DB45 T 2138-2020 水体中诺如病毒的核酸检测方法 实时荧光RT -PCR法.pdf

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1、g44g38g54g3 g20g20g17g19g21g19g3 g38g3g24g20g3 g3g3g3g3g3g3 DB45 g5301g16309g3876g7173g14368g8945g2416g3430g7151g7741g2044g3 g39g37g23g24g18g55g3g21g20g22g27g258g21g19g21g19g3 g3g3g3g3g3g3 g8810g1417g1123g16944g4024g11259g8712g11450g7790g18350g7926g9089g7151g8971g3g3g3 g4564g7212g14749g1919g53g55g16

2、g51g38g53g8971g3 g39g72g87g72g70g87g76g82g81g3g82g73g3g81g82g85g82g89g76g85g88g86g3g81g88g70g79g72g82g87g76g71g72g3g68g70g76g71g3g76g81g3g90g68g87g72g85g178g3g53g72g68g79g16g87g76g80g72g3g53g55g16g51g38g53g3 g3 g3 g3 g21g19g21g19g3g16g3g19g26g3g16g3g21g23g2567g5177g3 g21g19g21g19g3g16g3g19g27g3g16g3

3、g21g19g4564g7155 g5301g16309g3876g7173g14368g8945g2416g5176g3440g11527g11673g12759g10812g4726g3 g2567g5177 g3 g39g37g23g24g18g55g3g21g20g22g27g178g21g19g21g19g3 g265g3 g2179g3g3g16438g3 g7516g7735g2038g6457g10135g42g37g18g55g3g20g17g20g252g21g19g19g28g13577g2090g11444g16372g2121g17319g14713g452g3 g7

4、516g7735g2038g11105g5295g16303g3870g7167g14362g8939g2410g2459g11087g1685g5351g4100g2696g1354g6656g2090g5286g4563g17247g452g3 g7516g7735g2038g11105g5295g16303g3870g7167g14362g8939g2410g2459g11087g6320g7519g7735g2038g4100g2696g1354g5506g2579g452g3 g7516g7735g2038g17319g14713g2437g1405g726g5295g16303g3

5、870g7167g14362g8939g2410g11246g11253g20148g19554g6615g2150g1117g5619g452g3 g7516g7735g2038g1131g16305g17319g14713g1258g726g2120g5117g451g18115g1133g1133g451g16989g2012g7861g451g20636g4535g10245g451g19151g19865g451g7550g12272g7794g452g3 g20g3 g8810g1417g1123g16944g4024g11259g8712g11450g7790g18350g792

6、6g9089g7151g8971g3g3g4564g7212g14749g1919g53g55g16g51g38g53g8971g3 g20g3 g14649g3370g3 g7516g7735g2038g16372g4554g1206g8804g1411g1117g16938g4018g11253g8706g11444g4558g7206g14743g1913g53g55g16g51g38g53g7920g9083g7145g8965g452g3 g7516g7735g2038g17970g11096g1214g5295g16303g2410g3599g1973g3424g16024g880

7、4g451g3424g1083g8804g451g19702g1117g5439g1483g8804g451g4193g1152g11096g8804g451g8849g8804g12665g8804g1411g1117g3626g3344g265g13556g2748g3626g3344 g266g13556g16938g4018g11253g8706g11444g7920g9083g452g3 g21g3 g7525g16931g2754g4560g1151g3 g1083g2119g7519g16925g2748g4554g1145g17970g11096g1214g7516g7095g

8、1318g452g3 g21g17g20g3 g3 g16944g4024g11259g8712g3g3g49g82g85g82g89g76g85g88g86g3 g1094g11132g14643g3364g1973g5445g17319g5717g5719g14067g14032g9918g7396g2561g2748g7059g2561g11444g18429g16305g11253g2511g1411g452g3927g3914g7072g16938g4018g11253g8706g5445g17319g11444g8804g9408g5719g7396g2561g11227g5877

