DIN EN ISO 16265-2012 Water quality - Determination of the methylene blue active substances (MBAS) index - Method using continuous flow analysis (CFA) (ISO 16265 2009) German versi.pdf

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1、May 2012 Translation by DIN-Sprachendienst.English price group 11No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13

2、.060.50!$|04“1891317www.din.deDDIN EN ISO 16265Water quality Determination of the methylene blue active substances (MBAS) index Method using continuous flow analysis (CFA) (ISO 16265:2009)English translation of DIN EN ISO 16265:2012-05Wasserbeschaffenheit Bestimmung des Indexes von methylenblauaktiv

3、en Substanzen (MBAS) Verfahren mittels kontinuierlicher Durchflussanalyse (CFA) (ISO 16265:2009)Englische bersetzung von DIN EN ISO 16265:2012-05Qualit de leau Mesurage de lindice des substances actives au bleu de mthylne (SABM) Mthode par analyse en flux continu (CFA) (ISO 16265:2009)Traduction ang

4、laise de DIN EN ISO 16265:2012-05www.beuth.deDocument comprises 19 pagesIn case of doubt, the German-language original shall be considered authoritative.04.12 DIN EN ISO 16265:2012-05 2 A comma is used as the decimal marker. National foreword This standard has been prepared by Technical Committee IS

5、O/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-03-01-05 AK Fliean

6、alysenverfahren of NA 119-01-03 AA Wasseruntersuchung. This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung Summarische Wirkungs- und Stoffkenngren (Gruppe H) (German standard methods for the examination of water, waste water and sludge Parame

7、ters characterizing effects and substances (group H) and describes method H 58. Designation of the method: Determination of the methylene blue active substances (MBAS) index Method using continuous flow analysis (CFA) (H 58): Method DIN EN ISO 16265 H 58 This standard has been published to implement

8、 the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy1). The DIN Standards corresponding to the International Standards referred to in this document are as

9、 follows: ISO 648 DIN EN ISO 648 ISO 1042 DIN EN ISO 1042 ISO 3696 DIN ISO 3696 ISO 5725-2 DIN ISO 5725-2 ISO 8466-1 DIN 38402-51 ISO 8466-2 DIN ISO 8466-2 1)Registered in the DITR database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 10772 Berlin. DIN EN ISO 16265:2012-05 3 Expert assi

10、stance and specialized laboratories will be required to perform the analysis described in this standard. Existing safety regulations are to be taken into account. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what extent additional con

11、ditions will have to be specified. German standard methods for the examination of water, waste water and sludge Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods included in the loose-leaf publication en

12、titled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published jointly by Wiley-VCH Verlag and Beuth Verlag GmbH. All standard methods relevant to the Abwasserverordnung (Waste Water Regulation) (AbwV2) included in the new Regulation on Section 57, par

13、agraph 1, number 1, of the Gesetz zur Ordnung des Wasserhaushaltes (German Water Management Act2) concerning Anforderungen an das Einleiten von Abwasser in Gewsser together with the Abwasserverordnung and the Gesetz zur Ordnung des Wasserhaushalts have been published by Beuth Verlag GmbH as a loose-

14、leaf collection Analysenverfahren in der Abwasserverordnung Rechtsvorschriften und Normen (Supplements). Standards or draft standards bearing the group title “German standard methods for the examination of water, waste water and sludge” are classified under the following categories (main titles): Ge

15、neral information (group A) (DIN 38402) Sensory analysis (group B) (DIN 38403) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 38406) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters

16、 characterizing effectsand substances (group H) (DIN 38409) Biological-ecological methods of analysis (group M) (DIN 38410) Microbiological methods (group K) (DIN 38411) Test methods using water organisms (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group

17、 S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) In addition to the methods described in the DIN 38402 to DIN 38415 series of standards, there are a number of European and International Standards available as DIN EN, DIN EN ISO and DIN ISO Standards, which also form part of the c

18、ollection of German standard methods. Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenausschuss Wasserwesen, telephone +49 30 2601-2448, or from Beuth Verlag GmbH, Burggrafenstr. 6, 10787 Berlin. 2)Registered in the DIT

19、R database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 10772 Berlin. DIN EN ISO 16265:2012-05 4 National Annex NA (informative) Bibliography DIN 38402-51, German standard methods for the examination of water, waste water and sludge General information (group A) Calibration of analytica

20、l methods, evaluation of analytical results and linear calibration functions used to determine the performance characteristics of analytical methods (A 51) DIN EN ISO 648, Laboratory glassware Single-volume pipettes DIN EN ISO 1042, Laboratory glassware One-mark volumetric flasks DIN ISO 3696, Water

21、 for analytical laboratory use Specification and test methods DIN ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method DIN ISO 8466-2, Water quality Calibratio

22、n and evaluation of analytical methods and estimation of performance characteristics Part 2: Calibration strategy for non-linear second-order calibration functions EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 16265 February 2012 ICS 13.060.50 English Version Water quality - Determination

