EN ISO 16649-3-2015 en Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3 Detection and most probable n.pdf
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1、BSI Standards PublicationBS EN ISO 16649-3:2015Microbiology of the foodchain Horizontal methodfor the enumeration ofbeta-glucuronidase-positiveEscherichia coliPart 3: Detection and most probablenumber technique using 5-bromo-4-chloro-3-indolyl-D-glucuronideBS EN ISO 16649-3:2015 BRITISH STANDARDNati
2、onal forewordThis British Standard is the UK implementation of EN ISO 16649-3:2015. It supersedes DD ISO/TS 16649-3:2005 which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obt
3、ained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2015.Published by BSI Standards Limited 2015ISBN 978 0 580 79725 5 ICS 07.100.30 Compliance
4、with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2015.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dEUROPEAN STANDARD NORME EUROPENNE EUR
5、OPISCHE NORM EN ISO 16649-3 May 2015 ICS 07.100.30 English Version Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-D-glucuronide (ISO 16649-3
6、:2015) Microbiologie de la chane alimentaire - Mthode horizontale pour le dnombrement des Escherichia coli bta-glucuronidase positive - Partie 3: Recherche et technique du nombre le plus probable utilisant le bromo-5-chloro-4-indolyl-3 -D-glucuronate (ISO 16649-3:2015) Mikrobiologie der Lebensmittel
7、kette - Horizontales Verfahren zur Zhlung von -Glucuronidase-positiven Escherichia coli - Teil 3: Nachweis und Bestimmung der wahrscheinlichsten Keimzahl unter Verwendung von 5-Brom-4-Chlor-3-Indol-D-Glucuronid (ISO 16649-3:2015)This European Standard was approved by CEN on 16 April 2015. CEN member
8、s are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application
9、 to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Managemen
10、t Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Li
11、thuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17,
12、 B-1000 Brussels 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16649-3:2015 EBS EN ISO 16649-3:2015EN ISO 16649-3:2015 (E) 3 Foreword This document (EN ISO 16649-3:2015) has been prepared by Technical Committee ISO/TC 34
13、 “Foodproducts” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” thesecretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identicaltext or by endorsement, at the latest by Nov
14、ember 2015, and conflicting national standards shall be withdrawnat the latest by November 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patentrights. CEN and/or CENELEC shall not be held responsible for identifying any or all such paten
15、t rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, CzechRepublic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, Fra
16、nce, Germany, Greece,Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 16649-3:2015 has been approved by CEN as EN IS
17、O 16649-3:2015 without any modification. BS EN ISO 16649-3:2015ISO 16649-3:2015(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 24 Principle 24.1 Detection method . 24.2 Enumeration method 34.2.1 Inoculation of three or five tubes of double strength liquid sel
18、ective enrichment medium 5.3.1.1 a) with an equal volume of the test sample if the initial product is liquid, or with an equal volume of the initial suspension in the case of other products. 35 Dilution fluids and culture media . 35.1 General . 35.2 Dilution fluids 35.3 Culture media . 35.3.1 Minera
19、ls modified glutamate medium (selective enrichment medium) . 45.3.2 Tryptone bile glucoronide agar (second selective enrichment medium) . 55.3.3 Performance testing for the quality assurance of the culture media . 56 Apparatus and glassware 67 Sampling 68 Preparation of test sample . 79 Procedure. 7
20、9.1 Detection method . 79.1.1 Test portion, initial suspension, and dilutions . 79.1.2 Incubation of selective enrichment medium . 79.1.3 Subculturing 79.1.4 Secondary incubation 79.1.5 Examination of the plates . 79.1.6 Interpretation . 79.2 Enumeration method 79.2.1 Test portion, initial suspensio
21、n, and dilutions . 79.2.2 Inoculation of the selective enrichment medium 89.2.3 Incubation 89.2.4 Subculturing 89.2.5 Second incubation 89.2.6 Examination of the plates . 89.2.7 Interpretation . 810 Expression of results 910.1 Detection method . 910.2 Enumeration method 911 Precision . 912 Test repo
22、rt . 9Bibliography .10 ISO 2015 All rights reserved iiiContents PageBS EN ISO 16649-3:2015ISO 16649-3:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is n
23、ormally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part i
24、n the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular
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