9、g7263g11105 g1214g20382g11096g8804g6214g11087g9067g11096g8804g16091g8849g7683g6256g14372g452g16938g4018g11253g8706g4750g1214g1258g12971g7583g10470g11253g8706g12289g11444g16938g4018g11253g8706g4750g712g7263g1072g13556g5522g5681g11560g1388g451g6343g2511 g5719g11157g7481g1085g2620g11444g11253g8706g2016

10、7g12994g452g16938g4018g11253g8706g11556g5556g21g25 g3 g81g80g794g22g24 g3 g81g80g712g7184g2357g14284g712g16024g19858g12999g13065g712g10803g5522g712g2680g1212g2417g19858g1411g4649g12320g452g16938 g4018g11253g8706g3626g3344g13556g7263g2437g14033g8595g19246g53g49g36g712g1944g19375g26g17g24 g3 g78g69g79

11、4g26g17g26 g3 g78g69g712g7785g6558g3626g3344g10409g5553g712g16938g4018g11253g8706g16091g2102g1130g25g1114g3626g3344g13556 g708g74g72g81g82g74g85g82g88g83g712g739g265g794g739g268g709g712g739g265g2748g739g266g7263g5445g17319g1258g12971g5717g5719g14067g14032g9918g11444g1108g1114g1131g16305g3626g3344g13

12、556g712g739g268g1167g2591g5967g7683g1258g712 g1398g5560g4673g16091g7920g2090g452g3 g21g17g21g3 g3 g4564g7212g14749g1919g17980g17826g5515g16g13968g2622g18348g19252g5445g2563g5322g3g3g53g72g68g79g16g87g76g80g72g3g53g55g16g51g38g53g3 g3416g53g55g16g51g38g53g2557g5316g1411g13099g1117g2256g1941g14743g191

13、3g3626g3346g712g2137g11096g14743g1913g1553g2599g13151g12319g4558g7206g11521g9083g7076g1114g51g38g53g17931g12347g712g7472g2622g17994g17911g38g87g1644g4649g8273 g7599g17931g15996g4554g5719g452g3 g21g17g22g3 g3 g38g87g1650g3g3g38g92g70g79g72g3g87g75g85g72g86g75g82g79g71g3g89g68g79g88g72g3 g8703g1114g51

14、g38g53g2557g5316g12753g1973g14743g1913g1553g2599g2144g17902g16878g4554g11444g3599g1644g7206g6256g13567g2486g11444g5594g10719g7072g452g2612g8273g7599g11444g38g87g1644g1086g16917g8273g7599g11444g17319g4091g6439g17229 g7072g11444g4649g7072g4488g3416g13551g5719g1955g13099g712g17319g4091g6439g17229g7072g

15、17338g3914g712g38g87g1644g17338g4671g452g2557g1147g1238g10086g452g3 g22g3 g2517g10812g3 g8804g7783g3416g17994g17911g5206g17231g12267g4480g9255g2616g13524g13604g13136g14284g7206g712g5206g8595g12267g4480g11444g11253g8706g16091g2664g19572g2144g14284g1082g712g10086g2622g1981g9031g14177g11253g8706g712g52

16、86g11096g13962g1161 g1212g18359g8889g9200g11253g8706g17902g2144g4604g19702g9091g13657g11444g7032g7628g452g5316g11096g4558g7206g14743g1913g53g55g16g51g38g53g6320g7519g712g19128g4649g16938g4018g11253g8706g16019g3875g15611g11437g2410g49g18g54g2410g16878g16849g10409 g5426g5719g5445g10393g2748g6610g19128

17、g712g14743g1913g6610g19128g11444g24g255g12575g7735g16864g6357g2682g14743g1913g3626g3346g712g22g255g12575g7735g16864g9244g9885g14743g1913g3626g3346g712g5507g6610g19128g4540g7076g7206g712g6357g2682 g14743g1913g3626g3346g11444g2561g4660g8978g19375g16091g9244g9885g14743g1913g3626g3346g11444g9712g2561g89

