EN ISO 12966-1-2014 en Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1 Guidelines on modern gas chromatography of fatty acid methyl est.pdf

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1、BSI Standards PublicationBS EN ISO 12966-1:2014Animal and vegetable fats andoils Gas chromatography offatty acid methyl estersPart 1: Guidelines on modern gaschromatography of fatty acid methyl estersBS EN ISO 12966-1:2014Incorporating corrigendum May 2015BS EN ISO 12966-1:2014 BRITISH STANDARDNatio

2、nal forewordThis British Standard is the UK implementation of EN ISO 12966-1:2014, incorporating corrigendum May 2015. It is identical to ISO 12966-1:2014. Together with BS EN ISO 12966-4, it supersedes BS EN ISO 5508:1995 and BS EN ISO 15304:2002 which will be withdrawn upon the publication of BS E

3、N ISO 12966-4.The UK participation in its preparation was entrusted to Technical Committee AW/307, Oilseeds, animal and vegetable fats and oils and their by-products.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to

4、 include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2015. Published by BSI Standards Limited 2015ISBN 978 0 580 90475 2ICS 67.200.10Compliance with a British Standard cannot confer immunity from legal obligations.T

5、his British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 January 2015.Amendments/corrigenda issued since publicationDate Text affected30 June 2015 Implementation of CEN corrigendum May 2015: Supersession information corrected in CEN title page and f

6、orewordEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 12966-1 December 2014 ICS 67.200.10 Supersedes EN ISO 5508:1995English Version Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl es

7、ters (ISO 12966-1:2014)Corps gras dorigines animale et vgtale - Chromatographie en phase gazeuse des esters mthyliques dacides gras - Partie 1: Lignes directrices relatives la chromatographie en phase gazeuse moderne des esters mthyliques dacides gras (ISO 12966-1:2014) Tierische und pflanzliche Fet

8、te und le - Gaschromatographie von Fettsuremethylestern - Teil 1: Leitfaden fr die moderne Gaschromatographie von Fettsuremethylestern (ISO 12966-1:2014) This European Standard was approved by CEN on 20 October 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which sti

9、pulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European

10、 Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are th

11、e national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romani

12、a, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by a

13、ny means reserved worldwide for CEN national Members. Ref. No. EN ISO 12966-1:2014 ESupersedes EN ISO 5508:1995 and EN ISO 15304:2002BS EN ISO 12966-1:2014EN ISO 12966-1:2014 (E) 3 Foreword This document (EN ISO 12966-1:2014) has been prepared by Technical Committee ISO/TC 34 “Food products“ in coll

14、aboration Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical t

15、ext or by endorsement, at the latest by June 2015, and conflicting national standards shall be withdrawn at the latest by June 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible

16、for identifying any or all such patent rights. This document supersedes EN ISO 5508:1995. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Cze

17、ch Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdo

18、m. Endorsement notice The text of ISO 12966-1:2014 has been approved by CEN as EN ISO 12966-1:2014 without any modification. BS EN ISO 12966-1:2014EN ISO 12966-1:2014 (E) 3 Foreword This document (EN ISO 12966-1:2014) has been prepared by Technical Committee ISO/TC 34 “Food products“ in collaboratio

19、n Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or b

20、y endorsement, at the latest by June 2015, and conflicting national standards shall be withdrawn at the latest by June 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for iden

21、tifying any or all such patent rights. This document supersedes EN ISO 5508:1995. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Repub

22、lic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endor

23、sement notice The text of ISO 12966-1:2014 has been approved by CEN as EN ISO 12966-1:2014 without any modification. BS EN ISO 12966-1:2014EN ISO 12966-1:2014 (E) 3 Foreword This document (EN ISO 12966-1:2014) has been prepared by Technical Committee ISO/TC 34 “Food products“ in collaboration Techni

24、cal Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endors

25、ement, at the latest by June 2015, and conflicting national standards shall be withdrawn at the latest by June 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying

26、any or all such patent rights. This document supersedes EN ISO 5508:1995. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Den

