DIN EN ISO 11269-2-2013 Soil quality - Determination of the effects of pollutants on soil flora - Part 2 Effects of contaminated soil on the emergence and early growth of higher pl.pdf
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1、May 2013Translation by DIN-Sprachendienst.English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.
2、080.30!%$/“2011259www.din.deDDIN EN ISO 11269-2Soil quality Determination of the effects of pollutants on soil flora Part 2: Effects of contaminated soil on the emergence and early growthof higher plants (ISO 11269-2:2012);English version EN ISO 11269-2:2013,English translation of DIN EN ISO 11269-2
3、:2013-05Bodenbeschaffenheit Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora Teil 2: Wirkung von verunreinigten Bden auf Saatauflauf und frhes Wachstum hhererPflanzen (ISO 11269-2:2012);Englische Fassung EN ISO 11269-2:2013,Englische bersetzung von DIN EN ISO 11269-2:2013-05Qualit du sol
4、 Dtermination des effets des polluants sur la flore du sol Partie 2: Effets des sols contamins sur lmergence et la croissance des vgtauxsuprieurs (ISO 11269-2:2012);Version anglaise EN ISO 11269-2:2013,Traduction anglaise de DIN EN ISO 11269-2:2013-05SupersedesDIN ISO 11269-2:2006-09www.beuth.deIn c
5、ase of doubt, the German-language original shall be considered authoritative.Document comprises 27 pages04.13 DIN EN ISO 11269-2:2013-05 2 A comma is used as the decimal marker. National foreword The text of ISO 11269-2:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” and has
6、been taken over as EN ISO 11269-2:2013 by Technical Committee CEN/TC 345 “Characterization of soils” (Secretariat: NEN, Netherlands). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-02-04 UA
7、 Biologische Verfahren of NA 119-01-02 AA Abfall- und Bodenuntersuchungen. ISO 11269 consists of the following parts, under the general title Soil quality Determination of the effects of pollutants on soil flora: Part 1: Method for the measurement of inhibition of root growth Part 2: Effects of cont
8、aminated soil on the emergence and early growth of higher plants Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 10381-6 DIN
9、 ISO 10381-6 ISO 10390 DIN ISO 10390 ISO 10694 DIN ISO 10694 ISO 11260 DIN EN ISO 11260 ISO 11268-1 DIN ISO 11268-1 ISO 11268-2 DIN ISO 11268-2 ISO 11274 DIN ISO 11274 ISO 11277 DIN ISO 11277 ISO 11465 DIN ISO 11465 ISO 22030 DIN EN ISO 22030 Amendments This standard differs from DIN ISO 11269-2:200
10、6-09 as follows: a) ISO 112692 has been taken over as European Standard EN ISO 11269-2; b) the method is now applicable to soils and contaminated soils; c) Annex A “Additional recommended plant species based on test results gained by applying Environment Canada Test Method: EPS 1/RM/45” (Biological
11、test method: Test for measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in Soil) has been added; d) Annex B “Phytotoxic values for reference compounds: sodium trichloro-acetate and boric acid” has been supplemented; e) Annex D “Recommendations for nutrient supply of soils”
12、 has been added. Previous editions DIN ISO 11269-2: 1997-10, 2006-09 DIN EN ISO 11269-2:2013-05 3 National Annex NA (informative) Bibliography DIN ISO 11260, Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution DIN EN ISO 22030, So
13、il quality Biological methods Chronic toxicity in higher plants DIN ISO 10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory DIN ISO 10390, Soi
14、l quality Determination of pH DIN ISO 10694, Soil quality Determination of organic and total carbon after dry combustion (elementary analysis) DIN ISO 11268-1, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 1: Determination of acute toxicity using artificial soil substrate DI
15、N ISO 11268-2, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 2: Determination of effects on reproduction DIN ISO 11274, Soil quality Determination of the water retention characteristics Laboratory methods DIN ISO 11277, Soil quality Determination of particle size distributio
16、n in mineral soil material Method by sieving and sedimentation DIN ISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravimetric method DIN EN ISO 11269-2:2013-05 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11269-2 F
17、ebruary 2013 ICS 13.080.30 English Version Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants (ISO 11269-2:2012) Qualit du sol - Dtermination des effets des polluants sur la flore du sol -
18、Partie 2: Effets des sols contamins sur lmergence et la croissance des vgtaux suprieurs Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora - Teil 2: Wirkung von verunreinigten Bden auf Saatauflauf und frhes Wachstum hherer Pflanzen (ISO 11269-2:2012) This European Sta
19、ndard was approved by CEN on 5 February 2013. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concernin
20、g such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into it
21、s own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germa
22、ny, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR
23、NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11269-2:2013: E(ISO 11269-2:2012) Contents Page Foreword . 3 Introduction . 4 1 Scope . 5 2 Normative references.
24、 5 3 Terms and definitions 5 4 Units. 7 5 Principle 7 6 Test plants 7 7 Materials 8 7.1 Test vessels 8 7.2 Soil . 8 8 Equipment . 10 9 Reference substance . 10 10 Procedure 10 10.1 Experimental design 10 10.2 Preparation of the pots 11 10.3 Preparation of the seeds . 11 10.4 Growth conditions 12 10.
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