GOST EN 15891-2013 Foodstuffs Determination of deoxynivalenol in cereals cereal products and cereal based foods for infants and young children HPLC method with immunoaffinity colum.pdf

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1、 EN 15891 2013III - , - . ( ) - . , , 2014 -, EN 15891 2013IV EN 15891 201324 4.1 N ISO 3696:1995. . , - .4.2 (Na2HPO4 12H2O).4.3 .4.4 .4.5 .4.6 .4.7 , w (HCl) = 37 %.4.8 , (HCl) = 0,1 /3 8,28 3 4.7 1 3.4.9 , c(NaOH) = 0,1 /3 4 4.6 1 3 .4.10 - c(NaCl) = 120 /3, c(Cl) = 2,7 /3, c(Na2HPO4 + H2PO4) = 1

2、0 / 3, 7,4 . pH 8,0 4.5, 1,2 2,9 (Na2HPO4 12H2O) 4.2, 0,2 4.4 0,2 4.3 900 3 . 7,4 . pH - 4.8 4.9, 1 3. .4.11 . , , , - . , , .4.12 8000 /.4.13 .4.14 96 % 100 %.4.15 9,5 4.13 90,5 .4.16 15 4.13 85 . 0,1 4.14. - ( ) . .4.17 50 4.13 50 .4.18 EN 15891 20133 , - , , - 1000 80 % 500 1 2 3.4.19 97 %. . , ,

3、 - .4.20 , 1,25 /3 ( I) 4.19 5 4,0 3 4.11. 1,25 /3. - . I 18 , - 12 . I 6 .4.21 , 250 /3 ( II) I 4.20 800 3 4.11 4 3. 250 /3. II 18 , 12 . II 6 - .4.22 , II 4.21 200 3 4.11 2 3. 25 /3. 200 270 5 5.16. - . , . , DON, /3, max 100DONA Mb = , (1) max ( 220 ); , / ( = 296,3 / );e 4.11, 2/ , ( 681 2/ , 1);b , . II, DON 2, /3, - DON 2 = DON 10 (2) 18 . - 12 . 6 EN 15891 20134 - . , .4.23 , 100 /3 II 4.21, 500 , - 5 3, 4.11. 18 . - 12 . 6 - .4.24 , 10 /3 4.23 500 3 - 5 3, 4.11. 18 . - 12 . 6 - .5 5.1 - , , .5.2 , 0,0001 .5.3 ,