18、78g19375g2664g7014g727g5507g51g38g53g6297g3790g7206g712g55g68g84g18342g11444g24g255g279g22g255g12575g3910g2103g18342g9067g5719g4662 g6610g19128g18342g2103g8804g16403g712g6357g2682g14743g1913g1085g1981g2567g9244g9885g14743g1913g11444g5537g2813g712g1455g14743g1913g11521g9083g13099g13583g7014g19702g214

19、4g6357g2682g3626g3346g11444g14743g1913g1553g2599g712g2467g8703g6297 g3790g1072g7569g39g49g36g19246g712g2591g1239g11087g1072g1114g6357g2682g3626g3346g11444g14743g1913g1553g2599g712g4558g10720g14743g1913g1553g2599g12319g13151g1086g51g38g53g1239g10393g5522g6208g2620g8597g452g3 g23g3 g16907g2168g2754g13

20、901g7558g3 g23g17g20g3 g16907g2168g3 g23g17g20g17g20g3 g19604g2582g7481g16932g7230g3910g712g7516g7095g1318g6256g1455g11096g11444g16901g2162g3447g1130g2102g7616g13535g712g2102g4480g11087g10393g4502g16901g2162g6256g11096g8804g1130g7184g53g49g36g18342g11444g8804g452g3 g39g37g23g24g18g55g3g21g19g22g27g1

21、78g21g19g21g19g3 g21g3 g23g17g20g17g21g3 g21g17g24 g3 g80g82g79g18g47g8799g2374g19313g708g48g74g38g79g21g709g9446g9186g712g18301g2150g18085g10135g19572g5509g36g1117g11444g36g17g20g6295g15996g452g3 g23g17g20g17g22g3 g22g705g10379g14009g9128g14271g9031g14177g9186g712g18301g2150g18085g10135g19572g5509g

22、36g1117g11444g36g17g21g6295g15996g452g3 g23g17g20g17g23g3 g20g25g705g51g40g42g27g19g19g19g9446g9186g712g18301g2150g18085g10135g19572g5509g36g1117g11444g36g17g22g6295g15996g452g3 g23g17g20g17g24g3 g19g17g21 g3 g80g82g79g18g47g11444g49g68g21g43g51g50g23g9446g9186g712g18301g2150g18085g10135g19572g5509g

23、36g1117g11444g36g17g23g6295g15996g452g3 g23g17g20g17g25g3 g20 g3 g80g82g79g18g47g11520g18344g9446g9186g451g20 g3 g80g82g79g18g47g8786g8791g2374g19152g9446g9186g451g53g49g36g6656g2566g16901g2162g11522g451g4558g7206g14743g1913g53g55g16g51g38g53g16901g2162g11522g452g3 g23g17g20g17g26g3 g5445g10393g2748

24、g6610g19128g452g5141g1420g9091g5334g3447g1130g20g19 g3 g173g80g82g79g18g47g712g3g16g21g19 g3 g263g1549g4488g712g5445g10393g2748g6610g19128g5311g2119g16369g16024g20g452g3 g16030g20g3 g4564g7212g14749g1919g5451g10399g2754g6616g19134g5317g2125g16030g3 g3626g3344g13556g3 g5445g10393g18g6610g19128g3 g531

25、1g2119g3 g42g265g3 g45g45g57g20g41g3g11g1082g9320g5445g10393g12g3 g24g10g16g42g38g38g36g55g42g55g55g38g38g42g44g55g42g42g36g55g42g16g22g10g3 g45g45g57g20g53g3g11g1083g9320g5445g10393g12g3 g24g10g16g55g38g38g55g55g36g42g36g38g42g38g38g36g55g38g36g55g38g36g55g16g22g10g3 g45g45g57g20g51g3g11g6610g19128

