from malate and nicotinamide adenine dinucleotide (NAD) in the presence of malate-dehydrogenase (MDH). In t
24、his reaction, the NAD is reduced to NADH and the formation of NADH can be determined by measuring the increase in absorbance at a specified wavelength. (Seereference1 in Annex C.) The free acetyl content is determined by making a suspension of the modified starch in water, filtering, and determining
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