27、mark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement n

28、otice The text of ISO 12966-1:2014 has been approved by CEN as EN ISO 12966-1:2014 without any modification. This document supersedes EN ISO 5508:1995 and EN ISO 15304:2002.BS EN ISO 12966-1:2014ISO 12966-1:2014(E) ISO 2014 All rights reserved iiiContents PageForeword ivIntroduction v1 Scope . 12 No

29、rmative references 13 Principle 14 Preparation of FAME . 25 Columns . 36 GLC of FAMEs . 47 Evaluation of the chromatograms 47.1 Peak area and area per cent . 47.2 Evaluation by means of an internal standard or correction factors . 48 Test report . 5Bibliography 6BS EN ISO 12966-1:2014ISO 12966-1:201

30、4(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which

31、a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matt

32、ers of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This doc

33、ument was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all s

34、uch patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and do

35、es not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary inform

36、ationThe committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils.This first edition of ISO 12966-1, together with ISO 12966-4, cancels and replaces ISO 5508:1990 and ISO 15304:2002 which have been technically revised.ISO 12966 consi

37、sts of the following parts, under the general title Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters: Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters Part 2: Preparation of methyl esters of fatty acids Part 3: Preparation of methyl esters

38、using trimethylsulfonium hydroxide (TMSH) Part 4: Capillary gas chromatographic methodiv ISO 2014 All rights reservedBS EN ISO 12966-1:2014ISO 12966-1:2014(E)IntroductionThis part of ISO 12966 is one of a suite of four International Standards for the preparation and determination of fatty acid methy

39、l esters by gas chromatography in animal and vegetable fats and oils. ISO 12966 (all parts) is applicable to crude, refined, partially hydrogenated or fully hydrogenated fats, oils and fatty acids derived from animal and vegetable sources.ISO 12966 (all parts) is not suitable for the analysis of dai

40、ry, ruminant fats and oils (including milk and milk products or fat coming from milk and milk products), or products supplemented with conjugated linoleic acid (CLA). Furthermore it is not intended to be applied to polymerized and oxidized fats and oils.This part of ISO 12966 is a guideline to the m

41、odern gas chromatography of fatty acid methyl esters, while ISO 12966-2 and ISO 12966-3 cover the preparation of fatty acid methyl esters by different methods. In ISO 12966-4, the conditions for the analysis of fatty acid methyl esters by capillary gas chromatography are given.This suite of Internat

42、ional Standards replaces the following International Standards: ISO 5508:1990 is replaced by ISO 12966-1 and ISO 12966-4 ISO 15304:2002 is replaced by ISO 12966-1 and ISO 12966-4 ISO 5509:2000 is replaced by ISO 12966-2 and ISO 12966-3 ISO 2014 All rights reserved vBS EN ISO 12966-1:2014BS EN ISO 12

43、966-1:2014Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters1 ScopeThis part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, free and bound, in animal an

44、d vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEs).The qualitative and quantitative determination of the composition of fatty acids by gas liquid chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of fats and

45、 oils, or fatty foodstuffs after the extraction of the oil from the matrix. The bound fatty acids of the triacylglycerols (TAGs) and, depending on the esterification method, the free fatty acids (FFA) and other lipids, are converted to fatty acid methyl esters (FAMEs), which are determined by capill

46、ary gas chromatography. Depending on the number of different fatty acids (theoretically more than 50 different fatty acids can be present) capillary columns with a length of 10 m to 100 m are used for a separation.The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and

47、monoacylglycerols, to fatty acid esters, free fatty acids, soaps and other fatty compounds. With this suite of standards, FAMEs from C4 to C26 can be determined, including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated

48、fatty acid methyl esters.For the determination of short chain fatty acids, isopropyl and butyl esters are often used so as to avoid interferences with the solvent peak and in order to reduce differences in detector responses.2 Normative referencesThe following documents, in whole or in part, are nor

49、matively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 12966-2, Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 2: Preparation of methyl esters of fatty acidsISO 12966-3, Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 3: Preparation of methyl esters using trimethylsulfoni