26、g12g3 g24g10g16g41g36g48g16g55g42g55g42g42g36g38g36g42g42g36g42g36g55g38g42g38g36g36g55g38g55g38g16g37g43g52g16g22g10g3 g42g266g3 g45g45g57g21g41g3g11g1082g9320g5445g10393g12g3 g24g10g16g38g36g36g42g36g42g55g38g36g36g55g42g55g55g55g36g42g42g55g42g42g36g55g42g36g42g16g22g10g3 g38g50g42g21g53g3g11g108

27、3g9320g5445g10393g12g3 g24g10g16g55g38g42g36g38g42g38g38g36g55g38g55g55g38g36g55g55g38g36g38g36g16g22g10g3 g53g44g49g42g21g3g11g6610g19128g12g3 g24g10g16g41g36g48g16g55g42g42g42g36g42g42g42g38g42g36g55g38g42g38g36g36g55g38g55g16g37g43g52g16g22g10g3 g23g17g21g3 g13901g7558g3 g2357g6428g1301g1083g1039

28、3g2801g726g3 g252g252g9255g2616g11917g18344g13524g13604g13136g14284g712g4484g5556g19g17g23g24 g3 g173g80g727g3 g252g252g5659g17999g4554g5719g9492g13544g727g3 g252g252g12267g5619g12753g712g20g17g24 g3 g80g47g451g24g19 g3 g80g47g727g3 g252g252g19g17g21 g3 g80g47g17983g7230g15284g3825g51g38g53g12753g45

29、2g3 g24g3 g1312g3230g16884g3901g3 g2357g6428g1301g1083g10393g2801g726g3 g252g252g11109g4480g3929g5283g712g13038g5334g305g19g17g19g20 g3 g74g727g3 g252g252g20744g17999g2592g5439g2023g2027g12267g5619g7530g712g2591g6615g9305g14371g23 g3 g263g712g12267g5619g17999g5334g305g20g22 g3 g19g19g19 g3 g74g727g3

30、 g252g252g8804g17911g9492g16117g13726g727g3 g252g252g8595g2491g2251g8997g727g3 g252g252g6495g14737g9255g2352g3224g727g3 g252g252g20744g2491g9885g14844g3224g727g3 g252g252g4558g7206g14743g1913g51g38g53g6297g3790g1306g727g3 g252g252g1212g13527g11087g10393g4537g1944g7692g452g3 g25g3 g7926g9089g9079g123

31、53g3 g16369g3374g20g452g3 g39g37g23g24g18g55g3g21g20g22g27g178g21g19g21g19g3 g22g3 g3 g3380g20g3 g8810g1417g1123g16944g4024g11259g8712g11450g7790g18350g7926g9089g9079g12353g3 g26g3 g8810g7789g2179g3898g10812g3 g26g17g20g3 g8810g7789g17926g17975g1092g1555g4494g3 g17920g17859g17911g12347g1117g1549g644

32、9g8804g7783g9305g5334g3416g19 g3 g263g794g24 g3 g263g452g4558g20668g4564g6613g2144g8804g7783g2622g5316g23g27 g3 g75g1973g17931g15996g7920g9083g452g4018g7514g2554g7206g7920g9083g712g5316 g4662g8804g7783g13726g1214g16g27g19 g3 g263g2016g7692g1549g4488g5557g7920g452g3 g26g17g21g3 g11259g8712g4610g19708

33、g9097g13663g3 g26g17g21g17g20g3 g8804g17911g9492g16117g13726g451g2202g2096g451g19322g4480g12665g20744g2491g9885g14844g3895g11096g452g3 g26g17g21g17g21g3 g20g3g47g8804g7783g1117g6634g1941g20g19 g3 g173g47g2934g2801g2374g11444g17911g12347g6615g2150g11253g8706g712g2256g1941g21g19 g3 g80g47g11444g21g17g

34、24 g3 g80g82g79g18g47g8799g2374g19313g9446g9186g712g1981g11096g20 g3 g80g82g79g18g47 g11520g18344g16947g14514g8804g7783g83g43g14371g22g17g19g102g19g17g24g452g4018g1130g9255g9082g5334g17843g3927g6214g7538g17240g17843g3914g11444g8804g7783g15g19760g20148g1912g4662g8804g7783g1214g23 g3 g263g451g22g3g24g

