DIN EN ISO 14501-2008 Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromato.pdf
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1、January 2008DEUTSCHE NORM English price group 10No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 67.100.10!$K_M“1406042
2、www.din.deDDIN EN ISO 14501Milk and milk powder Determination of aflatoxin M1 content Clean-up by immunoaffinity chromatography and determination byhigh-performance liquid chromatography (ISO 14501:2007)English version of DIN EN ISO 14501:2008-01Milch und Milchpulver Bestimmung des Gehalts an Aflato
3、xin M1 Reinigung durch Immunaffinitts-Chromatographie und Bestimmung mitHochleistungs-Flssigchromatographie (ISO 14501:2007)Englische Fassung DIN EN ISO 14501:2008-01SupersedesDIN EN ISO 14501:1999-01www.beuth.deDocument comprises 14 pagesDIN EN ISO 14501:2008-01 2 National foreword This standard fa
4、lls in the domain of Technical Committee CEN/TC 302 “Milk and milk products Methods of sampling and analysis” (Secretariat: NEN, the Netherlands). It is based on International Standard ISO 22160:2007 which was prepared by Technical Committee ISO/TC 34 “Food products” (Secretariat: AFNOR, France), Su
5、bcommittee SC 5 “Milk and milk products” (Secretariat: NEN, the Netherlands). Based on the results of parallel voting, ISO 14501:2007 has been adopted as European Standard. The responsible German body involved in its preparation was the Normenausschuss Lebensmittel und land-wirtschaftliche Produkte
6、(Food and Agricultural Products Standards Committee), Technical Committee Milch und Milchprodukte Probenahme- und Untersuchungsverfahren. The DIN Standards corresponding to the International Standard referred to in the bibliography of the EN are as follows: ISO 707 DIN EN ISO 707 ISO 5725-1 DIN ISO
7、5725-1 ISO 5725-2 DIN ISO 5725-2 Amendments This standard differs from DIN EN ISO 14501:1999-01 as follows: a) The test is now carried out using acetonitrile instead of chloroform. b) The results of the interlaboratory trial have been revised (see Annex A). c) The standard has been editorially revis
8、ed to take account of the new style rules for standards. Previous editions DIN EN ISO 14501: 1999-01 National Annex NA (informative) Bibliography DIN EN ISO 707, Milk and milk products Guidance on sampling DIN ISO 5725-1, Accuracy (trueness and precision) of measurement methods and results Part 1: G
9、eneral principles and definitions DIN ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 14501 Octob
10、er 2007 ICS 67.100.10 Supersedes EN ISO 14501:1998English Version Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography (ISO 14501:2007) Lait et lait en poudre - Dtermination de la teneur e
11、n aflatoxine M1 - Purification par chromatographie dimmunoaffinit et dtermination par chromatographie en phase liquide haute performance (ISO 14501:2007) Milch und Milchpulver - Bestimmung des Gehalts an Aflatoxin M1- Reiningung durch Immunaffinitts-Chromatographie und Bestimmung mit Hochleistungs-F
12、lssigchromatographie (ISO 14501:2007) This European Standard was approved by CEN on 13 October 2007. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up
13、-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation u
14、nder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece
15、, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management
16、 Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 14501:2007: EForeword This document (EN ISO 14501:2007) has been prepared by Technical Committee ISO/TC 34 “Food products” in col
17、laboration with Technical Committee CEN/TC 302 “Milk and milk products Methods of sampling and analysis” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by Ap
18、ril 2008, and conflicting national standards shall be withdrawn at the latest by April 2008. This document supersedes EN ISO 14501:1998. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: A
19、ustria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endors
20、ement notice The text of ISO 14501:2007 has been approved by CEN as a EN ISO 14501:2007 without any modification. 2 EN ISO 14501:2007 (E) DIN EN ISO 14501:2008-01 1 Scope This International Standard specifies a method for the determination of aflatoxin M1content in milk and milk powder. The limit of
21、 detection is 0,08 g/kg for whole milk powder, i.e. 0,008 g/l for reconstituted liquid milk. The method is also applicable to low fat milk, skimmed milk, low fat milk powder, and skimmed milk powder. CAUTION 1 The method described in this protocol requires the use of solutions of aflatoxin M1. Aflat
22、oxins are carcinogenic to humans. Attention is drawn to the statement made by the International Agency for Research on Cancer 4, 5. 2 Protect the laboratory in which the analyses are performed adequately from daylight and keep aflatoxin standard solutions protected from light, e.g. by using aluminiu
23、m foil. 3 The use of non-acid-washed glassware (e.g. tubes, vials, flasks, beakers, syringes) for aqueous aflatoxin solutions may cause loss of aflatoxin. Moreover, brand new laboratory glassware, before coming into contact with aqueous solutions of aflatoxin, should be soaked in dilute acid (e.g. s
24、ulfuric acid, 2 mol/l) for several hours, then rinsed well with distilled water to remove all traces of acid (check to ensure pH is in the range 6 to 8). 4 Use decontamination procedures for laboratory wastes such as solid compounds, solutions in organic solvents, aqueous solutions and spills, and f
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