GOST 31878-2012 Animal feeding stuffs Method for the detection and enumeration of coliforms Most probable number technique《动物饲料 大肠杆菌群的检测和枚举方法 最近似数技术》.pdf

《GOST 31878-2012 Animal feeding stuffs Method for the detection and enumeration of coliforms Most probable number technique《动物饲料 大肠杆菌群的检测和枚举方法 最近似数技术》.pdf》由会员分享，可在线阅读，更多相关《GOST 31878-2012 Animal feeding stuffs Method for the detection and enumeration of coliforms Most probable number technique《动物饲料 大肠杆菌群的检测和枚举方法 最近似数技术》.pdf（12页珍藏版）》请在麦多课文档分享上搜索。

1、 ( ). (ISO 4831:2006, NEQ) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)318782012, - 1.092 . 1.22009 . -, . , , 1 - ( )2 3 , ( 3 2012 . 54-) : ( 3166) 00497 ( 3166) 00497 KGRUTJUZUA 4 29 2012 . 1847- 318782012 - 1 2014 .5 ISO 4831:2006 Microbiology offood and animal fe

2、eding stuffs Horizontal method for the detection and enumeration of coliforms Mostprobable number technique ( . . ) (NEQ). 5398520106 ( ) - . , - . - , 2013 -, II 318782012 ( ). Animal feeding stuffs. Method for the detection and enumeration of coliforms.Most probable number technique1 - () (). , 1

3、100 .2 : IS 72182011 . - ISO 11133-12011 . - . 1. - ISO 11133-22011 . - . 2. - 13496.080 , . 2531182 . 2922791 (ISO 835-181) . . 1. 317472012 . ( ) - , 1 , , . (), (-) . , , , , .1 318782012 201401013 31747.4 () 31747.4.1 4.1.1 1 , 30 C 37 C ( ) -2448.4.1.2 4.1.1 / . , / , 1 3 30 C 37 C ( ) -2448.4.

4、1.3 , / - , 4.1.2.4.2 4.2.1 - , , .4.2.2 - , -, ., - .4.2.3 , , 30 C 37 C ( ) 24 , -, , - 24 48 , .4.2.4 - - , .4.2.5 4.2.4 30 C 37 C 24 48 .4.2.6 1 3 1 () - 4.2.5, . - .5 5.1 , ISO 7218, ISO 11133-1 ISO 11133-2.5.2 : LST ( )5.2.1 1.2 318782012 1 ) b) - , 40 20 (C12H22O11c215 H2O), 10 5 (K2HPO4), 5,

5、5 2,75 (KH2PO4), 5,5 2,75 , 10 5 , 0,2 0,1, 31000 10005.2.2 . pH , 6,8 c177 0,2 . pH 25 C. 10 3 () - 16 c180 160 , , - 20 c180 200 ( ), . (121 c177 1) C 15 . .5.2.3 ISO 11133-1. -LST() ISO11133-2(B.1).5.3 : 5.3.1 2. 2 , 10 (C12H22O11c215 H2O), 10 , 20 (), 0,0133, 310005.3.2 . pH , 7,2 c177 0,2 . pH

6、25 C. 5.2.2.5.3.3 ISO 11133-2 ( B.1).6 ISO 7218.6.1 ( ) ().6.2 , (30 c177 1) C (37 c177 1) C.6.3 , - , - 3 , .3 3187820126.4 , 16 c180 160 20 c180 200 .6.5 , , 16 c180 160 (6.4).6.6 1 3103 29227.6.7 pH- 2 18, c177 0,01 . - 25 C.7 , .8 13496.0, 25311 , .9 (. A)9.1 (. , A.1)9.1.1 13496.0, 25311 , , .9

7、.1.2 9.1.2.1 , x 3 , , x 3 , - , 10 3 5.2.1, a), 1 3x103, , 10 3 - 5.2.1, b), x c16313.9.1.2.2 (9.1.2.1) - (6.2), 30 C 37 C ( ), (24 c177 2) .9.1.2.3 (9.1.2.1) - (6.2), 30 C 37 C ( ) (24 c177 2) , , , - , (24 c177 2) .9.1.3 (. , A.3)9.1.3.1 , 9.1.2.2, (6.3) - (5.3). (6.2), - 30 C 37 C ( ) (24 c177 2) , , , (48 c177 2) .9.1.3.2 ( 9.1.3.1) , - 9.1.2.3 , , , (24 c177 2) (48 c177 2) .9.1.4 (. , A.1) 9.1.3.1 9.1.3.2, (24 c177 2) (48 c177 2) , .9.2 (. , A.2)9.2.1 , , - , , . , , , , ( ).4 3187820129.2.2 9.2.2.1 . / , , - (, ). , - ISO 7218.9.2.2.2 5