35、19g19 g3 g74g12267g5619 g22g19 g3 g80g76g81g712g2566g1082g9269g452g3 g26g17g21g17g22g3 g13556g16117g8804g17911g9492g16117g13726g712g5286g1086g8595g2491g2251g8997g17934g6613g452g2655g2264g8595g2491g2251g8997g712g1455g8804g7783g13635g6034g17994g17911g4484g5556g1130g19g17g23g24 g3 g173g80g11444g9255g26

36、16 g11917g18344g13524g13604g13136g14284g712g4018g1130g9082g5334g17843g3927g6214g7538g17240g17843g3914g11444g8804g7783g712g2591g3790g2256g1072g4722g5659g17999g4554g5719g9492g13544g1214g9255g2616g11917g18344g13524g13604g13136g14284g1082g712g10086 g2622g11096g19322g4480g4662g11917g18344g13524g13604g131

37、36g14284g2566g2090g712g2202g11966g6208g17897g19375g19g17g24 g3 g70g80g11444g4671g7145g3463g712g7022g1941g20148g1912g16117g7481g20g19 g3 g80g47g11444g22g705g10379g14009g14271g9031g14177g9186 g2554g21g20167g4671g10731g10931g10768g11444g24g19 g3 g80g47g12267g5619g12753g1117g452g3 g26g17g21g17g23g3 g110

38、96g6495g14737g9255g2352g3224g6495g14737g21g19 g3 g80g76g81g794g22g19 g3 g80g76g81g712g2566g1082g9269g9186g17820g12331g14371g5282g2032g11444g24g19 g3 g80g47g12267g5619g12753g1117g452g3 g26g17g21g17g24g3 g2256g1941g12665g1411g12319g11444g20g25g705g3g51g40g42g27g19g19g19g9446g9186g712g6495g14737g1909g2

39、102g9255g2352g712g16947g14514g83g43g1644g14371g26g17g19g712g23 g3 g263g3g19849g13726g21 g3 g75g794g22 g3 g75g452g3 g26g17g21g17g25g3 g23 g3 g263g451g20g19 g3 g19g19g19 g3 g74g12267g5619g24 g3 g80g76g81g712g5427g1082g9269g2622g712g4662g24g19 g3 g80g47g12267g5619g12753g1117g11444g9446g9186g1944g18200g

40、1646g1602g3416g5282g2032g11444g9492g13544g1082g452g3 g26g17g21g17g26g3 g2256g20g19g19 g3 g173g47g794g21g19g19 g3 g173g47g11444g19g17g21 g3 g80g82g79g18g47g3g49g68g21g43g51g50g23g712g6495g14737g712g11556g14371g4540g1944g18429g5852g8889g9200g452g3 g26g17g21g17g27g3 g23 g3 g263g451g20g19 g3 g19g19g19 g

41、3 g74g12267g5619g24 g3 g80g76g81g712g2566g1082g9269g2467g1130g11253g8706g9091g13657g9186g452g3 g2202g11966g9492g14284g14g10379g14009g9128g14271g9031g14177g9186g3 g1082g9269g9186g14g51g40g42g27g19g19g19g9446g9186g3 g8889g9200g14g21g19g19 g3 g173g47g3g49g68g21g43g51g50g23g3 g11253g8706g9091g13657g9186

42、g3 g11253g8706g53g49g36g6656g2566g3 g4558g7206g14743g1913g53g55g16g51g38g53g3 g17911g9492g3 g6495g14737g9031g14177g21g19g794g22g19 g3 g80g76g81g3 g23 g3 g263g19849g13726g21g794g22 g3 g75g3 g23 g3 g263g712g20g19g3g19g19g19 g3 g74g3g12267g5619g24 g3 g80g76g81g3 g2199g9976g6495g14737g24 g3 g80g76g81g14

