ISO 13171-2016 Essential oil of oregano [Origanum vulgare L subsp hirtum (Link) letsw]《牛至[Origanum vulgare L subsp hirtum (Link) letsw]精油》.pdf

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1、 ISO 2016 Essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw Huile essentielle dorigan Origanum vulgare L. subsp. hirtum (Link) letsw INTERNATIONAL STANDARD ISO 13171 First edition 2016-08-01 Reference number ISO 13171:2016(E) ISO 13171:2016(E)ii ISO 2016 All rights reserved COP

2、YRIGHT PROTECTED DOCUMENT ISO 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet,

3、without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 copyrightiso.org www

4、.iso.org ISO 13171:2016(E)Foreword iv 1 Scope . 1 2 Normative references 1 3 T erms and definitions . 1 4 Requirements 1 5 Flash point . 2 6 Sampling 2 7 Packaging, labelling, marking and storage . 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential o

5、il of oregano Origanum vulgare L. subsp. hirtum (Link) letsw .4 Annex B (informative) Flashpoint . 7 Bibliography 8 ISO 2016 All rights reserved iii Contents Page ISO 13171:2016(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodie

6、s (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations,

7、 governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further m

8、aintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).

9、Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introdu

10、ction and/or on the ISO list of patent declarations received (see www.iso.org/patents). Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. For an explanation on the meaning of ISO specific terms and expressions related to co

11、nformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword Supplementary information The committee responsible for this document is ISO/TC 54, Essential oils.iv ISO 2016 Al

12、l rights reserved INTERNATIONAL ST ANDARD ISO 13171:2016(E) Essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw 1 Scope This International Standard specifies certain characteristics of the essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw, in order to facili

13、tate the assessment of its quality. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the refe

14、renced document (including any amendments) applies. ISO/TS 210, Essential oils General rules for packaging, conditioning and storage ISO/TS 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative den

15、sity at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 11024 (all parts), Essential oils General guidance on chromatographic profiles 3 Terms a

16、nd definiti ons For the purposes of this document, the following terms and definitions apply. 3.1 essential oil of oregano essential oil obtained by steam distillation of the flowering tops of Origanum vulgare L. subsp. hirtum (Link) letsw, of the Lamiaceae family, growing mainly in Germany, Netherl

17、ands and Hungary Note 1 to entry: For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw shall meet the requirements as given in Table 1. ISO 2016 All rights reserved 1 ISO 13171:2016(E) Table 1 Requirements for

18、 the essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw Characteristics Requirements Test method Appearance Clear, mobile liquid Colour Yellow to dark brownish red Odour Characteristic, aromatic, phenolic, with a slightly spicy base Relative density at 20 C d 0,930 - 0,955 ISO 2

19、79 Refractive index at 20 C 1,500 - 1,513 ISO 280 Optical rotation at 20 C Between 5 and +2 ISO 592 Miscibility in ethanol, 80 % (volume frac- tion), at 20 C It shall not be necessary to use more than 2 vol- umes of ethanol, 80 % (volume fraction), to obtain a clear solution with 1 volume of essenti

20、al oil. Sometimes opalescence can arise on continuing the addition of ethanol. ISO 875 4.2 Carry out the analysis of the essential oil by gas chromatography. Determine the chromatographic profile in accordance with ISO 11024 (all parts). Identify in the chromatogram obtained, the representative and

21、characteristic components shown in Table 2. The proportions of these components, indicated by the integrator, shall be as shown in Table 2. This constitutes the chromatographic profile of the essential oil. T a b l e 2 C h r o m a t o g r a p h i c p r o f i l e Component Minimum % Maximum % -Thujen

22、e 0,2 1,5 -Pinene 0,2 2,5 Myrcene 0,5 3,0 -Terpinene 0,5 2,0 p-Cymene 4,0 10,0 -Terpinene 3,0 9,0 Linalool traces a 3,0 Terpinen-4-ol 0,5 2,0 Thymol 0,5 5,0 Carvacrol 60,0 80,0 -Caryophyllene 0,5 4,0 atraces: 0,01 %. NOTE The chromatographic profile is normative, contrary to typical chromatograms gi

23、ven for information in Annex A. 5 Flash point Information on the flash point is given in Annex B. 6 Sampling Sampling shall be performed in accordance with ISO 212.2 ISO 2016 All rights reserved ISO 13171:2016(E) Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specifi

24、ed in this International Standard to be carried out at least once. 7 Packaging, labelling, marking and storage These items shall be in accordance with ISO/TS 210 and ISO/TS 211. ISO 2016 All rights reserved 3 ISO 13171:2016(E) Annex A (informative) Typical chromatograms of the analysis by gas chroma

25、tography of the essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw4 ISO 2016 All rights reserved ISO 13171:2016(E)P eak identification Operating conditions 1 -Thujene Column: fused capillary silica, 30 m length, 0,25 mm internal diameter Stationary phase: SPB TM1 (SE-30) a Film

26、thickness: 0,25 m Oven temperature: programming temperature from 50 C to 180 C at a rate of 3 C/ min and 180 C to 220 C at a rate of 10 C/min Injector temperature: 260 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: helium Volume injected: 1 l Carrier gas flow rate: 1 ml/m

27、in Split ratio: 1/50 aSPB TM1 (SE-30) is an example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product 2 -Pinene 3 Myrcene 4 -Terpinene 5 p-Cymene 6 -Terpinene 7 Linalool

28、8 Terpinen-4-ol 9 Thymol 10 Carvacrol 11 -Caryophyllene Figure A.1 Typical chromatogram taken on an apolar column ISO 2016 All rights reserved 5 ISO 13171:2016(E)P eak identification Operating conditions 1 -Pinene Column: fused capillary silica, 30 m length, 0,25 mm internal diameter Stationary phas

29、e: Supelco Wax TM10 a Film thickness: 0,25 m Oven temperature: programming temperature from 50 C to 180 C at a rate of 3 C/min and 180 C to 220 C at a rate of 10 C/min Injector temperature: 260 C Detector temperature: 280 C Detector: flame ionization type Carrier gas: helium Volume injected: 1 l Car

30、rier gas flow rate: 1 ml/min Split ratio: 1/50 aSupelco Wax TM10 is an example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product 2 -Thujene 3 Myrcene 4 -Terpinene 5 p-Cym

31、ene 6 Terpinene 7 Linalool 8 -Caryophyllene 9 Terpinen-4-ol 10 Thymol 11 Carvacrol Figure A.2 Typical chromatogram taken on a polar column6 ISO 2016 All rights reserved ISO 13171:2016(E) Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance compa

32、nies, and people in charge of safety services require information on the flash points of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standard

33、ization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high priced essential oils; as there are several different types of equipment which can be used for the determination

34、, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties. The equipment with which this value was obtained shall

35、 be specified. For further information see ISO/TR 11018. B.2 Flashpoint of the essential oil of oregano Origanum vulgare L. subsp. hirtum (Link) letsw The mean value is +65 C. NOTE Obtained with “closed cup” 1)equipment. 1) Equipment available commercially. This information is given for the convenie

36、nce of users of this document and does not constitute an endorsement by ISO of this product. ISO 2016 All rights reserved 7 ISO 13171:2016(E) Bibliography 1 ISO 3218, Essential oils Principles of nomenclature 2 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 3 ISO/TR 21092, Essential oils Characterization8 ISO 2016 All rights reserved ISO 13171:2016(E) ISO 2016 All rights reserved IC S 71.100.60 Price based on 8 pages