23、 of the methylene blue active substances (MBAS) index - Method using continuous flow analysis (CFA) (ISO 16265:2009) Qualit de leau - Mesurage de lindice des substances actives au bleu de mthylne (SABM) - Mthode par analyse en flux continu (CFA) (ISO 16265:2009) Wasserbeschaffenheit - Bestimmung des

24、 Indexes von methylenblauaktiven Substanzen (MBAS) - Verfahren mittels kontinuierlicher Durchflussanalyse (CFA) (ISO 16265:2009) This European Standard was approved by CEN on 15 January 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for

25、 giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three

26、 official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodi

27、es of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and Un

28、ited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16265:

29、2012: EContents Page Foreword. 3 Introduction 4 1 Scope . 5 2 Normative references . 5 3 Interferences . 5 4 Principle. 6 5 Reagents 6 6 Apparatus 8 7 Sampling and sample pretreatment 9 8 Procedure 9 9 Calculation of the results. 11 10 Expression of results . 12 11 Precision 12 12 Test report . 12 A

30、nnex A (informative) Example of a flow diagram for the determination of MBAS index by continuous flow analysis (CFA) 13 Annex B (informative) Precision data. 14 Bibliography . 15 EN ISO 16265:2012 (E) DIN EN ISO 16265:2012-05 2Foreword The text of ISO 16265:2009 has been prepared by Technical Commit

31、tee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 16265:2012 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard,

32、 either by publication of an identical text or by endorsement, at the latest by August 2012, and conflicting national standards shall be withdrawn at the latest by August 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN a

33、nd/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Cz

34、ech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO

35、16265:2009 has been approved by CEN as a EN ISO 16265:2012 without any modification. EN ISO 16265:2012 (E) DIN EN ISO 16265:2012-05 3 Introduction Methods using flow analysis automate wet chemical procedures and are particularly suitable for the processing of many analytes in water in large numbers

36、of samples at a high analysis frequency (up to 100 samples per hour). A differentiation is made between flow injection analysis (FIA)1, 2and continuous flow analysis (CFA)3. Both methods share the feature of an automatic dosage of the sample into a flow system (manifold) where the analytes in the sa

37、mple react with the reagent solutions on their way through the manifold. The sample preparation may be integrated in the manifold. The reaction product is measured in a flow detector (e.g. a photometer). The detector produces a signal from which the concentration of the parameter is calculated. The

38、MBAS (methylene blue active substances) index is an analytical convention (a method-defined parameter) used for water quality control purposes. It measures surfactants and other substances that react with methylene blue under specified conditions. The user should be aware that particular problems co

39、uld require the specification of additional conditions. EN ISO 16265:2012 (E) DIN EN ISO 16265:2012-05 4WARNING Persons using this International Standard should be familiar with normal laboratory practice. This standard does not purport to address all of the safety problems, if any, associated with

40、its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted in accordance with this International Standard be carried out by suitably tra

41、ined staff. Trichloromethane and methanol waste solutions should be disposed of properly. 1 Scope This International Standard specifies a procedure for the determination of the methylene blue active substances (MBAS) index, in the ranges 0,05 mg/l to 0,5 mg/l and 0,5 mg/l to 5,0 mg/l, in various wat

42、er samples (e.g. ground water, drinking water, surface water, waste water and leachates). Anionic surfactants are the most important substances showing methylene blue activity. This method is therefore useful for estimating the anionic surfactant content including anionic surfactants with carboxylat

43、e groups (e.g. soaps) of water. Other types of substance may also show methylene blue activity and contribute to the result. On a case-by-case basis, the range of the analysis may be changed and the method used for other concentration ranges provided they cover exactly one decade of concentration un

44、its. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the reference document (including any amendments) applies. ISO 648, Laboratory gl

45、assware Single-volume pipettes ISO 1042, Laboratory glassware One-mark volumetric flasks ISO 3696, Water for analytical laboratory use Specification and test methods 3 Interferences The following substances can interfere with the analysis: Cationic compounds able to form strong ion-pairs with the ac

46、tive substances in methylene blue. Humic acids in concentrations 20 mg/l. Chemicals with a high surface activity (e.g. non methylene blue active surfactants in concentrations 50 mg/l). EN ISO 16265:2012 (E) DIN EN ISO 16265:2012-05 5 Chemicals with a strong reductive potential for the oxidation of m

47、ethylene blue (e.g. S2, 23SO, 223SO ,OCl). These chemicals shall be removed (e.g. with H2O2) prior to analysis. High concentrations of inorganic anions (e.g. nitrate, chloride, bromide) may cause positive bias. Chemicals which react with any of the reagents used to form a coloured compound soluble i

48、n trichloromethane (CHCl3) (excluded are methylene blue active substances). Filtration of the sample before analysis is advisable for samples containing particles larger than 100 m in size. Otherwise the particles in the sample may clog the transport tubes. If filtration is necessary, significant losses of anionic surf