43、371g18429g5852g8889g9200g3 g23 g3 g263g712g20g19g3g19g19g19 g3 g74g3g12267g5619g24 g3 g80g76g81g712g2566g1082g9269g3 g20 g3 g47g8804g7783g14g17911g12347g6615g2150g11253g8706g14g8799g2374g19313g9446g9186g3 g39g37g23g24g18g55g3g21g19g22g27g178g21g19g21g19g3 g23g3 g27g3 g4564g7212g14749g1919g53g55g16g5

44、1g38g53g7926g9089g3 g27g17g20g3 g11259g8712g53g49g36g6662g2572g3 g27g17g20g17g20g3 g1455g11096g11253g8706g53g49g36g6656g2566g16901g2162g11522g17931g15996g53g49g36g11444g6656g2566g712g2591g7785g6558g1306g3224g2748g16901g2162g11522g16305g8818g16947g7076g6909g1420g8597g20692g452g1301g16938g4018g11253 g

45、8706g19555g5719g11444g13018g1519g5852g9186g7783g7516g6214g2934g2801g2374g11444g16938g4018g11253g8706g7783g7516g1130g7784g18344g6656g2566g17911g12347g19555g5719g4649g10135g712g1301g2556g15080g8804g1420g1130g7784g18344g6656g2566g17911g12347 g19556g5719g4649g10135g452g3 g27g17g20g17g21g3 g4662g11253g87

46、06g9091g13657g9186g2256g1941g20148g1912g16117g7481g24g25g19 g3 g173g47g16114g16403g9186g11444g20g17g24 g3 g80g47g12267g5619g12753g1117g712g9255g2352g712g4564g9305g7022g13726g20g19 g3 g80g76g81g712g1981g2256 g1941g24g25g19 g3 g173g47g7184g8804g1161g18359g712g9255g2352g452g3 g27g17g20g17g22g3 g2566g25

47、g24g19 g3 g173g47g9255g2616g9186g2256g1941g2144g13535g2374g7713g1082g712g27 g3 g19g19g19 g3 g74g12267g5619g20 g3 g80g76g81g5427g7014g19702g12753g1117g11444g12267g5619g9186g452g3 g27g17g20g17g23g3 g4662g20g17g24 g3 g80g47g12267g5619g12753g1117g2201g1417g11444g9255g2616g9186g1944g18200g2256g1941g2144g

48、13535g2374g7713g11444g1082g712g27 g3 g19g19g19 g3 g74g12267g5619g20 g3 g80g76g81g712g5427g12267g5619g9186g452g3 g27g17g20g17g24g3 g1214g13535g2374g7713g1082g2256g1941g24g20g19 g3 g173g47g9031g9186g20g712g27 g3 g19g19g19 g3 g74g12267g5619g20 g3 g80g76g81g712g5427g12267g5619g9186g452g3 g27g17g20g17g25

49、g3 g1214g13535g2374g7713g1082g2256g1941g24g20g19 g3 g173g47g9031g9186g21g712g20g22 g3 g19g19g19 g3 g74g12267g5619g3g20 g3 g80g76g81g712g5427g12267g5619g9186g452g3 g27g17g20g17g26g3 g7460g6546g7136g11444g7014g19702g12753g712g20g22 g3 g19g19g19 g3 g74g12267g5619g20 g3 g80g76g81g452g3 g27g17g20g17g27g3

50、 g4662g13535g2374g7713g7022g13726g2144g5282g2032g11444g20g17g24 g3 g80g47g12267g5619g12753g1082g712g2625g7713g1117g5619g2256g1941g22g24 g3 g173g47g3g53g49g36g9031g14177g9186g712g4564g9305g7022g13726g24 g3 g80g76g81g452g3 g27g17g20g17g28g3 g27 g3 g19g19g19 g3 g74g12267g5619g20 g3 g80g76g81g712g12267g